159,557 results

1. pTP396

   Plasmid

   #149336

   Purpose

   Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

   Depositor

   Rebecca Voorhees

   Article

   Pleiner et al Science. 2020 May 21. pii: science.abb50

   Insert

   anti-GFP nanobody
   

   Use

   Tags

   Biotin acceptor peptide (Avi-tag), His14, and SUM…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. RFP Dynamin2 K44A

   Plasmid

   #128153

   Purpose

   Expression of Dynamin 2 K44A

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chua et al Proc Natl Acad Sci U S A. 2009 Nov 30. (

   Insert

   Dynamin 2 (Dnm2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBAD-I-SceI

   Plasmid

   #60960

   Purpose

   Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

   Depositor

   Nikolaus Osterrieder

   Article

   Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

   Insert

   I-SceI restriction enzyme
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX330-AAVS1

   Plasmid

   #227272

   Purpose

   Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting AAVS1 (AAVS1 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTRIP-SFFV-mtagBFP-2A STING

   Plasmid

   #102586

   Purpose

   Lentivector to express STING and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHBS1147 GFP-TDP43

   Plasmid

   #118797

   Purpose

   To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDA303

   Plasmid

   #195199

   Purpose

   Overexpressing 6His-TetR in E. coli.

   Depositor

   Rahul Sarpeshkar

   Article

   Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

   Insert

   TetR
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCap-101-Hgy

   Plasmid

   #227508

   Purpose

   Capture plasmid with hygromycin resistance and pIJ101 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

   Plasmid

   #105648

   Purpose

   Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks

   Depositor

   Sandra Rieger

   Insert

   Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET30-HPF1-His-Sumo-Flag

    Plasmid

    #111577

    Purpose

    Expresses full length HPF1 in E. coli

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    HPF1 (HPF1 Human)
    

    Use

    Tags

    6xHis-Sumo-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-Myc-NLRP3(R260W)

    Plasmid

    #73956

    Purpose

    Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

    Insert

    NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    R260W

    Promoter

    CMV iE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pscALPS- SIV-MAC251 vpx

    Plasmid

    #115810

    Purpose

    Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251 Vpx
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AiP13098 - pAAV-AiE0380h-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN3098)

    Plasmid

    #208123

    Purpose

    Direct-expressing SYFP2 in astrocytes AAV Virus

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Mich et al bioRxiv 2023.09.20.558718

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAV-CAG-oScarlet-WPRE-BC1-pA

    Plasmid

    #220765

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al bioRxiv 2024.04.24.590975

    Insert

    oScarlert-WPRE-BC1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAV-CAG-oScarlet-WPRE-BC2-pA

    Plasmid

    #220766

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al bioRxiv 2024.04.24.590975

    Insert

    oScarlert-WPRE-BC2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-C1(2)delta

    Plasmid

    #21216

    Depositor

    Tobias Meyer

    Article

    Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

    Insert

    GFP-N2-PKCdelta-C1 (Prkcd Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C1(2) domains of PKC delta (rat) (Lab plasmid WC…

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYZ463

    Plasmid

    #187971

    Purpose

    TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a - TR

    Plasmid

    #89621

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Thioredoxin
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ER-GCaMP6-210

    Plasmid

    #86919

    Purpose

    Fluorescent reporter for ER calcium signaling

    Depositor

    Timothy Ryan

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    ER-GCaMP6-210
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

    Promoter

    CAMKII

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRel-Hyg

    Plasmid

    #227582

    Purpose

    Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPBT/EFS-Cas9-PuroTk

    Plasmid

    #170542

    Purpose

    Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    PuroTK
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-VEGFR1-V5/HIS

    Plasmid

    #205616

    Purpose

    Expression of human VEGFR1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    VEGFR1 receptor tyrosine kinase (FLT1 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRSETB/mStayGold(c4)

    Plasmid

    #212018

    Purpose

    For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    mStayGold(c4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSFDuet-1_VNp-DNAseI

    Plasmid

    #182409

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-DNase I (DNASE1 Bovine)
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLminP_Luc2P_RE6

    Plasmid

    #90340

    Purpose

    STAT5 - Stat5 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    STAT5

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDONR223_KRAS_p.G12S

    Plasmid

    #81654

    Purpose

    Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    G12S

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR223_KRAS_p.G12R

    Plasmid

    #82804

    Purpose

    Gateway Donor vector containing KRAS , part of the Target Accelerator Plasmid Collection.

    Depositor

    Matthew Meyerson, David Root, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    G12R

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLD-puro-Cc-TARDBP-WT_VA

    Plasmid

    #141327

    Purpose

    Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-WT in mammalian cells

    Depositor

    Mohan Babu

    Article

    Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

    Insert

    TARDBP-WT (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRM055 His-TEV-Cas9 6xNLS 0C

    Plasmid

    #196246

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p2K7-bsd-UBI-tagRFP-KDEL

    Plasmid

    #114179

    Purpose

    Lentiviral vector for expression of tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    tagRFP-KDEL
    

    Use

    Lentiviral

    Tags

    KDEL retention signal and signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCF153_Gag-Pol

    Plasmid

    #225961

    Purpose

    CMV-Intron-GagPol (FMLV). Expresses FMLV (Friend Murine Leukemia Virus) Gag-Pol for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-GagPol (FMLV)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEGFP PICH (Nigg CB62)

    Plasmid

    #41163

    Depositor

    Erich Nigg

    Article

    Baumann et al Cell. 2007 Jan 12;128(1):101-14.

    Insert

    PICH (ERCC6L Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid

    #119946

    Purpose

    Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetCMV

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFREE_Zeo

    Plasmid

    #92053

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CTGF-bio-His

    Plasmid

    #52028

    Purpose

    Expresses full-length Connective tissue growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CTGF (CCN2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFp10

    Plasmid

    #170460

    Purpose

    EarlyBac insect cell expression system

    Depositor

    Hiro Furukawa

    Article

    Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

    Insert

    none
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dHsp70

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDEST47-SAR1-GFP

    Plasmid

    #67409

    Purpose

    Mammalian expression of hSar1 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SAR1 (SAR1A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

38. GAG-PEmax V7

    Plasmid

    #232921

    Purpose

    Optimized construct for the expression of PEmax fused to GAG from fMLV. This plasmid is involved in the production of NanoScribes

    Depositor

    Philippe Mangeot

    Article

    Halegua et al Nat Commun. 2025 Jan 4;16(1):397. doi: 1

    Insert

    GAG FMLV fused to PEmax
    

    Use

    CRISPR

    Tags

    Nuclear export signals (x3)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. lenti-sgRNA puro

    Plasmid

    #104990

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-FLAG-FADD

    Plasmid

    #78802

    Purpose

    To overexpress FADD in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Nat Commun. 2012;3:978. doi: 10.1038/nco

    Insert

    FADD (FADD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. UPB-stomach (UPB1)

    Plasmid

    #180784

    Purpose

    Unique Projection Barcode (UPB1)-Stomach for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1 TEV (full-length)

    Plasmid

    #64276

    Purpose

    Expresses tobacco etch virus protease (TEV)

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Tobacco etch virus (TEV) protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pcDNA3.1 LIC 6A CARD8-FLAG

    Plasmid

    #169948

    Purpose

    Mammalian expression of CARD8-FLAG

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. PB-CT3G-CERP2-MG

    Plasmid

    #175503

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pDONR221_SLC1A4

    Plasmid

    #131962

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC1A4 (SLC1A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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46. pME-mTagBFP

    Plasmid

    #75148

    Purpose

    TagBFP (blue) fluorophore for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mTagBFP
    

    Use

    Middle entry vector for multisite gateway three f…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCMV SOCS-3

    Plasmid

    #11486

    Depositor

    Ronald Kahn

    Article

    Ueki et al Mol Cell Biol. 2004 Jun . 24(12):5434-46

    Insert

    SOCS-3 (Socs3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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48. pkk223_Null

    Plasmid

    #213111

    Purpose

    pkk223 backbone plasmid

    Depositor

    Martin Horvath

    Article

    Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. PLKO.1-UPF1-CDS

    Plasmid

    #136037

    Purpose

    UPF1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (AAGACACCTATTACACGAAGG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. Aiolos in Artichoke

    Plasmid

    #74452

    Purpose

    Monitor post-translational degradation of Aiolos (Lentiviral, SFFV.Aiolos-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

    Depositor

    Benjamin Ebert

    Insert

    Aiolos (IKZF3) (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    SFFV and EF1a

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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