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159,258 results

Showing: 3301 - 3350 of 159258 results

  1. TFORF0846

    Plasmid
    #141732
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    YBX1 (YBX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141732-plasmid-map-sequence-id-404379

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  2. pCSC-LHX8-IRES-GBX1

    Plasmid
    #182235
    Purpose
    To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with ASCL1, Sox11, FGF2 and two small molecules, forskolin and LDN-193189
    Depositor
    Chun-Li Zhang
    Article
    Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.
    Insert
    LHX8-IRES-GBX1 (LHX8, GBX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    March 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    182235-plasmid-map-sequence-id-357464

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  4. qTAG-N-Puro-mNeon-ACTB

    Plasmid
    #207752
    Purpose
    Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    207752-plasmid-map-sequence-id-414897

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  5. pET-29b(+)_His-CBD-SpEndoH

    Plasmid
    #218196
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH
    Depositor
    Florence Pojer
    Insert
    EndoH
    Use
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-313
    Promoter
    T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218196-plasmid-map-sequence-id-431799

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  8. pSDMA58

    Plasmid
    #67942
    Purpose
    Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains HYG resistance marker.
    Depositor
    James Fraser
    Article
    Arras et al PLoS One. 2015 Apr 9;10(4):e0122916. doi
    Type
    Empty backbone
    Use
    Cryptococcal complementation vector
    Tags
    Expression
    Mutation
    Promoter
    ACT1 for Cryptococcal marker
    Available since
    Oct. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    67942-plasmid-map-sequence-id-120524

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  14. pJMP3665

    Plasmid
    #222351
    Purpose
    Expression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.
    Depositor
    Jason Peters
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222351-plasmid-map-sequence-id-439073

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  17. pLenti-basP:fLuc-TK:rLuc

    Plasmid
    #138369
    Purpose
    Lentiviral plasmid contains basal promoter driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter
    Depositor
    Nathan Lawson
    Article
    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC
    Insert
    sequence multi cloning sites, minP driving firefly luciferase and TK promoter driving renilla luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    138369-plasmid-map-sequence-id-272648

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  22. pCoofy55

    Plasmid
    #122007
    Purpose
    Mammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag options
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    His10-SumoStar (Life Sensors) and Multi2: dependi…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    122007-plasmid-map-sequence-id-243400

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  24. pUS212

    Plasmid
    #84580
    Purpose
    E.coli cloning and expression vector. Has cloning sites downstream of Tet promoter.
    Depositor
    Nicholas Coleman
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 21, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    84580-plasmid-map-sequence-id-166638

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  30. pJMP3664

    Plasmid
    #222350
    Purpose
    Expression vector with PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.
    Depositor
    Jason Peters
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222350-plasmid-map-sequence-id-439070

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  33. pSDMA25

    Plasmid
    #67940
    Purpose
    Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains nourseothricin resistance marker (NAT)
    Depositor
    James Fraser
    Article
    Arras et al PLoS One. 2015 Apr 9;10(4):e0122916. doi
    Type
    Empty backbone
    Use
    Cryptococcal complementation vector
    Tags
    Expression
    Mutation
    Promoter
    ACT1 for Cryptococcal marker
    Available since
    Oct. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    67940-plasmid-map-sequence-id-120525

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  35. pJBL703

    Plasmid
    #140373
    Purpose
    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoter
    Depositor
    Julius Lucks
    Article
    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038
    Insert
    T7-2BP-tetO-3WJdB-T
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140373-plasmid-map-sequence-id-297839

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  36. TFORF1662

    Plasmid
    #142368
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ARID5B (ARID5B Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    142368-plasmid-map-sequence-id-454974

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  39. qTAG-N-Puro-moxGFP-LMNB1

    Plasmid
    #207773
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207773-plasmid-map-sequence-id-413797

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  43. pET-29b(+)_His-CBD-3C-EmPNGaseF

    Plasmid
    #218193
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF
    Depositor
    Florence Pojer
    Insert
    PNGaseF
    Use
    Tags
    tufA-His-CBD-3C
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 41-354
    Promoter
    T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218193-plasmid-map-sequence-id-431798

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Showing: 3301 - 3350 of 159258 results