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We narrowed to 4,232 results for: Gca

Showing: 2401 - 2450 of 4232 results

  1. CMV-GEM-GECO1

    Plasmid
    #32442
    Purpose
    Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    GEM-GECO1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32442-plasmid-map-sequence-id-117095

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  2. gRNA NIA TLS1

    Plasmid
    #196980
    Purpose
    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.
    Depositor
    Friedrich Kragler
    Article
    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    AtNIA1 gRNAs fused to TLS1 tags
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pU6-26, pU6-29
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196980-plasmid-map-sequence-id-390456

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  5. pRDA_936

    Plasmid
    #216094
    Purpose
    Cas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive control
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Insert
    CD46, CD47, CD55, CD81 guides
    Use
    CRISPR and Lentiviral; Positive controls
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216094-plasmid-map-sequence-id-431378

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  8. pCRISPEY-GAL-Nat-ADE2

    Plasmid
    #232103
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232103-plasmid-map-sequence-id-461835

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  12. Rescue Septin 11 mRNA

    Plasmid
    #122022
    Purpose
    Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.
    Depositor
    Angel de Blas
    Article
    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6
    Insert
    SEPT11 (Sept11 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Silent mutations are in 5 consecutive codons (AUU…
    Promoter
    Available since
    Feb. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122022-plasmid-map-sequence-id-235846

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  15. ATP1A1_G3_Dual_sgRNA

    Plasmid
    #173202
    Purpose
    Coselection for PE3 or HDR in human cells. Vector for tandem expression of ATP1A1 G3 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173202-plasmid-map-sequence-id-339519

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  16. LLP773_pLenti-6xHRBKET-sgRNA

    Plasmid
    #211766
    Purpose
    Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    pCMV and U6
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211766-plasmid-map-sequence-id-417396

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  20. pX335-EN477

    Plasmid
    #86232
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    86232-plasmid-map-sequence-id-177242

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  29. CMV-GEX-GECO1

    Plasmid
    #32443
    Purpose
    Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    GEX-GECO1.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  35. pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp

    Plasmid
    #101349
    Purpose
    2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3
    Depositor
    Jeremy Luban
    Article
    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:
    Insert
    NL4-3
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    delta tat delta TAR delta env GFP in Nef Tet ope…
    Promoter
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    101349-plasmid-map-sequence-id-194312

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  47. pSCL.175

    Plasmid
    #184996
    Purpose
    Express -Eco1 HEK3 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Use
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    HEK3 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184996-plasmid-map-sequence-id-376621

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  48. pmIL-6 mut C/EBP

    Plasmid
    #61291
    Purpose
    drives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) site
    Depositor
    Gail Bishop
    Article
    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.
    Insert
    IL-6 promoter (Il6 Mouse)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Mutated C/EBP binding site from ACATTGTGCAATCT to…
    Promoter
    IL-6 promoter with mutant C/EBP binding site
    Available since
    May 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  49. pSCL.177

    Plasmid
    #184998
    Purpose
    Express -Eco1 EMX1 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Use
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    EMX1 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184998-plasmid-map-sequence-id-376623

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Showing: 2401 - 2450 of 4232 results