165,688 results

1. pEF1a-2xSV40_NLS-NLuc

   Plasmid

   #135953

   Purpose

   Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Tags

   2xSV40-NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2061

   Plasmid

   #141900

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pT3-myr-AKT-HA

   Plasmid

   #31789

   Depositor

   Xin Chen

   Article

   Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

   Insert

   Akt1 (Akt1 Mouse)
   

   Tags

   HA and Myr

   Expression

   Mammalian

   Available Since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221_SLC9A9

   Plasmid

   #132211

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC9A9 (SLC9A9 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 28, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pcDNA3-EGFP-RhoA-T19N

   Plasmid

   #12967

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   RhoA DN (RHOA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T19N Dominant negative

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMBP-AsCas12a

   Plasmid

   #113430

   Purpose

   Expresses 10xHis-MBP fused to AsCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   AsCas12a
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-PGK-EGFP-ALDH1A3-Neo

   Plasmid

   #189744

   Purpose

   The construct was used to express GFP tagged human ALDH1A3 in mammalian cells for the analysis of the subcellular location of this protein.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A3 (ALDH1A3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lentiviral mBmal1-Luc

   Plasmid

   #182762

   Purpose

   Lentiviral vector encoding the promoter region of mouse Bmal1 gene driving a luciferase reporter

   Depositor

   Qing-Jun Meng

   Article

   Gossan et al Arthritis Rheum. 2013 Sep;65(9):2334-45.

   Insert

   mouse arntl promoter (Bmal1 Mouse)
   

   Use

   Lentiviral

   Promoter

   649 bp

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBPZpGAL4DBDUw

   Plasmid

   #26233

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   Zip-GAL4DBD
   

   Expression

   Insect

   Available Since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-ATG2C(VPS13C)

    Plasmid

    #232868

    Purpose

    Expresses the ATG2C domain of VPS13C in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

    Insert

    the ATG2C domain of VPS13C (VPS13C Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKT0178

    Plasmid

    #8744

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    tdimer2
    

    Tags

    Codon optimized linker

    Expression

    Yeast

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG416Gal-GFP

    Plasmid

    #38309

    Depositor

    Susan Lindquist

    Article

    McLellan et al ACS Chem Biol. 2012 Jun 22.

    Insert

    EGFP (eGFP )
    

    Expression

    Yeast

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

13. UBC1cer120G

    Plasmid

    #161017

    Purpose

    ubiquitously weakly expressed 120-meric GFP nanocage

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    120meric GFP nanocage
    

    Expression

    Plant

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG416GAL-ccdB-EYFP

    Plasmid

    #14219

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFUW-tetO-Irf4

    Plasmid

    #139804

    Purpose

    doxycycline-inducible expression of mouse Irf4 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Irf4 (Irf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMAT11

    Plasmid

    #112592

    Purpose

    Used for expression of protein in E. coli as Thrombin and TEV cleavableN-terminal 6His-tag-MBP fusions and without NcoI site within MBP.

    Depositor

    Johan Peränen, Marko Hyvönen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His-MBP

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-smURFP-P2A-GFP

    Plasmid

    #197151

    Purpose

    Expresses the protein of smURFP-P2A-GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    smURFP-P2A-GFP
    

    Use

    AAV

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. HSP70-pp7i-mCh-UPG

    Plasmid

    #161011

    Purpose

    C-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PP7 and H2B-mScarlet
    

    Expression

    Plant

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. iLOV-Farnesyl-5

    Plasmid

    #56516

    Purpose

    Localization: Membrane, Excitation: 450, Emission: 495

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    iLOV

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pcDNA3 APEX2-NES

    Plasmid

    #49386

    Purpose

    Expresses APEX2 in the cytosol of mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Flag - APEX2 - NES
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available Since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAd388-shLuc

    Plasmid

    #83275

    Purpose

    shRNA targeting luciferace

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA to Luciferase
    

    Use

    Retroviral

    Promoter

    pUC ori

    Available Since

    Dec. 2, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pAAV9-null

    Plasmid

    #200182

    Purpose

    Expresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 site

    Depositor

    Aravind Asokan

    Article

    Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

    Insert

    AAV9 Cap w/ nonsense mutation at VR8
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Replaced amino acids 586-592 in Cap with nonsense…

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJC162.1_pc2RNAi

    Plasmid

    #26541

    Depositor

    Phillip Newmark

    Article

    Collins et al PLoS Biol. 2010 . 8(10):e1000509.

    Insert

    prohormone convertase 2
    

    Use

    RNAi

    Available Since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

24. Str-Ii_VSVG-SBP-mCherry

    Plasmid

    #65301

    Purpose

    synchronize trafficking of VSVG from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI

    Plasmid

    #246755

    Purpose

    RNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BsaI.

    Depositor

    Wensheng Wei

    Article

    Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

    Insert

    CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI
    

    Use

    Synthetic Biology

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEM-T_CDC45-HaloTag

    Plasmid

    #244244

    Purpose

    CRISPR/Cas9 donor plasmid to tag human CDC45 with Halo tag

    Depositor

    Kazuhiro Maeshima

    Article

    Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

    Insert

    CDC45 (CDC45 Human)
    

    Use

    CRISPR

    Tags

    HaloTag

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CIRC-V1-Azo-CVB3 IRES-EGFP-BbsI

    Plasmid

    #246757

    Purpose

    RNA circularization with CIRC using Azo intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.

    Depositor

    Wensheng Wei

    Article

    Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

    Insert

    CIRC-V1-Azo-CVB3 IRES-EGFP-BbsI
    

    Use

    Synthetic Biology

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PBAN-tetOn-NKX3.1

    Plasmid

    #238472

    Purpose

    Doxycycline inducible expression of the NKX3.1 transcription factor for mural cell differentiation.

    Depositor

    Juan Melero-Martin

    Article

    Lee et al Nat Commun. 2024 Sep 30;15(1):8392. doi:

    Insert

    NKX3.1 (NKX3-1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX330_CDC45

    Plasmid

    #244243

    Purpose

    Expresses SpCas9 and a sgRNA targeting the human CDC45 loci for knock-in.

    Depositor

    Kazuhiro Maeshima

    Article

    Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

    Insert

    sgRNA targeting human CDC45 (CDC45 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NanoBRET Assay Vector, DAP12-NanoLuc

    Plasmid

    #238723

    Purpose

    Express DAP12-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    DAP12 (TYROBP Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. NanoBRET Assay Vector, TREM1-HaloTag

    Plasmid

    #238718

    Purpose

    Express TREM1-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    TREM1 (TREM1 Human)
    

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. HaloTag-TREM2 Vector

    Plasmid

    #238682

    Purpose

    Express HaloTag-TREM2 Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    TREM2 (TREM2 Human)
    

    Tags

    HaloTag (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pFUW-TetO-Runx2

    Plasmid

    #207225

    Purpose

    doxycycline-inducible expression of Mouse Runx2 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

    Insert

    pFUW-TetO-RUNX2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGY208

    Plasmid

    #239803

    Purpose

    Express mCherry in filamentous fungi

    Depositor

    Kyle Pomraning

    Article

    Guoliang et al ACS Synthetic Biology Article ASAP

    Insert

    mCherry
    

    Use

    Fungal expression

    Promoter

    Tef1IN

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAVEC1_C-TurboID

    Plasmid

    #231285

    Purpose

    For stably expressing fusion proteins with C terminal TurboID for proximity labeling in plants

    Depositor

    Jacob Brunkard

    Article

    Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

    Type

    Empty backbone

    Tags

    TurboID

    Expression

    Plant

    Mutation

    N/A

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGL4 [luc2P/STAT4-RE/Hygro] Vector

    Plasmid

    #236820

    Purpose

    Express STAT4 response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    STAT4 (STAT4 Human)
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    minP

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pGL4 [luc2P/hID1/Hygro] Vector

    Plasmid

    #236808

    Purpose

    Express hID1 response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    hID1 (C17orf28 Human)
    

    Use

    Luciferase

    Mutation

    None

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. AiP13569 - pAAV-AiE0387m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3569)

    Plasmid

    #230459

    Purpose

    AiE0387m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Jonathan Ting, Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAVS1 EGFP-p62 HRD

    Plasmid

    #207550

    Purpose

    Homologous recombination donor to integrate and expression cassette for eGFP-p62 in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible EGFP-P62
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAG Lenti CMV HA-EGFP Puro

    Plasmid

    #236081

    Purpose

    Lentiviral backbone expressing N-terminally HA tagged EGFP, control for empty backbone 236079

    Depositor

    Melina Fan

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV

    Available Since

    April 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. p413 TEF NAPstar1

    Plasmid

    #232738

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar1 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar1
    

    Expression

    Yeast

    Available Since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pXJ-Myc-TCF7L2

    Plasmid

    #232644

    Purpose

    transient expresses TCF7L2 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    TCF7L2 (TCF7L2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. lvl0-G-CasE

    Plasmid

    #229184

    Purpose

    lvl 0 5 gene

    Depositor

    Ron Weiss

    Article

    Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

    Insert

    CasE
    

    Use

    Synthetic Biology

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRSET A-mEosEM-E

    Plasmid

    #226336

    Purpose

    Photoswitchable fluorescent protein for correlative light-electron microscopy; mEosEM-E for prokaryotic expression

    Depositor

    Pingyong Xu

    Article

    Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

    Insert

    mEosEM-E
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pcDNA5-FRT/TO-eGFP-msMMS22L

    Plasmid

    #221014

    Purpose

    mammalian expression vector of eGFP tagged mouse MMS22L

    Depositor

    Daniel Durocher

    Article

    O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

    Insert

    MMS22L (Mms22l Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    naturally siResistant to siMMS22L (human) aagacuu…

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. AAVS1 EGFP-LC3B HRD

    Plasmid

    #207551

    Purpose

    Homologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible EGFP-LC3B
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. AAVS1 EGFP-GABARAPL1 HRD

    Plasmid

    #207552

    Purpose

    Homologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible EGFP-GABARAPL1
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pMS77

    Plasmid

    #215681

    Purpose

    Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
    

    Use

    CRISPR

    Expression

    Worm

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid

    #174901

    Purpose

    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG and NES

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pJUMP24_T24_pRham-ilvA-relE

    Plasmid

    #201531

    Purpose

    Expressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Dan Park

    Article

    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

    Insert

    ilvA-relE
    

    Use

    Synthetic Biology

    Promoter

    PrhaBAD (rhamnose)

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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