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35,044 results

Showing: 8201 - 8250 of 35044 results

  2. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-99784

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  3. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-99784

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  4. pCW57.1 N-term GFP tTA

    Plasmid
    #107551
    Purpose
    All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.
    Depositor
    David Sabatini
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP (optional)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107551-plasmid-map-sequence-id-208339

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  7. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Article
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Insert
    RNASEH1 (RNASEH1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Promoter
    Available since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    111905-plasmid-map-sequence-id-212872

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  10. InsRA-eGFP

    Plasmid
    #79795
    Purpose
    Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
    Depositor
    James Johnson
    Article
    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:
    Insert
    InsRA (INSR Human)
    Use
    Tags
    eGFP extracellular
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    79795-plasmid-map-sequence-id-149406

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  11. InsRA-eGFP

    Plasmid
    #79795
    Purpose
    Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
    Depositor
    James Johnson
    Article
    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:
    Insert
    InsRA (INSR Human)
    Use
    Tags
    eGFP extracellular
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    79795-plasmid-map-sequence-id-149406

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  13. pCAG-VSVL

    Plasmid
    #64085
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene
    Depositor
    Ian Wickersham
    Insert
    VSV polymerase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64085-plasmid-map-sequence-id-429028

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  14. pCAG-VSVL

    Plasmid
    #64085
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene
    Depositor
    Ian Wickersham
    Insert
    VSV polymerase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64085-plasmid-map-sequence-id-429028

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  17. dRED

    Plasmid
    #139836
    Purpose
    Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Use
    Tags
    Expression
    Mammalian
    Mutation
    RNase H1 protein contains D210N mutation. dCas9 …
    Promoter
    tet-inducible CMV
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139836-plasmid-map-sequence-id-308228

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  31. mEGFP-H4-23

    Plasmid
    #56463
    Purpose
    Localization: Nucleus/Histones, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Insert
    H4 (HIST1H4J Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    56463-plasmid-map-sequence-id-244945

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  32. mEGFP-H4-23

    Plasmid
    #56463
    Purpose
    Localization: Nucleus/Histones, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Insert
    H4 (HIST1H4J Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    56463-plasmid-map-sequence-id-244945

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  33. HTR7-Tango

    Plasmid
    #66415
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HTR7 (HTR7 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66415-plasmid-map-sequence-id-251911

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  34. HTR7-Tango

    Plasmid
    #66415
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HTR7 (HTR7 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66415-plasmid-map-sequence-id-251911

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  35. mEos3.2-Lifeact-7

    Plasmid
    #54696
    Purpose
    Localization: Actin, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mEos3.2-Lifeact
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54696-plasmid-map-sequence-id-137746

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  36. mEos3.2-Lifeact-7

    Plasmid
    #54696
    Purpose
    Localization: Actin, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mEos3.2-Lifeact
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54696-plasmid-map-sequence-id-137746

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  45. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

    Plasmid
    #111731
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q145 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    111731-plasmid-map-sequence-id-226895

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  46. pT3-EF1A-MYC-IRES-lucOS

    Plasmid
    #129776
    Purpose
    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339
    Depositor
    Amaia Lujambio
    Article
    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82
    Insert
    human MYC (MYC Human)
    Use
    Tags
    OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    129776-plasmid-map-sequence-id-263368

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Showing: 8201 - 8250 of 35044 results