Addgene

We narrowed to 17,685 results for: Met

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pRATIO4212

Plasmid

#141313

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

mScarlet-I and mVenus

Expression

Plant

Mutation

Promoter

UBQ10 promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLIP-puro-dsRed-GFP-miRNA

Plasmid

#32704

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Insert

emGFP-miRNA

Use

Cre/Lox, RNAi, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2011

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNT3/Cre

Plasmid

#89654

Purpose

Expresses a non-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

non-targeting gRNA

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-DsRed-Express

Plasmid

#103995

Purpose

Plasmid encoding DsRed-Express

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

DsRed-Express

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSkipFlox

Plasmid

#85797

Purpose

P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

split Cre recombinase

Use

Cre/Lox; P. falciparum expression

Tags

FKBP, FRB*

Expression

Mutation

Promoter

Pfcrt

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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Tus pGST-//

Plasmid

#53305

Purpose

Expresses GST-tagged E.coli replication terminator protein Tus.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

E.Coli Tus replication terminator protein

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pEB1-mCerulean3

Plasmid

#103973

Purpose

Plasmid encoding mCerulean3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCerulean3

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mVenus ME

Plasmid

#103989

Purpose

Plasmid encoding mVenus ME

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus ME

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Ii-Str_puromycin

Plasmid

#65309

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Use

Tags

Expression

Mammalian

Mutation

Streptavidin in its lumenal part

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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M-ST1-sgRNA

Plasmid

#48672

Purpose

Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

hU

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_VPR

Plasmid

#79376

Purpose

Recruits VPR to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-VPR

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas_5+7_gfp

Plasmid

#60595

Purpose

encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_5+7_gfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCas_2+5

Plasmid

#60594

Purpose

encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_2+5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pMK377 (Hygro-P2A-mCherry2)

Plasmid

#121188

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mCherry2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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paavCAG-JxOFF-pre-mGRASP-mCerulean

Plasmid

#51900

Purpose

JxOFF-pre

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pG-HIV-LRT

Plasmid

#104592

Purpose

Standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

HIV-1 late reverse transcripts

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pIntegrase_7

Plasmid

#60578

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_7

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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mCyRFP1-Cdc42

Plasmid

#84360

Purpose

fusion protein of Cdc42, FRET donor

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mCyRFP1-Cdc42

Use

Tags

mCyRFP1 N terminal fusion to Cdc42

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMito-iRFP720

Plasmid

#45464

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Use

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ906

Plasmid

#44472

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_p65_hsf1

Plasmid

#79374

Purpose

Recruits p65_hsf1 to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-p65-hsf1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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mRuby2-Lifeact-7

Plasmid

#54674

Purpose

Localization: Actin, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Use

Tags

LifeAct-mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pZ8-P_dCas9

Plasmid

#74063

Purpose

pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

dcas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Propionate inducible promoter (prp)

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pJT119b

Plasmid

#50551

Purpose

Expresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Inserts

CcaS
CcaR
sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagRFP-T-Cx43-7

Plasmid

#58009

Purpose

Localization: Gap Junctions, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Cx43

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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ii-Str_SBP-mCherry-GPI

Plasmid

#65297

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEO100c

Plasmid

#50550

Purpose

Expresses sfGFP under the PompC1157 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2014

Availability

Academic Institutions and Nonprofits only
pBS-IT6

Plasmid

#66870

Purpose

Inntag 6: a small self-structured epitope aimed at innocuous protein tagging

Depositor

Marti Aldea

Article

Georgieva et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

Inntag 6

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_DARPinOFF7

Plasmid

#182396

Purpose

Bacterial expression of DARPinOFF7

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

DARPinOFF7

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK408 (mAID-Nluc Hygro)

Plasmid

#121198

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-Nluc Hygro

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2-Katushka

Plasmid

#104008

Purpose

Plasmid encoding Katushka

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Katushka

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (E3V, K6T, N21D, R3…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pIntegrase_5

Plasmid

#60577

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_5

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p1xNLS-FRB-mCherry-hsp86-BSD

Plasmid

#85795

Purpose

Expression of 1xNLS mislocaliser in P. falciparum

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

1xNLS Mislocaliser

Use

P. falciparum expression

Tags

FRB*, mCherry

Expression

Mutation

Promoter

Pfhsp86

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Parp14-c012

Plasmid

#173099

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP14 (PARP14 Human)

Use

Tags

mCerulean

Expression

Bacterial

Mutation

W1003-A1190

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ciCas9_pcDNA5

Plasmid

#100550

Purpose

Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-NTOM20-mVenus-Zdk3

Plasmid

#81012

Purpose

Expression of Zdk1 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk3

Use

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ1655

Plasmid

#51481

Purpose

miniMos transposable element with HygroR, R4-R3 Gateway cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMK004

Plasmid

#34616

Purpose

Viral expression of Arch (D95N mutant with greater sensitivity but slower response) voltage indicator

Depositor

Adam Cohen

Article

Kralj et al Nat Methods. 2011 Nov 27. doi: 10.1038/n

Insert

Arch D95N

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Changed Aspartic Acid 95 to Asparagine

Promoter

CaMKII

Available since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ1663

Plasmid

#51484

Purpose

miniMos transposable element with HygroR and peel-1, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-Talin-N-10

Plasmid

#54266

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Paszek et al Nat Methods. 2012 Jul 1;9(8):825-7. doi:

Insert

Talin

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
CLPIT Cry2PHR-mCh-LRP6c

Plasmid

#42961

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-LRP6c

Use

Retroviral

Tags

Expression

Mammalian

Mutation

LPR6 cytoplasmic domain only

Promoter

Tet-Off promoter

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-StrepII-TagRFP-CohI

Plasmid

#58710

Purpose

Detection construct to label dockerin type I containing fusion proteins

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

TagRFP-CohI

Use

Tags

StrepII

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Parp14-c010

Plasmid

#173100

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARP14 (PARP14 Human)

Use

Tags

mCerulean

Expression

Bacterial

Mutation

F1208-G1388

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-T3G-ERP2

Plasmid

#175188

Purpose

All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRATIO3212

Plasmid

#141311

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

mScarlet-I and mVenus

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrans_9xD3B_Cluc_Norm

Plasmid

#68415

Purpose

CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Cypridina Luciferase-2A-Venus YFP

Use

Luciferase

Tags

Expression

Mammalian

Mutation

YFP: M153T,V163A,S175G ("Venus") YFP Va…

Promoter

minimalCMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRATIO3267

Plasmid

#141312

Purpose

Gateway cloning compatible dual bioluminescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

gLUC and redLUC

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
XW09

Plasmid

#65833

Purpose

Punc-4c-QS-SL2-mcherry3'UTR-unc-54

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Neurospora crassa OR74A repressor (qa-1S)

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Punc-4c

Available since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagRFP-T-Lysosomes-20

Plasmid

#58022

Purpose

Localization: Lysosome Membrane, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

LAMP1

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pSMVP-Cas9FL-P2A-turboGFP

Plasmid

#80941

Purpose

Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9FL

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2351 - 2400 of 17685 results

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