161,152 results
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pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI
Plasmid#115207PurposeExpresses BzoCas13b from a SUMO-tagged bacterial expression constructDepositorInsertBzoCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BSA4-G-L11
Plasmid#127830PurposeProduction of recombinant human ribosomal protein L11DepositorInsertRPL11 (RPL11 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-mRipk3
Plasmid#78830Purposeretroviral expression of mRIPK3DepositorInsertRIPK3 (Ripk3 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoter5'LTRAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCrepusculo
Plasmid#190156PurposeRegulates bacterial expression of target gene in blue-light-repressed manner. Based on Nakamurella multipartita PAL.DepositorTypeEmpty backboneUseTagsHis6 tagExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-FOS
Plasmid#79511PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human FOS CDSDepositorInsertFOS (FOS Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWUR 1+2 tetO mut89
Plasmid#80102PurposeEncodes a set of wild-type Cas1+2 and a mutant set of Cas1+2DepositorUseTagsExpressionBacterialMutationQ24H, P202Q, G241D, E276D, L297QPromoterAvailable sinceDec. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-mutLuc
Plasmid#106458Purposeluciferase reporter, CMV promoter (3 NFkB sites mutated)DepositorInsertluciferase reporter, CMV promoter (3 NFkB sites mutated)
UseLuciferaseTagsExpressionMammalianMutation3 NFkB sites mutatedPromoterAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRC54-DHX37-GFP
Plasmid#192149Purposeexpress DHX37-GFPDepositorInsertDHX37-GFP (Dhx37 Mouse)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-VASP-5
Plasmid#58027PurposeLocalization: Actin/Focal Adhesions, Excitation: 555, Emission: 584DepositorInsertVASP
UseTagsmTagRFPExpressionMammalianMutationA209T in VASPPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI
Plasmid#115220PurposeExpresses Pin3Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin3Cas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI
Plasmid#115210PurposeExpresses AspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertAspCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI
Plasmid#115214PurposeExpresses PauCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPauCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RBM10_p.R171W
Plasmid#81592PurposeGateway Donor vector containing RBM10 , part of the Target Accelerator Plasmid Collection.DepositorInsertRBM10 (RBM10 Human)
UseGateway entry vectorTagsExpressionMutationR171WPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 human HAgHA -plus exon 18a - LONG-Cterm in pcDNA3
Plasmid#198451PurposeN-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a- Long form of alternatively spliced C-teminus, in pcDNA3 vectorDepositorInsertCav2.2 (CACNA1B Human)
UseTagsN-terminal GFP, internal double HAExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 human HAgHA -minus exon 18a -LONG-Cterm in pcDNA3
Plasmid#198914PurposeN-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a - Long form of alternatively spliced C-teminus, in pcDNA3 vectorDepositorInsertCav2.2 (CACNA1B Human)
UseTagsN-terminal GFP, internal double HAExpressionMammalianMutationexon 18a deleted, exon 19 starts 3 nucleotides ea…PromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA1_D262V-FLAG-IRES-eGFP
Plasmid#234620PurposeMammalian expression of full-length hnRNPA1 D262V with C-terminal FLAG tag co-expressing with eGFP via IRES sequenceDepositorInserthnRNPA1 D262V (HNRNPA1 Human)
UseTagsFLAGExpressionMammalianMutationFamillial ALS mutation D262V, C-terminal FLAG tag…PromoterCMVAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEJS2700-CMV-Nme2-ABE8e-nt-E932R
Plasmid#228721PurposeExpresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cellsDepositorInsertN-terminal ABE8e with Nme2 nCas9 (D16A) E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200(1-640)-MBP
Plasmid#203543PurposeFIP200 NTD-GFP mammalian expressionDepositorInsertFIP200 (RB1CC1 Human)
UseTagsMBP-10HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PTPRQ(Trunc1)-COMP5AP-AviTag-9xHis
Plasmid#157614PurposeMammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertPTPRQ (PTPRQ Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-ATG9
Plasmid#188076PurposeRecombinant overexpression of human ATG9 in mammalian cellsDepositorInsertATG9 (ATG9A Human)
UseTagsMBP tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-iRFP-Rab7
Plasmid#174459PurposeExpress iRFP with late endosome-targeting sequence in mammalian cellsDepositorInsertiRFP with late endosome-targeting sequence
UseTagssmall GTPase (Rab7)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#42876PurposeBacterial expression of Cas9 nuclease, tracrRNA and crRNA guideDepositorInserttracr/Cas9
UseCRISPR; E.coliTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-parkin WT
Plasmid#45875DepositorInsertParkin (PRKN Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDule2-pCNF
Plasmid#85495PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
UseTagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)Available sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-BSD
Plasmid#174821PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
UseTagsP2A-BSD, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN2A-Variant-1-IRES-mScarlet
Plasmid#162279PurposeEukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN2A Variant 1, stabilized (SCN2A Human)
UseTagsIRES-mScarletExpressionMammalianMutationIVS intron inserted after bp4551.PromoterCMVAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR
Plasmid#60229PurposeExpresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Cre recombinase
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HAExpressionMammalianMutationPromoterCBh and U6Available sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXR003: CasRx gRNA cloning backbone
Plasmid#109053PurposehU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterA. nidulans gpdA promoter with Hammerhead ribozym…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP-Nucleus-7
Plasmid#55265PurposeLocalization: Nuclear Localization, Excitation: 399, Emission: 456DepositorInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
UseTagsmTagBFP-NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pICSL11024 (pICH47732::NOSp-NPTII-OCST)
Plasmid#51144PurposeConfers KAN resistance in plantaDepositorInsertNOSp-NPTII-OCST
UseTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-(-)-Pur
Plasmid#200458PurposeEukaryotic expression vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-Hylight-T152E
Plasmid#193448PurposeA binding-dead fluorescent biosensor for fructose 1,6-bisphosphate (FBP) for negative control experiments (mammalian expression vector)DepositorInsertBinding-Dead Hylight
UseTagsExpressionMammalianMutationThreonine 67 (ACT) mutated to a Glutamic Acid (GA…PromoterCMVAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-RiboL1-jGCaMP8m
Plasmid#192617PurposeAAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.DepositorInsertFLEX-RiboL1-jGCaMP8m
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-Tmem192-2xFlag
Plasmid#102929PurposeExpress a lysosomal TagDepositorInsertTmem192 (TMEM192 Human)
UseLentiviralTagsFlagExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AMPKAR
Plasmid#192446PurposeGenetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.DepositorInsertExRai-AMPKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCBL101-RUBY
Plasmid#199723PurposeExpresses betalain biosynthesis genes under CaMV 35S promoterDepositorInsert35S-RUBY
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GNSTM-3-Flag-10-Lamp2b-HA
Plasmid#71293PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsFLAG, GNSTM glycosylation motif, HA, and Lamp2 si…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-hTERT
Plasmid#1773PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pBpF
Plasmid#31190PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.DepositorInsertM.j. p-benzoylphenylalanine RS (2 copies +tRNA)
UseTagsExpressionBacterialMutationY32G, E107P, D158T, I159SPromoterAvailable sinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCI HA NEDD4L
Plasmid#27000DepositorInsertNEDD4L (NEDD4L Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCasSA
Plasmid#98211PurposeCRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV_miniENVLPE
Plasmid#232431PurposeExpression plasmid to produce miniENVLPE particlesDepositorInsertminiENVLPE
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-miRFP670nano3
Plasmid#184664PurposeMonomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmidDepositorInsertmiRFP670nano3
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
mPiezo1-IRES-eGFP
Plasmid#80925Purposeexpresses mouse Piezo1 with GFPDepositorInsertmouse Piezo1 CDS (Piezo1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR
Plasmid#126679PurposeFluorescent reporter for cyclin E/A-CDK and CDK4/6 activitiesDepositorInsertRb (a.a 886-928)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-NEMOm
Plasmid#189930PurposeMammalian expression of calcium sensor with ultra-high dynamics and sensitivityDepositorInsertNEMOs
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
HCT47_pAAV-eHGT_3Xcore_1137m-minBGprom-iCre297T-WPRE3-bGHpA
Plasmid#233564PurposeDirect-expressing SYFP2 AAV virusInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBglobinAvailable sinceJune 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-G3BP1-WT
Plasmid#135997PurposeWT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cellsDepositorInsertG3BP1 (G3BP1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only
