Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

100+ requests Clear filter

Applied Filters

100+ requests Clear filter

4,203 results

Showing: 281 - 320 of 4203 results

  4. pSpCas9(BB)-2A-Blast

    Plasmid
    #118055
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    118055-plasmid-map-sequence-id-443898

    Enlarge

  7. mEGFP-N1

    Plasmid
    #54767
    Purpose
    Localization: N1 Cloning Vector, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    mEGFP
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54767-plasmid-map-sequence-id-204493

    Enlarge

  10. pET His6 Sumo TEV LIC cloning vector (1S)

    Plasmid
    #29659
    Depositor
    Scott Gradia
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    6xHis, SUMO, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29659-plasmid-map-sequence-id-354447

    Enlarge

  18. pcDNA-GFP-STAT1

    Plasmid
    #11987
    Depositor
    Steven Johnson
    Insert
    Signal transducer and activator of transcription 1 (STAT1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    A188T, Q718R
    Available Since
    July 25, 2007
    Availability
    Academic Institutions and Nonprofits only
    11987-plasmid-map-sequence-id-483719

    Enlarge

  19. pJR85

    Plasmid
    #140095
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140095-plasmid-map-sequence-id-292013

    Enlarge

  29. pFC332

    Plasmid
    #87845
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    hph (hygromycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available Since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87845-plasmid-map-sequence-id-418925

    Enlarge

  31. lentiCRISPR v2-Blast

    Plasmid
    #83480
    Purpose
    Mammalian expression of Cas9 and sgRNA scaffold
    Depositor
    Mohan Babu
    Insert
    Blasticidin S deaminase
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    EF-1α
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83480-plasmid-map-sequence-id-423158

    Enlarge

  33. pdCas9-bacteria

    Plasmid
    #44249
    Purpose
    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 (bacteria)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    pLtetO-1
    Available Since
    April 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    44249-plasmid-map-sequence-id-269588

    Enlarge

  35. pEcCas

    Plasmid
    #73227
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA
    Depositor
    Sheng Yang
    Article
    Li et al Acta Biochim Biophys Sin (Shanghai). 202
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR; Red recombinase
    Expression
    Bacterial
    Promoter
    native promoter
    Available Since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73227-plasmid-map-sequence-id-419024

    Enlarge

Showing: 281 - 320 of 4203 results