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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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8,630 results

Showing: 4001 - 4050 of 8630 results

  1. HR_G3BP1-V5-APEX2-GFP

    Plasmid
    #105284
    Purpose
    homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus
    Depositor
    Eugene Yeo
    Article
    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do
    Insert
    G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)
    Use
    Human homologous recombination donor
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105284-plasmid-map-sequence-id-229022

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  3. pcDNA3.1-ccdB-Nanoluc

    Plasmid
    #87067
    Purpose
    Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    87067-plasmid-map-sequence-id-192710

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  6. TAp63alpha-FLAG

    Plasmid
    #27008
    Depositor
    David Sidransky
    Insert
    TAp63alpha (TP63 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 15, 2010
    Availability
    Academic Institutions and Nonprofits only
    27008-plasmid-map-sequence-id-169367

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  11. DRD2-Tango

    Plasmid
    #66269
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    DRD2 (DRD2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66269-plasmid-map-sequence-id-157899

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  16. Lamp1-YFP

    Plasmid
    #1816
    Purpose
    Mammalian expression of Lamp1 fused to YFP. Used for localization to the lysosome.
    Depositor
    Walther Mothes
    Article
    Sherer et al Traffic 2003 Nov;4(11):785-801.
    Insert
    lysosome associated membrane protein 1 (Lamp1 Rat)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    D50E (not important for function of plasmid)
    Promoter
    Available since
    Feb. 16, 2006
    Availability
    Academic Institutions and Nonprofits only
    1816-plasmid-map-sequence-id-364671

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  26. Flag-APEX2-C Cloning Vector

    Plasmid
    #128146
    Purpose
    To express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEM
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Flag-APEX2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128146-plasmid-map-sequence-id-248610

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  27. pSB700

    Plasmid
    #64046
    Purpose
    Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein
    Depositor
    George Church
    Article
    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, CAGGS
    Available since
    May 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64046-plasmid-map-sequence-id-313670

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  30. pcDNA3-TLR1-YFP

    Plasmid
    #13014
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 1 (TLR1 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13014-plasmid-map-sequence-id-237573

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  42. R4pGWB601_UBQ10p-Turbo-YFP-NLS

    Plasmid
    #127368
    Purpose
    Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Plant
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127368-plasmid-map-sequence-id-246345

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  45. pcDNA3-Clover

    Plasmid
    #40259
    Purpose
    GFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Article
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has Service
    Cloning Grade DNA
    Insert
    Clover GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE
    Available since
    Sept. 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    40259-plasmid-map-sequence-id-215680

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  50. RedTrackCMV

    Plasmid
    #50957
    Purpose
    A backbone for adenovirus creation by the AdEasy method, similar to AdTrackCMV (Addgene Plasmid # 16405), except it co-expressed mRFP instead of eGFP
    Depositor
    James Bamburg
    Type
    Empty backbone
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    50957-plasmid-map-sequence-id-76710

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Showing: 4001 - 4050 of 8630 results