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177,160 results

Showing: 4501 - 4550 of 177160 results

  11. pcDNA3.1-(-)-Pur

    Plasmid
    #200458
    Purpose
    Eukaryotic expression vector
    Depositor
    Bertrand Collet
    Type
    Empty backbone
    Expression
    Mammalian
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    200458-plasmid-map-sequence-id-394118

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  13. pMRX-IB-pSu9-HaloTag7-mGFP

    Plasmid
    #184905
    Purpose
    Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity
    Depositor
    Noboru Mizushima
    Publication
    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Presequence of Fo-ATPase subunit 9
    Tags
    HaloTag7 and monomeric EGFP
    Expression
    Mammalian
    Available since
    June 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    184905-plasmid-map-sequence-id-364106

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  14. Luc-PS9

    Plasmid
    #177942
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luc2 (ORF1ab )
    Expression
    Mammalian
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    177942-plasmid-map-sequence-id-348206

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  15. pET-pAG-Hia5

    Plasmid
    #174373
    Purpose
    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Publication
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein AG - hia5
    Tags
    6xHis and Protein A/G
    Expression
    Bacterial
    Available since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174373-plasmid-map-sequence-id-341861

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  16. pET-PE2-His (IK1822)

    Plasmid
    #170103
    Purpose
    T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS
    Depositor
    Keith Joung
    Publication
    Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103
    Insert
    bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
    Use
    CRISPR
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Bacteria codon-optimized nSpCas9 (H840A) & M-…
    Promoter
    T7
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    170103-plasmid-map-sequence-id-332603

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  18. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid
    #138148
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Publication
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NES
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    138148-plasmid-map-sequence-id-318593

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  21. HDM_SARS2_Spike_del21_D614G

    Plasmid
    #158762
    Purpose
    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614
    Depositor
    Jesse Bloom
    Publication
    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS2-Spike_del21_D614G (S Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    deleted amino acids 1257-1278, changed aspartic a…
    Promoter
    CMV
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    17 Articles
    158762-plasmid-map-sequence-id-309495

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  23. pLVX-EF1alpha-SARS-CoV-2-nsp10-2xStrep-IRES-Puro

    Plasmid
    #141376
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp10 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    10 Articles
    141376-plasmid-map-sequence-id-288095

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  25. pHREAC

    Plasmid
    #134908
    Purpose
    Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.
    Depositor
    George Lomonossoff
    Publication
    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    double 35S
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    134908-plasmid-map-sequence-id-342556

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  31. anxA2-GFP

    Plasmid
    #107196
    Purpose
    Expresses human annexin A2-GFP fusion protein for live cell imaging
    Depositor
    Volker Gerke, Ursula Rescher
    Publication
    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    annexin A2 (ANXA2 Human)
    Tags
    Green Fluorescent Protein
    Expression
    Mammalian
    Mutation
    Ala residue 65 in human sequence replaced by Glu …
    Promoter
    CMV
    Available since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    107196-plasmid-map-sequence-id-209665

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  35. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

    Plasmid
    #92139
    Purpose
    Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection
    Depositor
    Benoit Bruneau
    Publication
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    92139-plasmid-map-sequence-id-349059

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  40. pTT5-hSDC1

    Plasmid
    #52326
    Purpose
    expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Publication
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SDC1 (SDC1 Human)
    Expression
    Mammalian
    Mutation
    R117Q mutation (R95Q according to numbering in Fi…
    Promoter
    CMV
    Available since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    52326-plasmid-map-sequence-id-134443

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  43. pLenti.CAG.H2B-Dendra2.W

    Plasmid
    #51005
    Purpose
    Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-Dendra2
    Use
    Lentiviral
    Tags
    histone2B
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    51005-plasmid-map-sequence-id-490664

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  50. pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

    Plasmid
    #35500
    Purpose
    AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Publication
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    CaMKIIa
    Available since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    35500-plasmid-map-sequence-id-43570

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Showing: 4501 - 4550 of 177160 results