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Showing: 4101 - 4150 of 157662 results

  1. pAK005

    Plasmid
    #233642
    Purpose
    For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.
    Depositor
    Zak Swartz
    Article
    Clarke et al Development. 2024 Oct 15;151(20):dev2028
    Insert
    eSpCas9(1.1) with c-terminal VitelloTagB
    Use
    Tags
    6-His
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    233642-plasmid-map-sequence-id-469019

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  2. pYPQAT57

    Plasmid
    #223429
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223429-plasmid-map-sequence-id-453502

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  3. pYPQAT59

    Plasmid
    #223431
    Purpose
    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223431-plasmid-map-sequence-id-453576

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  4. pYPQAT03

    Plasmid
    #223375
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223375-plasmid-map-sequence-id-452219

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  5. pYPQAT05

    Plasmid
    #223377
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223377-plasmid-map-sequence-id-454173

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  17. pcDNA3.1-Zeo_BACE1

    Plasmid
    #227105
    Purpose
    BACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assays
    Depositor
    Michael Wehr
    Insert
    BACE1 (BACE1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227105-plasmid-map-sequence-id-454377

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  18. pcDNA5-FRT-TOgw-C-mCherry

    Plasmid
    #223952
    Purpose
    Gateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223952-plasmid-map-sequence-id-450834

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  24. pUt-mPF-GFP

    Plasmid
    #199222
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    rtTA-Advanced::P2A::EGFP
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    tight TRE promoter
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199222-plasmid-map-sequence-id-396540

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  26. pLenti-0.5Syn-rtTA-WPRE-pA

    Plasmid
    #183707
    Purpose
    Lenti vector designed to express rtTA from a 0.5 Synapsin promoter
    Depositor
    Jonathan Ploski
    Insert
    rtTA
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183707-plasmid-map-sequence-id-360836

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  32. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

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  41. eMscL G22S

    Plasmid
    #107455
    Purpose
    eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Francesco Difato
    Article
    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Mutation
    Bearing a glycine to serine substitution at posit…
    Promoter
    human synapsin 1
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    107455-plasmid-map-sequence-id-207549

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  42. CoV2-Spike-D614G

    Plasmid
    #177960
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177960-plasmid-map-sequence-id-348179

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  43. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid
    #196716
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-KRAB(ZIM3)
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196716-plasmid-map-sequence-id-388040

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  46. TFORF0239

    Plasmid
    #142385
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MYCL (MYCL Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142385-plasmid-map-sequence-id-391445

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Showing: 4101 - 4150 of 157662 results