We narrowed to 26,677 results for: Cat
-
Plasmid#135401PurposeExpresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activityDepositorInsertDNAse II alpha (DNASE2 Human)
UseLentiviralTagsT2A, mCherry, and sfGFPExpressionMammalianMutationSingle-nucleotide polymorphism C432TPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS
Plasmid#175006PurposeAkt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.DepositorInsertmCherry-Akt-STOPS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTag-BFP-C-h-Rab7L1-c-Myc
Plasmid#79803PurposeExpress TagBFP labeled human Rab7L1.DepositorInsertRab7L1 (RAB29 Human)
UseTagsTagBFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
SpRY Cas9
Plasmid#188491PurposeExpresses FLAG tagged SpRY Cas9DepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationA61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…PromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-ccdB
Plasmid#166145PurposeGateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.DepositorTypeEmpty backboneUseTagsHis10ExpressionBacterialMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTag-RFP-C-h-Rab7L1-c-Myc
Plasmid#79804PurposeExpress TagRFP labeled human Rab7L1.DepositorInsertRab7L1 (RAB29 Human)
UseTagsTagRFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV2-hSyn-G-Flamp1
Plasmid#188569Purposegreen biosensor for cAMP in neuronsDepositorInsertG-Flamp1
UseAAVTagsHis tagExpressionMutationPromoterhSynAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
retro-gRNA-eGFP
Plasmid#116926PurposeRetrovirus expressing eGFP with BBSI cloning sites for sgRNADepositorInserteGFP
UseRetroviralTagsExpressionMutationPromoterEF1aAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
XRE-pNL1.3[secNLuc]
Plasmid#182295PurposeSecreted Luciferase reporter for AhR activityDepositorInsertXenobiotic Response Element (CYP1A1 Human)
UseTagssecNlucExpressionMammalianMutationPromoterXREAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-acceptor-2xBbsI
Plasmid#205633PurposeCloning and U6-drive expression of ISDra2 TnpB reRNAsDepositorInsertISDra2-TnpB reRNA with placeholder for guide cloning with BbsI
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES
Plasmid#200402PurposeEntry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detectionDepositorInsertClosed-loop DART VADAR sensor entry cassette
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4 GlyR IRES EGFP
Plasmid#119739PurposeChemogenetic inhibitor expression plasmidDepositorInsertPSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
UseTagsExpressionMammalianMutationL131G, Q139L, Y217FPromoterCAGAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4 5HT3 LC IRES GFP
Plasmid#123066PurposeChemogenetic activator expression plasmidDepositorInsertPSAM4 5HT3 LC IRES GFP (Htr3a Mouse)
UseTagsExpressionMammalianMutationL131G Q139L Y217FPromoterCAGAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Beta1-GFP in pHcgreen
Plasmid#69804PurposeExpresses Beta1-GFP integrin for normal cell transfectionDepositorInsertBeta1A integrin (ITGB1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_ORI_empty
Plasmid#99297PurposeLuciferase validation vector; empty vector without insert to determine baselineDepositorTypeEmpty backboneUseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pT077
Plasmid#137879PurposeDonor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFPDepositorInsertTRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag hHMGB1
Plasmid#31609DepositorInsertHMGB1 (HMGB1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
MuHKII-pGFPN3
Plasmid#21922DepositorInsertMutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)
UseTagsGFPExpressionMammalianMutationThis is the full length human HKII cDNA sequence …PromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
TrHKII-pGFPN3
Plasmid#21921DepositorInsertTruncated human HKII cDNA (lacking the sequence encoded by exon 1, which is the mitochondrial binding domain) in pGFP-N3 plasmid (HK2 Human)
UseTagsGFPExpressionMammalianMutationThis is the truncated human HKII cDNA sequence (l…PromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSPneogRNA241510+MT
Plasmid#84292PurposeExpress LdPBK_241510.1 and LdMT targeting gRNAs simutaneouslyDepositorInsertLdBPK_241510.1 targeting gRNA and LdMT targeting gRNA
UseCRISPR; Leishmania donovaniTagsExpressionMutationPromoterL. donovani ribosome RNA promoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1
Plasmid#233078PurposeExpression vector for sgRNA-S1b and eCas12f1DepositorInsertU6-sgRNA-S1b (BsaI)-CBh-bpNLS-eCas12f1-2xFLAG-P2A-EGFP
UseCRISPRTags2xFLAGExpressionMammalianMutationPromoterhU6, CBhAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc wt MBS1-4
Plasmid#16564DepositorInsertc-MYC binding sites (MYC Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET21 gamma-tubulin
Plasmid#101825Purposeproduction of gamma-tubulin in bacteriaDepositorInsertgamma-tubulin 1 (TUBG1 Human)
UseTagsHisExpressionBacterialMutationWhole proteinPromoterT7Available sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcw107-V5
Plasmid#64649Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorInsertn/a
UseLentiviralTagsV5ExpressionMutationPromoterPGKAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUCas9Cherry
Plasmid#70182PurposeExpresses Cas9 with fluorescent Cherry reporterDepositorInserthumanised S.pyogenes cas9
UseCRISPR and LentiviralTags3xFLAGExpressionMammalianMutationPromoterhUbCAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
p300 KAT LIC 1M
Plasmid#233587PurposeBacterial expression of p300 KAT enzymeDepositorInsertp300 KAT (EP300 Human)
UseTags6xHis MBPExpressionBacterialMutationPromoterT7Available sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
sUPRa
Plasmid#223242PurposesUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levelsDepositorInsertsUPRa
UseAAVTagsExpressionMutationPromoterCustomAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMJ922
Plasmid#78312PurposeExpression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coliDepositorInsertSpCas9
UseTagsHA-2xNLS-EGFP-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMax_mOrange_G299C
Plasmid#177829PurposeSite-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).DepositorInsertmOrange_G299C
UseTagsExpressionMammalianMutationG299CPromoterCMVAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-E2-25K
Plasmid#63572PurposeExpression of human Ubiquitin conjugating enzyme E2-25KDepositorInsertUBE2-25K (UBA7 Human)
UseTagsGSTExpressionBacterialMutationIsoform 1 (200 resiudes)PromotertacAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT076
Plasmid#137880PurposeDonor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFPDepositorInsertTRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDL025_HA-rb_IgG_HC_15F11
Plasmid#193622PurposeHA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.DepositorInsertAnti_HA_15F11 rabbit heavy chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDL026_HA-rb_IgG_LC_15F11
Plasmid#193623PurposeHA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.DepositorInsertAnti-HA_15F11 rabbit light chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLJM60 FLAG-RAB35 S22N
Plasmid#107106PurposeLentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.DepositorInsertRAB35 (RAB35 Human)
UseLentiviralTagsFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG FUCCI
Plasmid#136934Purposedonor plasmid for constitutive FUCCI expression in human cellsDepositorInsertClover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
UseSynthetic BiologyTagsClover and mKO2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WHaloCaMP1a-EGFP
Plasmid#205313PurposeExpression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoterDepositorInsertWHaloCaMP1a-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK-FLAG-UbxD8
Plasmid#53777Purposeexpress FLAG-tagged human UbxD8 geneDepositorInsertUbxD8 (FAF2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRelA*
Plasmid#175590PurposeTet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
UseTagsFused with a flexible GS linker to mVenusExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMuLE Lenti Dest eGFP
Plasmid#62175PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses EGFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
keratin 8 (krt8)-H2B-RFP
Plasmid#105955PurposetransgenesisDepositorInsertkrt8 (KRT8 Human)
UseTagsRFPExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDL023_Tub-rb_IgG_HC
Plasmid#193620Purposeα-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.DepositorInsertAnti-Tubulin scFv heavy variable + rabbit constant chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMuLE Lenti Dest iRFP
Plasmid#62176PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses iRFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDL024_Tub-rb_IgG_LC
Plasmid#193621Purposeα-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.DepositorInsertAnti-Tubulin scFv light variable + rabbit constant chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEasiLV-MCS-coICP0
Plasmid#235545PurposeTo express ICP0 (doxycycline inducible)DepositorInsertICP0
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i
Plasmid#233080PurposeExpression vector for sgRNA-S1b and eCas12f1iDepositorInsertU6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP
UseCRISPRTags2xFLAGExpressionMammalianMutationPromoterhU6, CBhAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a
Plasmid#233079PurposeExpression vector for sgRNA-S1b and eCas12f1aDepositorInsertU6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1a-2xFLAG-P2A-EGFP
UseCRISPRTags2xFLAGExpressionMammalianMutationPromoterhU6, CBhAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMCP-Luc (MCP-1 promoter in pGL3-basic)
Plasmid#40324DepositorInsertMCP-1 promoter (Ccl2 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationcontains MCP-1 promoter region through the nucleo…PromoterMCP-1Available sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
Lyn-FKBP-FKBP-CFP (LF2C)
Plasmid#20149DepositorInsertLyn-FKBP-FKBP-CFP (FKBP1A Human)
UseTagsExpressionMammalianMutationSee depositor comments below.PromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUltra-sY
Plasmid#82417PurposeExpresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codonsDepositorInsertMethanococcus jannaschii aaRS for sulfotyrosine
UseTagsExpressionBacterialMutationTyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…PromotertacIAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only