163,517 results

1. drebrin-YFP

   Plasmid

   #40359

   Depositor

   Phillip Gordon-Weeks

   Article

   Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

   Insert

   drebrin E2 (DBN1 Human)
   

   Use

   Chick expression

   Tags

   eYFP

   Expression

   Mutation

   Promoter

   chick B-actin promoter with CMV enhancer

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. K44A dynamin 1 pEGFP

   Plasmid

   #34681

   Depositor

   Sandra Schmid

   Article

   Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

   Insert

   dynamin 1ba (DNM1L Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   Available Since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC13N-dCas9-BFP-KRAB

   Plasmid

   #127968

   Purpose

   constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   CLYBL-CAG-dCas9-NLS-BFP-KRAB
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmTurquoise2-T2A-Venus(L68V)

   Plasmid

   #60494

   Purpose

   Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   mTurquoise2-T2A-mVenus(L68V)
   

   Use

   Tags

   T2A peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRluc-LC3G120A

   Plasmid

   #105003

   Purpose

   Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

   Depositor

   Marja Jããttelã

   Article

   Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Luciferase

   Tags

   Rluc with C124A substitution

   Expression

   Mammalian

   Mutation

   Glycine 120 mutated to alanine, prevents lipidati…

   Promoter

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmGFP-P2A-K0-P2A-RFP

   Plasmid

   #105686

   Purpose

   Double fluorescent stalling reporter K0

   Depositor

   Ramanujan Hegde

   Article

   Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

   Inserts

   P2A-3xFLAG-VHPbeta
   K0
   P2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (from vector)

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1 Puro shRNA Scramble

   Plasmid

   #162011

   Purpose

   Lentiviral negative control vector containing scramble shRNA

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   scramble shRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMH-SFB-DYRK1A

   Plasmid

   #101770

   Purpose

   Mammalian expression of DYRK1A fusion protein

   Depositor

   Michael Huen

   Article

   Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

   Insert

   DYRK1A (DYRK1A Human)
   

   Use

   Tags

   S peptide-flag-streptavidin binding peptide (SFB)

   Expression

   Mammalian

   Mutation

   siRNA-resistant allele

   Promoter

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mcherry-PH

   Plasmid

   #36075

   Depositor

   Narasimhan Gautam

   Article

   Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

   Insert

   PH domain of PLCdelta1 (PLCD1 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. LifeAct-mGarnet2

    Plasmid

    #104308

    Purpose

    codes for a fusion of fluorescent protein mGarnet2 and lifeact that targets F-actin in live cells

    Depositor

    Ulrich Nienhaus

    Article

    Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

    Insert

    LifeAct-mGarnet2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Parkin

    Plasmid

    #187897

    Purpose

    Mammalian cell expression of untagged Parkin

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    PRKN (PRKN Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRluc-LC3wt

    Plasmid

    #105002

    Purpose

    Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

    Depositor

    Marja Jããttelã

    Article

    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Luciferase

    Tags

    Rluc with C124A substitution

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE-ERBB3

    Plasmid

    #116735

    Purpose

    Lentiviral expression of ERBB3

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB3 (ERBB3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Mito-B

    Plasmid

    #209864

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    Targeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_AncBE4max

    Plasmid

    #112094

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    AncBE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSSM110: pCMV-HA-NLS-SpuFz1-v2

    Plasmid

    #205277

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    C310R/D487K/T513K

    Promoter

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMVTNT PINK1 C-myc

    Plasmid

    #13314

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15b-AavLEA1

    Plasmid

    #53093

    Purpose

    AavLEA1 expression (in BL21 DE3 E. coli strain)

    Depositor

    Claude Férec

    Article

    Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

    Insert

    AavLEA1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. ER-GA

    Plasmid

    #209871

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Use

    Tags

    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1 - TRC mTagBFP2

    Plasmid

    #191566

    Purpose

    Expresses an shRNA and mTagBFP2

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PDG458 V3

    Plasmid

    #226959

    Purpose

    CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmiHiFiCas9

    Plasmid

    #164832

    Purpose

    miHiFiCas9 expressing plasmid

    Depositor

    Eugene Chen

    Article

    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

    Insert

    miHiFiCas9 (contains Brex27 at sequence 5031-5138).
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mammalian

    Mutation

    change R 691 to A

    Promoter

    CMV

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHR-EF1Alpha-Tet-on 3G

    Plasmid

    #118592

    Purpose

    Expresses Tet-on 3G from the EF1Alpha promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Tet-on 3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-dCas13-M3M14nes

    Plasmid

    #155367

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Promoter

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMU-TPr

    Plasmid

    #61189

    Purpose

    Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    AtTIP1-1
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. sfTq2ox-PBP5

    Plasmid

    #117960

    Purpose

    Periplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    sfTq2ox-PBP5 (dacA )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EGFP-Actin-7

    Plasmid

    #56421

    Purpose

    Localization: Actin, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. JNK-KTR-mStrawberry reporter

    Plasmid

    #158689

    Purpose

    This plasmid is a JNK pathway reporter. It has CMV promoter driving the expression of JNK-KTR fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. paavCAG-pre-mGRASP-mCerulean

    Plasmid

    #34910

    Purpose

    pre

    Depositor

    Jinhyun Kim

    Article

    Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

    Has Service

    AAV5

    Insert

    presynaptic mGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. V405 4HA-CKIe

    Plasmid

    #13724

    Depositor

    David Virshup

    Article

    Rivers et al J Biol Chem. 1998 Jun 26. 273(26):15980-

    Insert

    casein kinase 1 epsilon (CSNK1E Human)
    

    Use

    Tags

    4 HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDH-EF1-Luc2-P2A-copGFP

    Plasmid

    #72485

    Purpose

    Express Luc2 and copGFP

    Depositor

    Kazuhiro Oka

    Insert

    Luc2-P2A-copGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1

    Available Since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pCMV_ABEmax_P2A_GFP

    Plasmid

    #112101

    Purpose

    A:T-to-G:C base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    ABEmax_P2A_GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-CaMKII(0.3)-mVenus-FKBP-K7GEF

    Plasmid

    #225828

    Purpose

    A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

    Depositor

    Haruo Kasai

    Article

    Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

    Insert

    mVenus-FKBP-K7GEF
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEF1α-Cre-2A-Puro

    Plasmid

    #207743

    Purpose

    Expression of the Cre recombinase linked to a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-2A-Puro
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pLX - T7pro - EBFP

    Plasmid

    #138615

    Purpose

    Express EBFP with an upstream T7 promoter in a lentiviral backbone

    Depositor

    Fei Chen

    Article

    Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    EBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GFP-EEA1 wt

    Plasmid

    #42307

    Depositor

    Silvia Corvera

    Article

    Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHR-mCh-Cry2olig

    Plasmid

    #101222

    Purpose

    Cry2olig(E490G)

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2olig
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    SFFV

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAV-syn-ChR2-EYFP-Kv

    Plasmid

    #89256

    Purpose

    Channelrhodopsin localized to neuronal soma via a Kv2.1 targeting motif

    Depositor

    McLean Bolton

    Article

    Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

    Insert

    Channelrhodopsin
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    synapsin

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LentiGuide-puro-NLS-mCherry

    Plasmid

    #185474

    Purpose

    Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. LXRE_Luc

    Plasmid

    #177622

    Purpose

    synthetic 3X LXRE promoter linked to luciferase in the pTAL vector

    Depositor

    Thomas Burris

    Article

    Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

    Insert

    3X LXRE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEGFP-C1-ADRP

    Plasmid

    #87161

    Purpose

    Fluorescent protein localized to lipid droplets (LDs)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ADRP (PLIN2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. TfR-pHuji

    Plasmid

    #61505

    Purpose

    Human transferrin receptor tagged with pH sensitive red fluorescent protein pHuji

    Depositor

    David Perrais

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    Transferrin receptor (TFRC Human)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pMA4008

    Plasmid

    #70109

    Purpose

    mtDNA destruction/production of rho-0 cells

    Depositor

    Mikhail Alexeyev

    Article

    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

    Inserts

    EGFP
    Herpes simplex virus UL12.5M185
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    N-terminal deletion of UL12.5

    Promoter

    CMV and RSV

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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45. pcDNA3.2/v5-DEST hGlut1

    Plasmid

    #18085

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    Glut1 (SLC2A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid

    #107942

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    

    Use

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R, and N282A

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pMo130

    Plasmid

    #27388

    Purpose

    Burkholderia allelic exchange suicide vector pMo130, ref EU862243

    Depositor

    Martin Voskuil

    Article

    Hamad et al Gene. 2009 Feb 1. 430(1-2):123-31.

    Type

    Empty backbone

    Use

    Burkholderia allelic exchange suicide vector pmo1…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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48. gRNA_GFP-T1

    Plasmid

    #41819

    Purpose

    Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_GFP-T1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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49. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid

    #162277

    Purpose

    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.

    Depositor

    Kazuhisa Nakayama

    Article

    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi

    Insert

    HiFi Cas9
    

    Use

    CRISPR

    Tags

    BPNLS and FLAG tag

    Expression

    Mammalian

    Mutation

    SpCas9 (R691A)

    Promoter

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAV hSyn FLEx-FRT hM4Di-mCherry

    Plasmid

    #161576

    Purpose

    Expression of hM4Di-mCherry in a Flp-dependent fashion

    Depositor

    Robert Malenka, Kevin Beier

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    hM4Di-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    hSyn

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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