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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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8,789 results

Showing: 1241 - 1280 of 8789 results

  1. pSG5 Large T

    Plasmid
    #9053
    Depositor
    William Hahn
    Insert
    large T antigen (SV40gp6 simian virus)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 5, 2006
    Availability
    Academic Institutions and Nonprofits only
    9053-plasmid-map-sequence-id-244756

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  2. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available Since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  7. pFLAG-CMV-hErk1

    Plasmid
    #49328
    Purpose
    Expresses FLAG-tagged human Erk1
    Depositor
    Melanie Cobb
    Insert
    Erk1 (MAPK3 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    49328-plasmid-map-sequence-id-148204

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  18. pThermoCas9_ctrl

    Plasmid
    #100981
    Purpose
    Expresses ThermoCas9 and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available Since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100981-plasmid-map-sequence-id-196605

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  19. EF1a mCherry

    Plasmid
    #129431
    Purpose
    expression of mCherry from EF1a promoter
    Depositor
    Oskar Laur
    Insert
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129431-plasmid-map-sequence-id-256111

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  21. pET21b(+)-Is-PETase

    Plasmid
    #112202
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    Promoter
    N/A
    Available Since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112202-plasmid-map-sequence-id-334619

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  26. lenti MS2-P65-HSF1_Hygro

    Plasmid
    #61426
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Insert
    MS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1A
    Available Since
    Dec. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    61426-plasmid-map-sequence-id-140565

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  29. lenti-sgRNA neo

    Plasmid
    #104992
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104992-plasmid-map-sequence-id-213507

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  38. pCMV-T7-EGFP (BPK1098)

    Plasmid
    #133962
    Purpose
    CMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcription
    Depositor
    Benjamin Kleinstiver
    Insert
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available Since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    133962-plasmid-map-sequence-id-265771

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  40. PB-sgRNA(MS2)

    Plasmid
    #102560
    Purpose
    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102560-plasmid-map-sequence-id-198595

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Showing: 1241 - 1280 of 8789 results