Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

20+ requests Clear filter

Applied Filters

20+ requests Clear filter

19,770 results

Showing: 3201 - 3250 of 19770 results

  2. pICE-HA-NLS-I-PpoI

    Plasmid
    #46963
    Purpose
    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    NLS-I-PpoI
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV-TET
    Available Since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46963-plasmid-map-sequence-id-66854

    Enlarge

  4. pDEST-CMV mCherry-GFP-LC3B WT

    Plasmid
    #123230
    Purpose
    Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    123230-plasmid-map-sequence-id-244035

    Enlarge

  5. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid
    #133451
    Purpose
    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection
    Depositor
    Mark Howarth
    Article
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Insert
    TfR-sfGFP-myc tag-SpyCatcher003
    Tags
    GSSGS and myc tag
    Expression
    Mammalian
    Mutation
    Contains C20 and A23 mutations that improve plasm…
    Promoter
    CMV
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133451-plasmid-map-sequence-id-267048

    Enlarge

  34. Pet28a_Synthetic_Human_H2AZ.1

    Plasmid
    #42629
    Depositor
    Joe Landry
    Insert
    Human H2AZ.1 (H2AZ1 Codon Optimized, Human, Synthetic)
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Codon Optimized
    Promoter
    T7
    Available Since
    March 21, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42629-plasmid-map-sequence-id-434040

    Enlarge

  41. CMV-mito-GEM-GECO1

    Plasmid
    #32461
    Purpose
    Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    GEM-GECO
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    CMV
    Available Since
    Dec. 5, 2011
    Availability
    Academic Institutions and Nonprofits only
    32461-plasmid-map-sequence-id-123705

    Enlarge

  47. pLV-EF1-Cre-PGK-Puro

    Plasmid
    #108543
    Purpose
    Lentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoter
    Depositor
    Javier Alcudia
    Inserts
    cre recombinase
    Puromycin
    Promoter
    EF1apha and PGK
    Available Since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    108543-plasmid-map-sequence-id-208672

    Enlarge

Showing: 3201 - 3250 of 19770 results