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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,163 results for: GRN

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Showing: 7851 - 7900 of 15163 results

  2. pBM019

    Plasmid
    #128179
    Purpose
    Construct for generating stable Cas9-expressing Toxoplasma gondii
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003
    Inserts
    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Promoter
    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…
    Available Since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    128179-plasmid-map-sequence-id-249095

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  3. PMXS-GOT1

    Plasmid
    #72872
    Purpose
    The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    GOT1 (GOT1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    sgRNA resistant human GOT1, 7 silent mutations c1…
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  7. TMPRSS2 g1 + Cas9

    Plasmid
    #153015
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153015-plasmid-map-sequence-id-298676

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  20. pResQ shFEN3 3XF-FEN1 wt

    Plasmid
    #17752
    Depositor
    Sheila Stewart
    Insert
    Flap Endonuclease I (FEN1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Triple Flag
    Expression
    Mammalian
    Available Since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  24. pLKO-FOXM1-shRNA-84

    Plasmid
    #89494
    Purpose
    Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.
    Depositor
    Adam Karpf
    Insert
    FOXM1 (FOXM1 Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    89494-plasmid-map-sequence-id-180323

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  29. MSP2253

    Plasmid
    #70704
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70704-plasmid-map-sequence-id-127764

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  39. pADH119

    Plasmid
    #90985
    Purpose
    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    gRNA conserved, C. albicans LEU2 2 of 2
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90985-plasmid-map-sequence-id-180513

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  40. pAL-pyk_NT

    Plasmid
    #74072
    Purpose
    pAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pyk (NT)
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    ptac
    Available Since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74072-plasmid-map-sequence-id-314169

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  43. pAL-pgi_NT

    Plasmid
    #74067
    Purpose
    pAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pgi (NT)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    ptac
    Available Since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74067-plasmid-map-sequence-id-405024

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Showing: 7851 - 7900 of 15163 results