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4,124 results

Showing: 241 - 280 of 4124 results

  1. LentiCRISPRv2-mCherry

    Plasmid
    #99154
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Depositor
    Agata Smogorzewska
    Inserts
    Cas9
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    99154-plasmid-map-sequence-id-352449

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  2. pcDNA3.1-HA

    Plasmid
    #128034
    Purpose
    Mammalian expression vector with N terminal HA tag
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    128034-plasmid-map-sequence-id-422971

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  3. pComb3XTT

    Plasmid
    #63891
    Purpose
    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)
    Depositor
    Carlos Barbas
    Article
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Insert
    TT Fab
    Use
    Phage display
    Tags
    6xHis, HA tag, and geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63891-plasmid-map-sequence-id-450336

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  5. rtTA-N144

    Plasmid
    #66810
    Purpose
    2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance
    Depositor
    Andrew Yoo
    Article
    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi
    Insert
    Reverse tetracycline-controlled transactivator
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1a
    Available since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    66810-plasmid-map-sequence-id-418923

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  10. pTight-9-124-BclxL

    Plasmid
    #60857
    Purpose
    2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter
    Depositor
    Andrew Yoo
    Article
    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1
    Insert
    miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pTight
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60857-plasmid-map-sequence-id-195309

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  11. pCDH-EF1-mVenus-p27K−

    Plasmid
    #176651
    Purpose
    It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).
    Depositor
    Mohamed Bentires-Alj
    Article
    Correia et al Nature. 2021 Jun;594(7864):566-571. doi:
    Insert
    mVenus-p27K- (Cdkn1b Mouse)
    Use
    Lentiviral
    Tags
    mVenus
    Expression
    Mutation
    Mutant K- (lacks affinity to Cdk)
    Promoter
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    176651-plasmid-map-sequence-id-354255

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  12. pWPI

    Plasmid
    #12254
    Purpose
    2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.
    Depositor
    Didier Trono
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    EMCV IRES-EGFP cassette
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12254-plasmid-map-sequence-id-429661

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  13. lenti dCAS-VP64_Blast

    Plasmid
    #61425
    Purpose
    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Lentiviral Prep
    Insert
    dCAS9(D10A, N863A)-VP64_2A_Blast
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    61425-plasmid-map-sequence-id-457486

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  17. pC0043-PspCas13b crRNA backbone

    Plasmid
    #103854
    Purpose
    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6-BbsI-BbsI-PspCas13b DR-polyT
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    103854-plasmid-map-sequence-id-350782

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  18. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid
    #139999
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139999-plasmid-map-sequence-id-346119

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  27. pYPQ203 (pMDC32-Ubi1)

    Plasmid
    #86207
    Purpose
    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker
    Depositor
    Yiping Qi
    Article
    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10
    Type
    Empty backbone
    Use
    Gateway compatible attr1-attr2 destination vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Maize ubiquitin 1 (ZmUbi1)
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86207-plasmid-map-sequence-id-174284

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  33. pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

    Plasmid
    #190163
    Purpose
    Microglia-targeted transgene expression.
    Depositor
    Hirokazu Hirai
    Article
    Okada et al Commun Biol. 2022 Nov 11;5(1):1224. doi:
    Inserts
    mIba1 promoter, 1.7-kb, microglia-specific promoter
    miR-9-Tx4, 4 repeated miRNA target sites for miR-9
    miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    190163-plasmid-map-sequence-id-457470

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  36. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60231
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and hSyn
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60231-plasmid-map-sequence-id-417804

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  38. pLKO.3G

    Plasmid
    #14748
    Depositor
    Diane Mathis, Christophe Benoist
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 5, 2007
    Availability
    Academic Institutions and Nonprofits only
    14748-plasmid-map-sequence-id-352429

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Showing: 241 - 280 of 4124 results