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159,238 results

Showing: 3951 - 4000 of 159238 results

  3. pCAG-N2cL

    Plasmid
    #100812
    Purpose
    express rabies virus CVS N2c strain polymerase gene (N2cL)
    Depositor
    Ian Wickersham
    Insert
    rabies virus CVS N2c strain polymerase gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    100812-plasmid-map-sequence-id-189505

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  4. pCMV-PE6b

    Plasmid
    #207852
    Purpose
    Mammalian expression of PE6b prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6b
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207852-plasmid-map-sequence-id-408170

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  5. pCoofy1-psHaloTag1a

    Plasmid
    #232940
    Purpose
    Photoswichable HaloTag: psHaloTag1a variant
    Depositor
    Claire Deo
    Article
    Walterspiel et al bioRxiv 2024.12.18.629107
    Insert
    photoswitchable HaloTag 1a variant
    Use
    Tags
    His6x-HRV3C
    Expression
    Bacterial
    Mutation
    E143W, A258W with linkers L1 (FAG) and L2 (P)
    Promoter
    T7
    Available since
    March 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    232940-plasmid-map-sequence-id-464327

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  6. pCAG-N2cN

    Plasmid
    #100801
    Purpose
    express rabies virus CVS N2c strain nucleoprotein gene (N2cN)
    Depositor
    Ian Wickersham
    Insert
    rabies virus CVS N2c strain nucleoprotein gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    100801-plasmid-map-sequence-id-189511

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  7. pCAG-N2cP

    Plasmid
    #100808
    Purpose
    express rabies virus CVS N2c strain phosphoprotein gene (N2cP)
    Depositor
    Ian Wickersham
    Insert
    rabies virus CVS N2c strain phosphoprotein gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    100808-plasmid-map-sequence-id-189510

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  8. pBiFC-PBZ-4A-VC

    Plasmid
    #110649
    Purpose
    expresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR binding
    Depositor
    Chris Lord
    Article
    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:
    Insert
    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    Use
    Tags
    HA and VC
    Expression
    Mammalian
    Mutation
    C379A, C385A, C421A and C427A
    Promoter
    CMV
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110649-plasmid-map-sequence-id-217742

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  10. pCDZ2FE_Pogz_1-1410

    Plasmid
    #105674
    Purpose
    Expresses Flag-tagged full-length human POGZ protein
    Depositor
    Thanos Halazonetis
    Insert
    POGZ (POGZ Human)
    Use
    Tags
    2X Flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105674-plasmid-map-sequence-id-203198

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  14. hC73S-pEGFP-N1

    Plasmid
    #111536
    Purpose
    Expresses human KCNB1 C73S in mammalian cells for imaging
    Depositor
    Federico Sesti
    Insert
    human C73S-Kv2.1 (KCNB1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    C73S
    Promoter
    CMV
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111536-plasmid-map-sequence-id-211633

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  18. pAAV-hSyn-DIO {hCAR}off-{GCaMP6f}on-W3SL

    Plasmid
    #111394
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON GCaMP6f (ultrasensitive calcium sensor), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    hCAR (CXADR Human)
    GCaMP6f
    Use
    AAV
    Tags
    6xHis, Myc, T7, and Xpress
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    111394-plasmid-map-sequence-id-212394

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  28. pTarget

    Plasmid
    #214052
    Purpose
    Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Target sequence
    Use
    Target rna plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214052-plasmid-map-sequence-id-428822

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  29. pNon-target

    Plasmid
    #214053
    Purpose
    Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Non-target sequence
    Use
    Non-target rna plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214053-plasmid-map-sequence-id-428839

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  30. pHochTypeIII

    Plasmid
    #214039
    Purpose
    Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Cas10
    Use
    CRISPR
    Tags
    Expression
    Mutation
    WT
    Promoter
    T7 promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214039-plasmid-map-sequence-id-424943

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  37. TFORF0208

    Plasmid
    #142556
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXP2 (FOXP2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    142556-plasmid-map-sequence-id-427312

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  39. pSBbi_RP_2XMTS-MicroID2-FLAG

    Plasmid
    #188524
    Purpose
    Encodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cells
    Depositor
    James Londino
    Article
    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.
    Insert
    2XMTS-MicroID2
    Use
    Transposon mediated gene integration
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188524-plasmid-map-sequence-id-372835

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  42. pTripz NEO DEST

    Plasmid
    #209072
    Purpose
    Modified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interest
    Depositor
    Matthew Oser
    Article
    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector to be used…
    Tags
    3X FLAG
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Nov. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    209072-plasmid-map-sequence-id-412301

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  43. pHis17_ParM _1

    Plasmid
    #78201
    Purpose
    Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor
    Depositor
    Martin Webb
    Article
    Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8
    Insert
    parM (K33A I27C T174A T175N C287A)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    K33A I27C T174A T175N C287A
    Promoter
    unknown
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    78201-plasmid-map-sequence-id-151697

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  46. TFORF1906

    Plasmid
    #141883
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCF7L2 (TCF7L2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141883-plasmid-map-sequence-id-415059

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  49. ND4.2-Left TALE-G1397-N-DddA11-mCherry

    Plasmid
    #179682
    Purpose
    Expression of base editor in mammalian cells and for cell sorting
    Depositor
    David Liu
    Article
    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387
    Insert
    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_P2A_mCherry_ATP5B 3'UTR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S1330I A1341V N1342S E1370K T1380I
    Promoter
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    179682-plasmid-map-sequence-id-355098

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Showing: 3951 - 4000 of 159238 results