Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

292,695 results

Showing: 8301 - 8350 of 292695 results

  1. cytoplasmic NAD+ biosensor

    Plasmid
    #186787
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm
    Depositor
    Michael Cohen
    Article
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    cytoplasmic NAD+ biosensor
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186787-plasmid-map-sequence-id-368056

    Enlarge

  2. cytoplasmic NAD+ biosensor

    Plasmid
    #186787
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm
    Depositor
    Michael Cohen
    Article
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    cytoplasmic NAD+ biosensor
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186787-plasmid-map-sequence-id-368056

    Enlarge

  10. pSIN-TRE-EB3N-VVDfast-mCl3-iLID

    Plasmid
    #190169
    Purpose
    Doxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60.
    Depositor
    Anna Akhmanova
    Article
    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e
    Insert
    EB3N-VVDfast-mClover3-iLID
    Use
    Lentiviral
    Tags
    -VVDfast-mClover3-iLID
    Expression
    Mutation
    1-189 aa from EB3
    Promoter
    TRE
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190169-plasmid-map-sequence-id-373726

    Enlarge

  11. pSIN-TRE-EB3N-VVDfast-mCl3-iLID

    Plasmid
    #190169
    Purpose
    Doxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60.
    Depositor
    Anna Akhmanova
    Article
    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e
    Insert
    EB3N-VVDfast-mClover3-iLID
    Use
    Lentiviral
    Tags
    -VVDfast-mClover3-iLID
    Expression
    Mutation
    1-189 aa from EB3
    Promoter
    TRE
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190169-plasmid-map-sequence-id-373726

    Enlarge

  12. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

    Enlarge

  13. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

    Enlarge

  18. pAAV-mDlx-NLS-mRuby2 (AAV9)

    Viral Prep
    #99130-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-mDlx-NLS-mRuby2 (#99130). In addition to the viral particles, you will also receive purified pAAV-mDlx-NLS-mRuby2 plasmid DNA. mDlx-driven NLS-mRuby2 expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Article
    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.
    Promoter
    mDLX5/6
    Tags
    mRuby2
    Available since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  19. pMal-T-Avi-His/BirA

    Plasmid
    #102962
    Purpose
    Vector for in vivo biotinylation of a fusion protein in bacteria.
    Depositor
    Tonia Rex
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    102962-plasmid-map-sequence-id-199098

    Enlarge

  20. pMal-T-Avi-His/BirA

    Plasmid
    #102962
    Purpose
    Vector for in vivo biotinylation of a fusion protein in bacteria.
    Depositor
    Tonia Rex
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    102962-plasmid-map-sequence-id-199098

    Enlarge

  21. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV2)

    Viral Prep
    #26969-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.
    Promoter
    CaMKII
    Tags
    EYFP
    Available since
    Feb. 14, 2018
    Availability
    Academic Institutions and Nonprofits only

  32. pLenti-C-Myc-DDK-IRES-Puro HEXIM1

    Plasmid
    #200726
    Purpose
    Lentiviral expression of Flag-Tagged human HEXIM1
    Depositor
    Claudio Alarcón
    Insert
    HEXIM1 (HEXIM1 Human)
    Use
    Lentiviral
    Tags
    Myc-DDK from backbone plasmid
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    200726-plasmid-map-sequence-id-401797

    Enlarge

  34. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Article
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U1a and U6
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112139-plasmid-map-sequence-id-217823

    Enlarge

  35. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Article
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U1a and U6
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112139-plasmid-map-sequence-id-217823

    Enlarge

  42. pAAV-hSynapsin1-RdLight1 (AAV9)

    Viral Prep
    #125708-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSynapsin1-RdLight1 (#125708). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-RdLight1 plasmid DNA. Synapsin-driven expression of red genetically encoded dopamine sensor RdLight1. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.
    Promoter
    Syn
    Tags
    None
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only

  43. pQCXIB (w297-1)

    Plasmid
    #22800
    Depositor
    Eric Campeau
    Type
    Empty backbone
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 17, 2009
    Availability
    Academic Institutions and Nonprofits only
    22800-plasmid-map-sequence-id-10440

    Enlarge

  44. pQCXIB (w297-1)

    Plasmid
    #22800
    Depositor
    Eric Campeau
    Type
    Empty backbone
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 17, 2009
    Availability
    Academic Institutions and Nonprofits only
    22800-plasmid-map-sequence-id-10440

    Enlarge

  47. c-Flag pcDNA3

    Plasmid
    #20011
    Purpose
    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.
    Depositor
    Stephen Smale
    Article
    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20011-plasmid-map-sequence-id-372933

    Enlarge

  48. c-Flag pcDNA3

    Plasmid
    #20011
    Purpose
    This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.
    Depositor
    Stephen Smale
    Article
    Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20011-plasmid-map-sequence-id-372933

    Enlarge

Showing: 8301 - 8350 of 292695 results