We narrowed to 23,686 results for: Spr
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Plasmid#128160PurposePtet-controlled expression of S. pyogenes Cas9DepositorInsertSp Cas9
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJJB296
Plasmid#107691Purpose35SPromoter-LwaCas13a-HSPTerminatorDepositorInsertCas13a
UseTagsExpressionPlantMutationPromoter35SAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDonor_sU6
Plasmid#69351PurposesgRNA scaffold and synthetic U6 promoterDepositorInsertsU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRTagsExpressionMutationPromotersynthetic U6 promoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
KO plasmid
Plasmid#135970PurposeExpresses SpCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJJB308
Plasmid#107699PurposeAtU6 Promoter-LwaCas13a-gRNA ScaffoldDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-U6insert
Plasmid#104536PurposeA piggybac vector with a U6 promoter that allows cloning of guide RNAsDepositorTypeEmpty backboneUseCRISPR, Mouse Targeting, and Synthetic Biology ; …TagsExpressionMammalianMutationPromoterU6Available sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL3287
Plasmid#139750PurposeBase Cas12a CRISPRi plasmidDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
gRNA-eGFP-Reporter
Plasmid#60719PurposeExpresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.DepositorInsertgRNA (5'-AAAGGTCGAGAAACTGCAAA-3')
UseCRISPRTagsExpressionMammalianMutationPromoterU6 and mouse U6Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX458_RAD21_1
Plasmid#64057PurposeExpresses gRNA against human RAD21 along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX458_CREB1_1
Plasmid#64939PurposeExpresses gRNA against human CREB1 along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJA42
Plasmid#59930PurposegRNA for cleavage at rol-6(su1006) locus in C elegansDepositorInsertrol-6(su1006) gRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJF093
Plasmid#113323PurposeSp.pCas9-dCas9, pTet-MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRTagsExpressionBacterialMutationR93APromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFD7
Plasmid#73916PurposegRNA vector for AsCpf1DepositorInserttRNA:hAsCpf1-gRNA:tRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRE57-Ter
Plasmid#170635PurposeDNA donor for transpositionDepositorInsertMini-Tn6677, Ter cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4280
Plasmid#98699PurposeA Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 systemDepositorInsertCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4281
Plasmid#98700PurposeA Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX systemDepositorInsertCas9
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMVP-Cas9N
Plasmid#80934PurposePlasmid that expresses Cas9N in mammalian cells.DepositorInsertCas9N
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
p(bhsp68-Cas9)
Plasmid#65959PurposeTribolium basal heat shock promoter driving Cas9DepositorInsertCas9
UseCRISPRTagsnuclear localization sequenceExpressionInsectMutationPromoterbhspAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSMVP-Cas9C
Plasmid#80939PurposePlasmid that expresses Cas9C in mammalian cells.DepositorInsertCas9C
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_CEBPB_1
Plasmid#64036PurposeExpresses gRNA against human CEBPB along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX458_CEBPB_2
Plasmid#64047PurposeExpresses gRNA against human CEBPB along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMA-MsgRNA-EGFP
Plasmid#80794PurposeFor insertion of gRNA array containing 11-30 gRNA modulesDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
sg_shuttle_RFP657
Plasmid#134968PurposeLentiviral vector for sgRNA expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6-sgRNA; EFS-RFP657Available sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCD227
Plasmid#113318PurposeAraC-pBAD-dCas9, MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRTagsExpressionBacterialMutationR93APromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-L3US2-RFP-HDAC3
Plasmid#83966PurposeLentiviral CRISPR HDAC3 dual gRNA targeting vectorDepositorInsertHDAC3 dual gRNAs (HDAC3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_pos9_AmpR
Plasmid#119152PurposeEncodes sgRNA targeting position 9 in pColE1_70a_deGFP_KanRDepositorInsertsgRNA targeting position 9 in pColE1_70a_deGFP_KanR
UseCrisprTagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH1v1
Plasmid#60244PurposegRNA expression by the H1 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterH1 promoterAvailable sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMZ283
Plasmid#52224PurposeExpression of sgRNA precursor with CspCI placeholder for target cloning (see comments).DepositorInsertrrk1-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterrrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
PX458_CREB1_2
Plasmid#64940PurposeExpresses gRNA against human CREB1 along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR1002
Plasmid#71820PurposeExpress Streptococcus pyogenes Cas9DepositorInsertT7pr_10xHis-MBP-TEV-SPyCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJF131B
Plasmid#113326PurposepTet-W108 scRNA.b2, pTet-RR2-sgRNADepositorInsertW108 scRNA_MS2 and sgRNA_RR2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB67
Plasmid#47947PurposeExpresses C. elegans optimized Cas9 from the hsp-16.48 promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, and egl-13 NLSExpressionWormMutationPromoterhsp-16.48Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJF104B
Plasmid#113324PurposepTet-dCas9, pTet-MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRTagsExpressionBacterialMutationR93APromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX458_GABPA_2
Plasmid#64255PurposeExpresses gRNA against human GABPA along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMB63
Plasmid#47945PurposeExpresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, and egl-13 NLSExpressionWormMutationPromotereef-1A.1 (eft-3)Available sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
PX458_GABPA_1
Plasmid#64254PurposeExpresses gRNA against human GABPA along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJA50
Plasmid#59931PurposegRNA for cleavage at unc-58(e665) locus in C elegansDepositorInsertunc-58(e665) gRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJA54
Plasmid#60212Purposecleavage near e1350 locus in sqt-1DepositorInsertsqt-1(e1350) gRNA1
UseCRISPRTagsExpressionMutationPromoterU6Available sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_pos3_AmpR
Plasmid#119146PurposeEncodes sgRNA targeting position 3 in pColE1_70a_deGFP_KanRDepositorInsertsgRNA targeting position 3 in pColE1_70a_deGFP_KanR
UseCrisprTagsExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_pos7_AmpR
Plasmid#119150PurposeEncodes sgRNA targeting position 7 in pColE1_70a_deGFP_KanRDepositorInsertsgRNA targeting position 7 in pColE1_70a_deGFP_KanR
UseCrisprTagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_pos5_AmpR
Plasmid#119148PurposeEncodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanRDepositorInsertsgRNA targeting position 5 in pColE1_70a_deGFP_KanR
UseCrisprTagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_pos2_AmpR
Plasmid#119145PurposeEncodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanRDepositorInsertsgRNA targeting position 2 in pColE1_70a_deGFP_KanR
UseCrisprTagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX458_ATF1_1
Plasmid#64690PurposeExpresses gRNA against human ATF1 along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJA55
Plasmid#59935PurposegRNA for cleavage near sqt-1(e1350) locus in C elegansDepositorInsertsqt-1(e1350) gRNA2
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40]
Plasmid#124069PurposeCRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony and Dm-yellow for linearization; (Transformation plasmid; Black eye marker)DepositorInserteb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40
UseCRISPR and Cre/LoxTagsExpressionInsectMutationPromoterbhsp68Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF823_EFS-Cas9-PuroR_lenti
Plasmid#138317PurposeLenti expression of SpyCas9 with Puro selectionDepositorInsertSpyCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAH233
Plasmid#121144PurposeExpression of gRNA with BbsI placeholder for target cloningDepositorInsertgRNA cassette
UseCRISPRTagsExpressionYeastMutationPromoterS.pombe rrk1Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH235
Plasmid#121145PurposeCas9 expression controlled by the nmt41 promoterDepositorInserthumanized Streptococcus pyogenes Cas9
UseCRISPRTagsFlagExpressionYeastMutationPromoternmt41Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEL088
Plasmid#137896PurposeiSTU-CRISPR/Cas12a DR-DR structure crRNA insertion vector with OsCDPK2 intronDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJA14
Plasmid#59929PurposegRNA for cleavage at rde-1(H974) locus in C elegansDepositorInsertrde-1(H974) gRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only