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163,682 results

Showing: 4501 - 4550 of 163682 results

  3. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid
    #140973
    Purpose
    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai1-RLuc8 (GNAI1 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140973-plasmid-map-sequence-id-300655

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  4. TFORF0641

    Plasmid
    #143232
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    THAP11 (THAP11 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143232-plasmid-map-sequence-id-396112

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  6. Ccas9LacZ

    Plasmid
    #113083
    Purpose
    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.
    Depositor
    Tibor Vellai
    Article
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    113083-plasmid-map-sequence-id-218959

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  12. pcDNA3-BG4-NLS-mVen

    Plasmid
    #211449
    Purpose
    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence
    Depositor
    Helmut Pospiech
    Insert
    Anti-DNA G-quadruplex single chain antibody BG4
    Use
    Tags
    NLS-mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211449-plasmid-map-sequence-id-419262

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  13. pSPObooster

    Plasmid
    #216160
    Purpose
    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains
    Depositor
    Grant Brown
    Article
    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978
    Inserts
    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    MKT1 gene containing an aspartic acid to glycine …
    Promoter
    endogenous promoter
    Available Since
    May 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    216160-plasmid-map-sequence-id-433867

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  16. pSelAct-Express

    Plasmid
    #174375
    Purpose
    Plasmid for integrative expression, pUC19 backbone, Apr resistant casette, pCMP1::eGFP expression cassette, gateway compatible
    Depositor
    Gitta Coaker
    Article
    Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(
    Type
    Empty backbone
    Use
    Expression vector for targeted integration
    Tags
    Expression
    Mutation
    Promoter
    pCMP1
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    174375-plasmid-map-sequence-id-370623

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  24. MSCV IRES Luciferase

    Plasmid
    #18760
    Depositor
    Scott Lowe
    Type
    Empty backbone
    Use
    Luciferase and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only
    18760-plasmid-map-sequence-id-169127

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  33. pWPT-nlsLacZ

    Plasmid
    #12261
    Depositor
    Didier Trono
    Insert
    LacZ
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12261-plasmid-map-sequence-id-7273

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  35. pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG

    Plasmid
    #70085
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2
    Insert
    mutated human serum transferrin (TF Human)
    Use
    Tags
    Hexa His tag and N-terminal signal peptide, 4 aa …
    Expression
    Mammalian
    Mutation
    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426…
    Promoter
    SV40
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70085-plasmid-map-sequence-id-129388

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  39. AICSDP-10:LMNB1-mEGFP

    Plasmid
    #87422
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available Since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87422-plasmid-map-sequence-id-173949

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  43. pLEX-307_LgBiT

    Plasmid
    #191211
    Purpose
    Lentiviral-mediated stable expression of LgBiT for slit nanoluciferase complementation assays in mammalian cells
    Depositor
    Mohan Babu
    Insert
    LgBiT (large bit)
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mutation
    AB030246.1 (mutated in PMID 26569370)
    Promoter
    Available Since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191211-plasmid-map-sequence-id-400915

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  45. pHAGE-CMV-Flag-TLK2_IDG-K

    Plasmid
    #135278
    Purpose
    Gateway destination clone of TLK2 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Ben Major
    Insert
    Flag-TLK2 (TLK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    135278-plasmid-map-sequence-id-267204

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  50. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid
    #200408
    Purpose
    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    iRFP720 (frameshift mutant)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200408-plasmid-map-sequence-id-396809

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Showing: 4501 - 4550 of 163682 results