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Showing: 2751 - 2800 of 4064 results

  10. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

    Plasmid
    #71707
    Purpose
    A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid
    Depositor
    Giulio Draetta
    Article
    Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16
    Insert
    humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
    Use
    CRISPR
    Tags
    3xFLAG and hGEM(1/110)
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    71707-plasmid-map-sequence-id-424083

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  12. pAnc80L65

    Plasmid
    #68837
    Purpose
    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details
    Depositor
    Luk Vandenberghe
    Article
    Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1
    Insert
    Ancestral AAV Capsid Anc80L65
    Use
    AAV and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Rep2
    Available since
    Nov. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    68837-plasmid-map-sequence-id-121219

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  14. AIO-mCherry

    Plasmid
    #74120
    Purpose
    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing
    Depositor
    Steve Jackson
    Article
    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    74120-plasmid-map-sequence-id-275140

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  32. pEF1a-FB-dCas9-puro

    Plasmid
    #100547
    Purpose
    Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein
    Depositor
    Jian Xu
    Article
    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.
    Inserts
    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
    puromycin
    Use
    CRISPR
    Tags
    FLAG, Biotin-acceptor-site (Avitag)
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1a
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    100547-plasmid-map-sequence-id-313417

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  33. pCMV-SHP2 WT

    Plasmid
    #8381
    Depositor
    Ben Neel
    Insert
    SHP2 (PTPN11 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 15, 2005
    Availability
    Academic Institutions and Nonprofits only
    8381-plasmid-map-sequence-id-363016

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  40. pLenti CMV/TO GFP-MDC1 (779-2)

    Plasmid
    #26285
    Depositor
    Eric Campeau
    Insert
    Mediator of DNA damage checkpoint 1 (MDC1 Human)
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 25, 2010
    Availability
    Academic Institutions and Nonprofits only
    26285-plasmid-map-sequence-id-228307

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  41. pTP1005

    Plasmid
    #104160
    Purpose
    Bacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
    Use
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104160-plasmid-map-sequence-id-200057

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  44. pBabe-Puro-BRAF-V600E

    Plasmid
    #15269
    Purpose
    Retroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)
    Depositor
    William Hahn
    Article
    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.
    Insert
    BRAF (BRAF Human)
    Use
    Retroviral
    Tags
    His and Myc
    Expression
    Mammalian
    Mutation
    Valine 600 to Glutamate (mutation has been found …
    Promoter
    Available since
    July 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    15269-plasmid-map-sequence-id-217979

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Showing: 2751 - 2800 of 4064 results