161,488 results
-
-
pcDNA3/Flag-METTL14
Plasmid#53740PurposeFor expression of METTL14 (methyltransferase-like 14) in human cellsDepositorInsertMethyltransferase-like 14 (METTL14 Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRL-mU6-mScarletGO-P2A-Blas-PGK-Neo
Plasmid#136904PurposeLentivirus for base editing activatable mScarletI expression in mammalian cells. All-in-one vector with sgmScarletGO.DepositorInsertmScarletGo-p2a-Blas
UseLentiviralTagsExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-Brn2
Plasmid#27151DepositorInsertBrn2 (Pou3f2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-
Plasmid#115516PurposePlasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporterDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSExpressionMutationPromoterUASAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag
Plasmid#209653PurposeExpression of iATPSnFR2, low affinityDepositorInsertiATPSnFR2.A95A.A119L.HaloTag
UseAAVTagsExpressionMutationA95A.A119LPromoterCAGAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAMKIIa-TetOn3G
Plasmid#133727PurposePlasmid contains the TetOn3G-transactivator sequence under control of a Camk2a promoter.DepositorInsertTetOn3G
UseAAVTagsExpressionMammalianMutationPromoterpCamk2aAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_Donor (EMM0021)
Plasmid#63934Purpose3X Flag with P2A-Neo for 3' tagging of endogenous proteinsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsGlycine-Serine-3XFlag-P2A-NeoRExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
phND2-N174
Plasmid#31822DepositorInsertNeuroD2 (NEUROD2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET22b-msfGFP-NPM1
Plasmid#237670PurposeFor expression of msfGFP-NPM1 in EcoliDepositorInsertNPM1 (NPM1 Human)
UseTagsmsfGFPExpressionBacterialMutationPromoterAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TDP-43 (Q331K)
Plasmid#235488PurposeExpression of human TDP-43-EGFP with human ALS-causing Q331K mutationDepositorInsertTARDBP (TARDBP Human)
UseTagsEGFPExpressionMammalianMutationQ331KPromoterAvailable sinceApril 30, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)
Plasmid#29722DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
6xHsa.NFKB:d2EGFP
Plasmid#239990PurposeDestabilized green fluorescent protein (d2EGFP) under transcriptional control of NF-kB activityDepositorInsertDestabilized EGFP
UseTagsExpressionMutationPromotercfos minimal promoterAvailable sinceJune 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781)
Plasmid#199777Purposeenhancer AAV for Cre recombinase expression in striatal indirect pathway D2-MSNsHas ServiceAAV PHP.eBInsertiCre(R297T)
UseAAVTagsExpressionMutationR297T for reduced recombinase efficiency.PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
SepOTSλ
Plasmid#68292PurposePhosphoserine orthogonal translation system (preferred variant)DepositorInsertSepRS9/EFSep21, 4xtRNA-Sep-A37
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
HRE-oUnaG
Plasmid#201712PurposeHypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1aDepositorInsertUnaG
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFR-mEGFP
Plasmid#203774PurposeNumber and brightness analysis of EGFR oligomerizationDepositorInsertEGFR (EGFR Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-dCas9-3XmCherry
Plasmid#64108PurposeExpression of Sp dCas9-3XmCherry in mammalian cellsDepositorInsertSp dCas9
UseCRISPR and LentiviralTags3XmCherryExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
BFP-Sec61 beta
Plasmid#49154Purposeexpression of Sec61 beta fused to BFP, used as a general ER markerDepositorInsertSec61 beta (SEC61B Human)
UseTagsBFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-EGFP-IRES(Neo)R
Plasmid#158068PurposeeGFP under the TRE3G promoter, Neo resistance. For cloning GOIDepositorInserteGFP
UseTagseGFPExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cyclin E-pBI-EGFP
Plasmid#16654DepositorInsertCyclin E (CCNE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP
Plasmid#21181DepositorInsertenhanced green flourescent protein
UseDrosophila expressionTagsExpressionMutationPromoterAvailable sinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
-
Halo-Cyto
Plasmid#124314PurposeExpresses cytosolic specific HaloTag proteinDepositorInsertHalo tag
UseTagsCytoplasm specific seqExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-IRES eGFP
Plasmid#128059PurposeLentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-Int
Plasmid#50000PurposeExpresses the integrase on a suicide plasmidDepositorInsertIntegrase from pMV361
UseTagsExpressionBacterialMutationPromoterownAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
Plasmid#207863PurposeAAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNADepositorInsertNpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (GFP)
Plasmid#80924PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorTypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP
Plasmid#154139PurposeExpresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.DepositorInsertdCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
UseTags3x FLAG and tagBFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 myc CIITA (P#808)
Plasmid#14650DepositorInsertCIITA (CIITA Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
Plasmid#207860PurposeAAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairsDepositorInsertNpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-UbC-Tet1c-dCas9
Plasmid#232178PurposeLentiviral cassette that expresses Tet1c-dCas9 fusion with a short linker in mammalian cellsDepositorInsertTet1 catalytic domain - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
UseLentiviralTagsFLAGExpressionMammalianMutationD10A, H840APromoterhUbCAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX401-INK4A
Plasmid#121919PurposeDoxycycline-inducible expression of p16-INK4A product of CDKN2ADepositorInsertp16-INK4A (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE Tet ONAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-U6:gRNA
Plasmid#63157PurposeDestination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP-NOP56-C3
Plasmid#134536PurposeExpresses YFP-NOP56 fusion proteinDepositorInsertNOP56 (NOP56 Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
HRE-dUnOHR
Plasmid#201714PurposeHypoxia responsive promoter driving UnaG and mOrangeDepositorInsertUnaG
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB-CMPE-ePPEplus
Plasmid#205246PurposeFor plant prime editing in wheat plantsDepositorInsertCsy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLPCX cyto Grx1-roGFP2
Plasmid#64975PurposeMammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)DepositorInsertGlutaredoxin-1 (GLRX Human)
UseRetroviralTagsroGFP2ExpressionMammalianMutationPromoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GST TEV LIC cloning vector (1G)
Plasmid#29655DepositorTypeEmpty backboneUseTags6xHis, GST, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
CAG-AausFP1-Z1
Plasmid#135047PurposeAausFP1 - bright green fluorescent protein expression from A. australis under CAG promoter in minimal backbone plasmid.DepositorInsertAausFP1
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCAG PromoterAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAS9-mCherry-Frame +2
Plasmid#66941PurposeCRISPaint frame selector +2DepositorInsertgRNA frame +2
UseTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV_Cas9_puro
Plasmid#65655PurposeRetroviral introduction of Cas9 into mammalian cell line.DepositorInsertCas9
UseCRISPR and RetroviralTags5 ' FLAG tagExpressionMammalianMutationPromoterMSSV_LTRAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXG260
Plasmid#141282PurposeCMV:EGFP (as control for pXG237)DepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FGF2-G3
Plasmid#135521PurposeEngineered form of fibroblast growth factor 2 with improved thermostabilityDepositorInsertfibroblast growth factor 2 (FGF2 Human)
UseTags6x His tag and Agg Thrombin TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNP1954
Plasmid#222730PurposepNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)DepositorInsertIS621 bridge RNA
UseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT3GEPIR-CCND1shRNA1
Plasmid#220577PurposeTo inducibly knockdown CCND1 expressionDepositorInsertCCND1 shRNA1 (CCND1 Human)
UseLentiviralTagsExpressionMutationPromoterTRE3G (TetOP) promoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEV53D
Plasmid#44168DepositorInsertEquine infectious anemia virus (EIAV) gag-pol,rev, and S2
UseLentiviralTagsExpressionMammalianMutationA131T mutation in gag; Deletion of viral LTR sequ…PromoterCMVAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3 BRE Luciferase
Plasmid#45126DepositorInsertId1-BRE
UseLuciferase; Bmp/smad transcriptional reporterTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
mito-V5-APEX2
Plasmid#72480PurposeExpresses APEX2 in mitochondriaDepositorInsertAPEX2
UseTagsV5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBT280
Plasmid#122610Purposeexpress evoFERNY-BE4max in mammalian cellsDepositorInsertevoFERNY-BE4max
UseTagsExpressionMammalianMutationH102P D104NPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only
