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177,146 results

Showing: 3951 - 4000 of 177146 results

  2. CAG-GFP/cre

    Plasmid
    #49054
    Purpose
    Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase
    Depositor
    Fred Gage
    Publication
    Tashiro et al Nat Protoc. 2006;1(6):3049-55.
    Insert
    GFP/Cre
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    49054-plasmid-map-sequence-id-490793

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  9. pDEST51-LRRK2-G2019S

    Plasmid
    #29401
    Depositor
    Mark Cookson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LRRK2 (LRRK2 Human)
    Use
    Gateway Cloning
    Tags
    6XHis and V5
    Expression
    Mammalian
    Mutation
    Gly2019Ser
    Available since
    April 1, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    29401-plasmid-map-sequence-id-256667

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  22. pCMV5-AML1B

    Plasmid
    #12426
    Purpose
    pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.
    Depositor
    Scott Hiebert
    Publication
    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AML1B (RUNX1 Human)
    Expression
    Mammalian
    Available since
    Nov. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    12426-plasmid-map-sequence-id-151854

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  26. pTiger

    Plasmid
    #1728
    Purpose
    This is a 3rd generation lentiviral transfer vector (highest titer) from Nolan lab's FELIX vector system
    Depositor
    Garry Nolan
    Type
    Empty backbone
    Use
    Lentiviral
    Available since
    Aug. 18, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    1728-plasmid-map-sequence-id-105963

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  31. pET-pA-Hia5

    Plasmid
    #174372
    Purpose
    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Publication
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein A - hia5
    Tags
    6xHis and Protein A
    Expression
    Bacterial
    Available since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    174372-plasmid-map-sequence-id-341860

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  32. pLX_EFS-Cre_ppt-del

    Plasmid
    #162073
    Purpose
    Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)
    Depositor
    Robert Manguso
    Publication
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Insert
    Cre
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EFS (part of insert)
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    162073-plasmid-map-sequence-id-325292

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  37. pmCherry-2xFYVE

    Plasmid
    #140050
    Purpose
    Expresses mCherry-tagged HRS 2xFYVE in mammalian cells
    Depositor
    Harald Stenmark
    Publication
    Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tandem HRS FYVE domain (Hgs Mouse)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    two FYVE domains (amino acids 147-223), linked by…
    Promoter
    CMV
    Available since
    May 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    140050-plasmid-map-sequence-id-296063

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  38. Flag-APEX2-C Cloning Vector

    Plasmid
    #128146
    Purpose
    To express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEM
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Tags
    Flag-APEX2
    Expression
    Mammalian
    Available since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128146-plasmid-map-sequence-id-248610

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  40. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    107942-plasmid-map-sequence-id-259760

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  41. pUCM-CLYBL-hNIL

    Plasmid
    #105841
    Purpose
    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron
    Depositor
    Michael Ward
    Publication
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    105841-plasmid-map-sequence-id-490369

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Showing: 3951 - 4000 of 177146 results