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161,273 results

Showing: 3351 - 3400 of 161273 results

  2. TFORF1983

    Plasmid
    #143562
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TBX5 (TBX5 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    143562-plasmid-map-sequence-id-385812

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  4. CMV-Hylight-T152E

    Plasmid
    #193448
    Purpose
    A binding-dead fluorescent biosensor for fructose 1,6-bisphosphate (FBP) for negative control experiments (mammalian expression vector)
    Depositor
    Richard Goodman
    Article
    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119
    Insert
    Binding-Dead Hylight
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Threonine 67 (ACT) mutated to a Glutamic Acid (GA…
    Promoter
    CMV
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    193448-plasmid-map-sequence-id-378448

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  11. pYPQAT57

    Plasmid
    #223429
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223429-plasmid-map-sequence-id-453502

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  13. pAAVone-AAV9-CMV-eGFP

    Plasmid
    #230928
    Purpose
    pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.
    Depositor
    Qizhao Wang
    Insert
    eGFP
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230928-plasmid-map-sequence-id-458358

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  14. pLV6-Bmal-luc

    Plasmid
    #68833
    Purpose
    Expression of Bmal1-luc in mammalian cells
    Depositor
    Steven Brown
    Insert
    Bmal1 regulatory sequences (Bmal1 Mouse)
    Use
    Lentiviral and Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    68833-plasmid-map-sequence-id-455401

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  21. pKL2299A

    Plasmid
    #222105
    Purpose
    A ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.
    Depositor
    Kan Wang
    Article
    Aliu et al Front Plant Sci. 2024 Jul 23;15:1429353.
    Insert
    virA
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    virA promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    222105-plasmid-map-sequence-id-442317

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  26. pS148∙CsR

    Plasmid
    #197858
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Article
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Insert
    gRNA scaffold - Ap/Cb resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197858-plasmid-map-sequence-id-393255

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  28. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid
    #129593
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    Use
    Tags
    6xHis-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129593-plasmid-map-sequence-id-254271

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  32. pPP085

    Plasmid
    #158795
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158795-plasmid-map-sequence-id-309510

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  33. CROPSeq-BFP

    Plasmid
    #196718
    Purpose
    CROP-Seq library plasmid with Chen tracrRNA (PMID: 24360272) and TagBFP
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196718-plasmid-map-sequence-id-388068

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  42. pZac2.1-GfaABC1D-lck-jGCaMP8f

    Plasmid
    #176759
    Purpose
    AAV-mediated expression of membrane bound GCaMP8f under the control of GfaABC1D promoter
    Depositor
    Loren Looger
    Has Service
    AAV1 and AAV9
    Insert
    lck-jGCaMP8f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    GfaABC1D
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    176759-plasmid-map-sequence-id-349056

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  47. TFORF0500

    Plasmid
    #143709
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PPARGC1A (PPARGC1A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    143709-plasmid-map-sequence-id-409093

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Showing: 3351 - 3400 of 161273 results