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Plasmid#60859Purpose2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoterDepositorInsertDLX1 (DLX1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGPA1
Plasmid#115476PurposeGeneration of mariner-based transposon mutant libraries in Enterococcus faeciumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
CBE-T1.46
Plasmid#193284PurposeExpression of CBE-T1.46 base editor in mammalian cells, CMV promoterDepositorInsertCBE-T1.46
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcsII-pb5-flag
Plasmid#86769PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertproteasome beta subunit 5 (PSMB5 Human)
UseLentiviralTagsflagExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shSTING-Hsa
Plasmid#128158PurposeDoxycyclin inducible shRNA knockdown of human STING geneDepositorInsertshSTING Hsa (STING1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Hb9-GFP
Plasmid#37080Purpose3rd generation lentiviral transfer plasmid for Hb9 GFPDepositorInsertHb9 GFP
UseLentiviralTagsExpressionMutationPromoterHb9Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTriExRhoA2G
Plasmid#40176DepositorInsertRhoA Biosensor
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmRFP_a_tubulin_IRES_puro2b
Plasmid#21041DepositorInsertalpha Tubulin (TUBA1B Human)
UseTagspmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2B tdTomato
Plasmid#116870PurposeNuclear localized tdTomatoDepositorInsertH2B tdTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-eSpCas9(1.1)
Plasmid#108292PurposepX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutationsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-ASAP1
Plasmid#52519PurposeFor mammalian expression of the fluorescent voltage sensor ASAP1DepositorInsertAccelerated Sensor of Action Potentials 1
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRE-tdTomato
Plasmid#50798PurposeExpression of tdTomato under the control of the TRE-promoterDepositorInsertTREtight driven tdTomato fluorescent protein
UseMutli-speciesTagsExpressionMutationPromoterTREtightAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKMyc
Plasmid#19400DepositorTypeEmpty backboneUseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRRL SFFV d20GFP.T2A.mTagBFP Donor
Plasmid#31485DepositorInsertSFFV d20BFP T2A BFP
UseLentiviralTagsExpressionMutationGFP has 20 amino acids deleted from the C terminu…PromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-tetR
Plasmid#62561PurposeTetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP4B_LAP
Plasmid#101850PurposeTet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCharged Multivesicular Body Protein 4B (CHMP4B Human)
UseGateway cloning, flp-frt recombination, tet-induc…TagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
alpha Synuclein-VC
Plasmid#89471Purposeexpresses human alpha Synuclein fused to the C-terminal part of VenusDepositorInsertSNCA (SNCA Human)
UseTagsC-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet1 CD
Plasmid#70129PurposeExpress flag-tagged mouse Tet1 catalytic domain.DepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET1 catalytic domain.PromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGSKU
Plasmid#72243PurposeContains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
kanMX4
I-SceI
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Foxo4
Plasmid#17549DepositorInsertFoxO4 (FOXO4 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
p6-8
Plasmid#118089Purposerapa-T7- mRNA(GFP)DepositorInsertRapamycin-inducible split T7 RNAP with T7 driven mRNA for GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-p53DD
Plasmid#22729DepositorInsertTrp53 (Trp53 Mouse)
UseRetroviralTagsExpressionMammalianMutationdeleted amino acids 14-301PromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJH376
Plasmid#86842Purposemamalian expression of anti-CRISPR protein AcrIIA4DepositorInsertacrIIA4
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-eYFP
Plasmid#104056PurposeAn AAV genome with tet-inducible expression of the fluorescent protein eYFPDepositorInsertEYFP
UseAAVTagsExpressionMutationPromoterTREAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pTriExEKAR2G2
Plasmid#40175DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3XFLAG-wtGATA6-3XAU1 neo
Plasmid#72618PurposeExpresses an inducible, N-terminally 3X-FLAG tagged and C-terminally 3X-AU1 tagged wtGATA6DepositorInsertGATA binding protein 6 (GATA6 Human)
UseLentiviralTags3XAU1 and 3XFLAGExpressionMammalianMutationPromoterTRE TightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV4Neo-murine IL-17RA-HA
Plasmid#46859Purposeexpresses mouse IL-17RADepositorInsertIL-17RA (Il17ra Mouse)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro-NMYC TRF2
Plasmid#16348DepositorInserthTRF2 (TERF2 Human)
UseRetroviralTagsMYCExpressionMammalianMutationaa2-500PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
mCherry PP1 gamma
Plasmid#45220DepositorInsertmCherry PP1 gamma
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
psPAX2-D64V
Plasmid#63586PurposepsPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.DepositorInsertPackaging plasmid for integrase deficient lentiviral vectors
UseIntegrase deficient lentiviral packaging vectorTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-D4H*-mCherry
Plasmid#134604Purposefluorescent cholesterol-binding probeDepositorInsertD4H*
UseTagsGST and mCherryExpressionBacterialMutationY415A and A463WPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF6-CSPG4-myc-his
Plasmid#69037PurposeExpression of CSPG4 in mammalian cellsDepositorInsertCSPG4 (CSPG4 Human)
UseTagsHis and MycExpressionMammalianMutationnonePromoterEF-1 alphaAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/5
Plasmid#104964PurposeAAV packaging plasmid expressing Rep/Cap genes for production of serotype 5DepositorInsertRep2/Cap5
UseAAVTagsExpressionMammalianMutationPromoterp5Available sinceOct. 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pLV-mCherry
Plasmid#36084Purpose3rd generation lentiviral plasmid expressing mCherryDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-dCas9-VP64-6xHis
Plasmid#62935PurposeExpression of dCas9-VP64-6xHis in bacterial cellsDepositorInsertdCas9-VP64
UseTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A and H840A amino acid changes render Cas9 nuc…PromoterT7Available sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
scramble shRNA
Plasmid#1864Purpose3rd gen lentiviral negative control vector containing scrambled shRNADepositorInsertscramble
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTargetF
Plasmid#62226PurposeConstitutive expression of sgRNA without donor editing template DNADepositorInsertsgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpij23119Available sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSin Helper
Plasmid#127694PurposePlasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.DepositorInsertSindbis Glycoproteins
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRK5-HA-Ubiquitin-K48
Plasmid#17605PurposeMammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK48 only, other lysines mutated to arginines. Enh…PromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEcgRNA
Plasmid#166581PurposeCRISPR-Cas9-assisted genome editing in Escherichia coliDepositorInsertccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgRNA
Plasmid#124844PurposeVector for Cre-dependent expression of SaCas9DepositorInsertSaCas9, gRNA scaffold
UseAAV, CRISPR, and Mouse TargetingTagsNLS and NLS-3xHAExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)
Plasmid#129405Purposecircular Pepper RNADepositorInsertcircular F30-Pepper(TAR Variant-2)
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXL010-Wnt dual (GFP-Fire) reporter
Plasmid#40588DepositorInsertWnt reporter sequences
UseLentiviralTagsExpressionMammalianMutationPromoterWnt promoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP
Plasmid#102796PurposeRetrovirus for delivery of one sgRNA (empty, bbsI)DepositorInsertU6 sgRNA
UseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF
Plasmid#31186PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAGDepositorInsertM.j. p-azidohenylalanine RS (2 copies +tRNA)
UseTagsExpressionBacterialMutationY32T, E107N, D158P, I159L, L162Q, D286RPromoterAvailable sinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mGreenLantern
Plasmid#161912PurposemGreenLantern fluorescent protein: cytosolic expression in mammalian cellsDepositorInsertmGreenLantern
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA
Plasmid#128034PurposeMammalian expression vector with N terminal HA tagDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304_CMV::mCherry-LgBiT-FLAG-NES
Plasmid#199715PurposeExpresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.DepositorInsertmCherry-LgBiT-FLAG-NES
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only