Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

305,743 results

Showing: 801 - 850 of 305743 results

  8. pLV CMV IGF1-FLAG

    Plasmid
    #175170
    Purpose
    Lentiviral expression of human Igf1-FLAG
    Depositor
    Larry Gerace
    Insert
    Igf1 (IGF1 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    175170-plasmid-map-sequence-id-352639

    Enlarge

  9. pLV CMV IGF1-FLAG

    Plasmid
    #175170
    Purpose
    Lentiviral expression of human Igf1-FLAG
    Depositor
    Larry Gerace
    Insert
    Igf1 (IGF1 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    175170-plasmid-map-sequence-id-352639

    Enlarge

  16. MYST3

    Plasmid
    #25181
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MYST3_13 (KAT6A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains catalytic region ( residues 497-780).
    Promoter
    Available since
    Oct. 25, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. MYST3

    Plasmid
    #25181
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MYST3_13 (KAT6A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains catalytic region ( residues 497-780).
    Promoter
    Available since
    Oct. 25, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pD649-HAsp-NCAM1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156948
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    NCAM1 (NCAM1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156948-plasmid-map-sequence-id-412906

    Enlarge

  21. pD649-HAsp-NCAM1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156948
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    NCAM1 (NCAM1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156948-plasmid-map-sequence-id-412906

    Enlarge

  24. pLEX307-FURIN-G418

    Plasmid
    #158462
    Purpose
    Constitutive lentiviral expression of human FURIN.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    FURIN (FURIN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158462-plasmid-map-sequence-id-308175

    Enlarge

  25. pLEX307-FURIN-G418

    Plasmid
    #158462
    Purpose
    Constitutive lentiviral expression of human FURIN.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    FURIN (FURIN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158462-plasmid-map-sequence-id-308175

    Enlarge

  28. TFORF2865

    Plasmid
    #144159
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    WIZ (WIZ Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144159-plasmid-map-sequence-id-396221

    Enlarge

  29. TFORF2865

    Plasmid
    #144159
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    WIZ (WIZ Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144159-plasmid-map-sequence-id-396221

    Enlarge

  30. 14UAS PSD95:GFP 5UAS DSRedExpress

    Plasmid
    #74315
    Purpose
    Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish
    Depositor
    Martin Meyer, Stephen Smith
    Article
    Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub
    Inserts
    zebrafish PSD95
    DsRedExpress
    Use
    Tags
    EGFP
    Expression
    Mutation
    Y599H and I793S (please see depositor comment bel…
    Promoter
    Available since
    May 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  31. 14UAS PSD95:GFP 5UAS DSRedExpress

    Plasmid
    #74315
    Purpose
    Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish
    Depositor
    Martin Meyer, Stephen Smith
    Article
    Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub
    Inserts
    zebrafish PSD95
    DsRedExpress
    Use
    Tags
    EGFP
    Expression
    Mutation
    Y599H and I793S (please see depositor comment bel…
    Promoter
    Available since
    May 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  32. pCS2_hMGFP-60A

    Plasmid
    #200393
    Purpose
    A plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.
    Depositor
    Xiao Wang
    Article
    Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3
    Insert
    monster GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    200393-plasmid-map-sequence-id-392993

    Enlarge

  33. pCS2_hMGFP-60A

    Plasmid
    #200393
    Purpose
    A plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.
    Depositor
    Xiao Wang
    Article
    Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3
    Insert
    monster GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    200393-plasmid-map-sequence-id-392993

    Enlarge

  39. Desmoplakin I tension sensor (F40-based)

    Plasmid
    #119186
    Purpose
    The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119186-plasmid-map-sequence-id-230533

    Enlarge

  40. Desmoplakin I tension sensor (F40-based)

    Plasmid
    #119186
    Purpose
    The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119186-plasmid-map-sequence-id-230533

    Enlarge

  43. pLIX403_deltaUBA_UBAP2L_mCherry

    Plasmid
    #105287
    Purpose
    puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2L
    Depositor
    Eugene Yeo
    Article
    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do
    Insert
    UBAP2L (UBAP2L Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deleted amino acids 20-108
    Promoter
    TRE promoter, Tet ON
    Available since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    105287-plasmid-map-sequence-id-223527

    Enlarge

  44. pLIX403_deltaUBA_UBAP2L_mCherry

    Plasmid
    #105287
    Purpose
    puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2L
    Depositor
    Eugene Yeo
    Article
    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do
    Insert
    UBAP2L (UBAP2L Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deleted amino acids 20-108
    Promoter
    TRE promoter, Tet ON
    Available since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    105287-plasmid-map-sequence-id-223527

    Enlarge

  47. pRF0G-Tyr

    Plasmid
    #113644
    Purpose
    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    MjYRS and tRNA gene
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    113644-plasmid-map-sequence-id-217129

    Enlarge

  48. pRF0G-Tyr

    Plasmid
    #113644
    Purpose
    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    MjYRS and tRNA gene
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    113644-plasmid-map-sequence-id-217129

    Enlarge

Showing: 801 - 850 of 305743 results