20,421 results
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Plasmid#140829PurposeAIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757DepositorInsertAIMTOR A757 (ULK1 Human)
UseTagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…PromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
alpha Synuclein-VC
Plasmid#89471Purposeexpresses human alpha Synuclein fused to the C-terminal part of VenusDepositorInsertSNCA (SNCA Human)
UseTagsC-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFEB promoter-luciferase reporter
Plasmid#66801PurposeReporter for activity of TFEB gene promoterDepositorInsertTFEB promoter (Tfeb Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUG-T2A-cre
Plasmid#66691PurposeRecombine floxed alleles in any cellDepositorInsertGFP-T2A-Cre
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7
Plasmid#11795Purpose3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-tetOn-IRES-G418 (EtO)
Plasmid#84776PurposeSecond generation lenti tetracycline operator (on)DepositorInsertTET OPERATOR
UseLentiviralTagsExpressionMutationPromoterpTIGHTAvailable sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro FLAG SREBP2
Plasmid#32018DepositorInsertSREBP2 (Srebf2 Mouse)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR-tdPP7-3xmCherry
Plasmid#74926PurposeExpresses tdPP7-3xmCherryDepositorInsertPP7-3xmCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-hPKM1
Plasmid#44241DepositorInsertPyruvate Kinase M1 (PKM Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
U6-5'+3' MS2-CircRNA
Plasmid#213752PurposeFor circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cellsDepositorInsert5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRE:GFP
Plasmid#163036PurposeFLiCRE reporter gene for neuronal expressionDepositorInsertGFP
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ubc.Luc.IRES.Puro
Plasmid#33307DepositorInsertFirefly Luciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterUbcAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pC0040-LwaCas13a crRNA backbone
Plasmid#103851PurposeBackbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAADepositorInsertU6-LwaCas13aDR-BbsI-BbsI-polyT
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB-rtTA
Plasmid#126034PurposePiggyBac cargo vector expressing rtTA. Also confers resistance to G418.DepositorInsertrtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
UsePiggybacTagsExpressionMammalianMutationPromoterhUbiCAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 humanTRAF6 (1090)
Plasmid#66930PurposeMammalian Expression of TRAF6DepositorInsertTRAF6 (TRAF6 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-Halo7Tag-hFis
Plasmid#111136PurposehFis with selflabeling tag, e.g. for fluorescence microscopyDepositorInserthuman fission factor1 (FIS1 Human)
UseTagsHalo7-tagExpressionMammalianMutation*PromoterCMVAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA Seh1L pRK5
Plasmid#46331DepositorInsertSeh1 (SEH1L Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCR8 JARID2
Plasmid#114443PurposeEntry vector for gateway cloning containing human JARID2 coding sequenceDepositorInsertJARID2 (JARID2 Human)
UseGateway entry cloningTagsExpressionMutationPromoterNoneAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-hPIASx alpha
Plasmid#15209DepositorInsertPIASx alpha (PIAS2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-CUL4B
Plasmid#19922DepositorInsertCullin 4B (CUL4B Human)
UseTags3xmycExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pC3.1-caggs-Crimson-CAAX
Plasmid#190041PurposeRed Fluorescent Protein for Long-Term Labeling of Fine Structures in NeuronsDepositorInsertCrimson
UseTagsCAAXExpressionMammalianMutationPromotercaggsAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 (AAV1)
Viral Prep#98927-AAV1PurposeReady-to-use AAV1 particles produced from pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 (#98927). In addition to the viral particles, you will also receive purified pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent expression of GFPsm_myc spaghetti monster construct. Spaghetti monster constructs are not fluorescent ("dark") to allow full range of antibody staining. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPsm-myc (spaghetti monster construct)Available sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAL113
Plasmid#22471PurposeExpresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.DepositorInsertPhyB NT (PHYB Mustard Weed, Mouse)
UseSynthetic BiologyTags10aaLinker-mCFP-HrasCTExpressionMammalianMutationY276HPromoterCMVAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAM5505
Plasmid#132664PurposepRL623-based helper plasmid for conjugations, with RSF1010 mobA geneDepositorInsertMobA
UseTagsExpressionBacterialMutationplease see depositor comments belowPromoterp1,p3Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-M2L
Plasmid#20926Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28DepositorArticleUseEpisomal/ebnaTagsExpressionMutationPromoterCMV and IRESAvailable sinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCI-EGFP-NR1 wt
Plasmid#45446DepositorInsertNR1 (Grin1 Rat)
UseTagsEGFPExpressionMammalianMutationEGFP inserted after the predicted signal peptide …PromoterCMVAvailable sinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJG103
Plasmid#158533PurposewrmScarlet11 x3 tandem repeatsDepositorInsertwrmScarlet11 x3 tandem repeats
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK344 (Hygro-P2A-mAID)
Plasmid#121178PurposeN-terminal taggingDepositorInsertHygro-P2A-mAID
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
LZRS-DNMT1
Plasmid#24952DepositorInsertDNMT1 (DNMT1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
CIBN-GFP-Sec61B
Plasmid#104177PurposeExpresses fusion of CIB1 (1-170) with GFP and Sec61BDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-PSMD14
Plasmid#22557DepositorInsertPSMD14 (PSMD14 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-MK1-EHMT2-V5
Plasmid#31113DepositorInserteuchromatic histone-lysine N-methyltransferase 2 (EHMT2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationStop codon mutated to AlaPromoterAvailable sinceAug. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_LaG16_2_synNotch_Gal4VP64
Plasmid#85421PurposeLentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic NotchDepositorInsertLaG16_2 GFP nanobody synNotch Gal4VP64
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIP sHRPa-Aga1P
Plasmid#73151PurposeLarge fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.DepositorInsertsHRPa-Aga1P
UseTagsV5 tagExpressionYeastMutationPromoterglyceraldehyde-3-phosphate dehydrogenase GPD prom…Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
T4 td upstream intron-PABP
Plasmid#189972PurposePart 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAsDepositorInsertT4 td upstream intron-PABP
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cerulean-N1
Plasmid#54742PurposeLocalization: N1 Cloning Vector, Excitation: 433, Emission: 475DepositorTypeEmpty backboneUseTagsCeruleanExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL4.20-HIF1A prom
Plasmid#40173DepositorInsertHIF1A promoter fragment (HIF1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hCas9
Plasmid#51142PurposeExpresses hCas9 under the CAG promoter for CRISPRDepositorInserthCas9
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCh-KIF5B*-strep
Plasmid#120164PurposeExpresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidinDepositorInsertkinesin family member 5B (Kif5b Mouse)
UseTagsStreptavidin and mCherryExpressionMammalianMutationTruncated KIF5B: 1-555 aaPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC-E75
Bacterial Strain#115925PurposeExpression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosomeDepositorBacterial ResistanceNoneAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEP-GluA2 TKIT
Plasmid#169442PurposeExpression of 2 guides + donor DNADepositorInsertSuper ecliptic pHluorin (SEP)
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PRExpress
Plasmid#122486PurposeA non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)DepositorInsertsLacZ
poly-PRE
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
ClopHensorN
Plasmid#50758PurposeMammalian expression vector expressing the ClopHensorN chloride and proton sensorDepositorInsertClopHensorN
UseTagsStrep-IIExpressionMammalianMutationE2GFP is EGFP-T203YPromoterCAGAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV CMV-TERRA
Plasmid#111375PurposeTERRA expression contains approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequencesDepositorInsertTERRA polyA
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
hAAVS1 TALEN Left
Plasmid#52341PurposeTALEN vector for genomic targetingDepositorInsertAAVS1 TALEN Left
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
CaV2.1 (pcDNA6)
Plasmid#26578DepositorInsertCacna1a (Cacna1a Mouse)
UseTagsHis6 and V5ExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCIRCgp41-1
Plasmid#74227PurposeVector for SICLOPPS-mediated protein circularization using the gp41-1 intein.DepositorTypeEmpty backboneUseSynthetic BiologyTagsgp41-1 C-intein and gp41-1 N-inteinExpressionBacterialMutationPromoterT7-lacAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRE-ChrimsonR-mCherry
Plasmid#92207PurposeReporter constructDepositorInsertChimsonR-mCherry
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-EGFP-WPREpA
Plasmid#84469PurposepAAV-RAM-EGFPDepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterTREAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19 - T7 pro - IRES - EGFP
Plasmid#138586PurposeExpress EGFP with an upstream T7 promoter in pUC19 backboneDepositorInsertEGFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only