We narrowed to 31,648 results for: REP
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Plasmid#139196Purposenon-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puroDepositorInsertCBRed
UseLuciferase and RetroviralTagsd2 PEST domain and nuclear localization signal (N…ExpressionMammalianAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
ER-ABKAR
Plasmid#61508PurposeExpression of AMPK biosensor targeted to the endoplasmic reticulumDepositorInsertAMPK Biosensor
TagsER targeting sequence from CYP450 2C1 (see commen…ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAM-DCA-muGFP
Plasmid#166727PurposeAAV, encodes monomeric ultrastable GFP (muGFP)DepositorInsertmuGFP
UseAAVPromoterChicken Beta ActinAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH
Plasmid#74289PurposepAAV vector to express H2B-GFP and oG (optimized Glycoprotein) in a Cre-dependent mannerDepositorInsertH2B-GFP and oG (optimized Glycoprotein)
UseAAVExpressionMammalianMutationchimeric glycoproteinPromoterEf1aAvailable SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 WT1 +1 ATG
Plasmid#217643PurposeDox inducible WT1 cDNA - as transcribed from +1ATG (long isoform)DepositorInsertWT1 long isoform (WT1 Human)
ExpressionMammalianAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-Eevee-ROCK-NES
Plasmid#209918PurposeA FRET biosensor for ROCK activityDepositorInsertEevee-ROCK-NES
UseLentiviralTagsECFP and YPetAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(0)-T2A-Gal4
Plasmid#62899PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0DepositorInsertT2A-Gal4
Available SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
LEPZ
Plasmid#111161PurposemiR-E (miR-30 variant)-based RNAi with ZsGreen reporterDepositorInsertmiR-E (miR-30 variant)
UseRetroviralMutationWTAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)
Plasmid#62956PurposeSinglet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4-Hsp70
Available SinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT-GEM(0)
Plasmid#62891PurposeTrojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneAvailable SinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
PM-ABKAR
Plasmid#61506PurposeExpression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequenceDepositorInsertAMPK Biosensor
TagsLyn kinase sequence (see comments)ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXJ-Myc-KLF2
Plasmid#232654Purposetransient expresses KLF2 in mammalian cellsDepositorAvailable SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G BCL2L11 Neo
Plasmid#140747PurposeInducibly expresses BIM in mammalian cellsDepositorAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-DARPinOFF7
Plasmid#182398PurposeBacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusionDepositorInsertVNp6-DARPinOFF7
TagsVesicle Nucleating peptide 6 (VNp6)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-MLC2 WT
Plasmid#195235PurposeMammalian expression of GFP tagged MLC2. Wild-typeDepositorInsertMLC2 (MYL2 Human)
ExpressionMammalianAvailable SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-YAP1
Plasmid#79503PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDSDepositorAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB
Plasmid#216233PurposeS. aureus USA300 GyrB Codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialPromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK283 (Stag-3FLAG-Neo)
Plasmid#72799PurposeC-terminal tagging with Stag-3FLAG using CRIPSR/Cas9DepositorInsertStag-3FLAG-Neo
UseCRISPRTagsStag-3FLAGExpressionMammalianAvailable SinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_HMOX1-V5-APEX2
Plasmid#85823PurposeExpressed C-term tagged APEX2 on HMOX1 in mammalian cellDepositorInsertHMOX1-V5-APEX2 (HMOX1 Human, Synthetic)
TagsAPEX2 and V5ExpressionMammalianPromoterCMV-FAvailable SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 (Delta Pest1)
Plasmid#78096Purposemammalian expression of GADD34 Delta Pest1DepositorInsertGADD34 (PPP1R15A Human)
TagsFLAGExpressionMammalianMutationdeletion of PEST1PromoterCMVAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee-Kras
Plasmid#140838Purposemammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertSpiCee-Kras
TagsmRFPExpressionMammalianPromoterCAGAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGK EGFP-PPM1H-WT
Plasmid#200084PurposeMammalian expression of wild-type PPM1HDepositorAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE
Plasmid#89587PurposeFor nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.DepositorInsertnuclear localization signal (nls) turboRFP
ExpressionMammalianAvailable SinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABR IRFP-Bcd-LEXY
Plasmid#182595PurposeDrosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorAvailable SinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EBFP2-P2A-EGFP
Plasmid#184938PurposeExpresses blue fluorescent protein EBFP2 and EGFP in mammalian cellsDepositorInsertEBFP2-P2A-EGFP
UseAAVExpressionMammalianPromoterCAGAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF (VE5596)
Plasmid#139768PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette.DepositorTypeEmpty backbonePromoterPH or p10Available SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-eGFP-P2A-LgBiT-NLS
Plasmid#187834PurposeExpression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.DepositorInserteGFP-P2A-LgBiT-NLS
UseAAVPromoterhSynAvailable SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-NIrD
Plasmid#65010PurposeNS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infectionDepositorInsertsrtTA-MAVS(C)
2A-linked delta-TK
UseLentiviralTagsmCherryExpressionMammalianPromoterTight-TREAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPaxillin-Electra1-N1
Plasmid#179484PurposeExpresses paxillin fused to blue fluorescent protein Electra1DepositorInsertpaxillin-Electra1
TagsElectra1ExpressionMammalianPromoterCMVAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)
Plasmid#62954PurposeSinglet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4-Hsp70
Available SinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_VNp-mNeongreen_CydAB-mCherry
Plasmid#182391PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusionsDepositorInsertsVNp-mNeongreen-His
cydAB-mCherry
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-hNKD2
Plasmid#174328Purposeencodes human NKD2 cDNA with a C-terminal GFP-fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
TagsGFP tagExpressionMammalianPromoterCMVAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)
Plasmid#62955PurposeSinglet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4-Hsp70
Available SinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A1.2-c002
Plasmid#173088PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A1 (MACROH2A1 Human)
TagsmCeruleanExpressionBacterialMutationQ162-N372, codon optimizedAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔKinase
Plasmid#163404PurposeExpresses BMPR-2 lacking the kinase domain in mammalian cellsDepositorAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
2xCBX7CD_W35A-IFP2.0C
Plasmid#215755PurposeExpresses binding-deficient W35A mutant of 2xCBX7 chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K27me3 detection in BiAD sensorsDepositorInsert2xCBX7CD_W35A-IFP2.0C
ExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_mup53_Ex5
Plasmid#85535PurposeInducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporterDepositorInsertmu p53
UseCRISPR and LentiviralExpressionMammalianPromoterH1tAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ-mNeongreen
Plasmid#182392PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusionDepositorInsertVNp-LZ-mNeongreen
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJP-HBB-nLuc
Plasmid#175431PurposeExpresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.DepositorInsertnLUC plus 5' HBB leader
ExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPV-H1-ccdB-mEF1α-RiH
Plasmid#100598PurposepiggyBac vector for sgRNA cloningDepositorTypeEmpty backboneUsePiggybac vectorExpressionMammalianPromoterHuman H1 PolIII promoter, human FTH1 promoter, mo…Available SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG
Plasmid#174892Purposeamber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassetteDepositorInsertsfGFP
ExpressionMammalianMutation150TAG in sfGFPPromoterEF1Available SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA
Plasmid#159283PurposeA multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).DepositorInsertsHuman INS sgRNA
Human INS guide RNA
ExpressionMammalianPromoterU6Available SinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-VEGFprom(-500 to -1)
Plasmid#66130PurposeLuciferase-based reporter for VEGF promoter region (-500 to -1 bp)DepositorInsertVascular Endothelial Growth Factor-A promoter region (VEGFA Human)
UseLuciferasePromoterVEGF -500 to -1Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PXPR007 sgGFP-1
Plasmid#74960PurposeCas9 + sgGFP-1 with blasticidin selectionDepositorInsertGFP
UseCRISPRExpressionMammalianAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3
Plasmid#62957PurposeTriplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4 in three phases
Available SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(1)-T2A-Gal4
Plasmid#62900PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1DepositorInsertT2A-Gal4
Available SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPR
Plasmid#199233PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProepiregulin preproprotein (EREG Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-OVOL1-T2A-PuroR
Plasmid#162347PurposeLentiviral expression of OVOL1 under the control of the TetON promoterDepositorAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-eCas9-sgMIB1
Plasmid#140239PurposeLentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1DepositorInsertsgRNA targeting human MIB1
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2Δ751-813aa
Plasmid#163603PurposeExpresses BMPR-2 lacking the LIMK-binding region in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
ExpressionMammalianMutationThe LIMK-binding region (751-813 aa) is deletedAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only