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161,398 results

Showing: 801 - 850 of 161398 results

  1. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid
    #72325
    Purpose
    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a SnoopTag-mEGFP-SpyTag
    Use
    Tags
    N-terminal His6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72325-plasmid-map-sequence-id-137259

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  10. pLenti-AncBE4max

    Plasmid
    #174164
    Purpose
    This is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.
    Depositor
    Wensheng Wei
    Article
    Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103
    Insert
    AncBE4max
    Use
    Lentiviral
    Tags
    P2A-mCherry
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174164-plasmid-map-sequence-id-342437

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  17. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid
    #208963
    Purpose
    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    EcKlenow-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208963-plasmid-map-sequence-id-415798

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  19. pENN.AAV.CamKII 0.4.Cre.SV40 (AAV9)

    Viral Prep
    #105558-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CaMKII
    Tags
    None
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  21. pFP.E227

    Plasmid
    #138249
    Purpose
    Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    mCherry
    Terminator
    mTagBFP
    Terminator
    gfp
    Terminator
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P17_up1691, P20_992, and iP16_3622
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138249-plasmid-map-sequence-id-273529

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  24. pLenti-mCherry-CAAX

    Plasmid
    #129285
    Purpose
    Lentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence
    Depositor
    Carlos Carmona-Fontaine
    Article
    Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2
    Insert
    Fluorescent reporter mCherry fused to CAAX polybasic sequence
    Use
    Lentiviral
    Tags
    CAAX
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    129285-plasmid-map-sequence-id-252373

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  35. pExp-MsyB

    Plasmid
    #112570
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    112570-plasmid-map-sequence-id-255699

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  41. qTAG-N-Solo-mStayGold-TJP1

    Plasmid
    #227300
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227300-plasmid-map-sequence-id-451538

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  46. pFP.E222

    Plasmid
    #138248
    Purpose
    Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    ECF20.N.(VDA).M86.N2
    Terminator
    SspGyrB.C2.(SAK).ECF16.C
    Terminator
    rhaS
    ECF17.N.(NPC).NrdJ-1.N2
    Terminator
    M86.C2.(SDL).ECF20.C
    Terminator
    luxR
    Terminator
    ECF16.N.(AGG).SspGyrB.N2
    Terminator
    NrdJ-1.C2.(SEI).ECF17.C
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138248-plasmid-map-sequence-id-273451

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  48. pLXRN-EGFP

    Plasmid
    #99207
    Purpose
    EGFP expressing bicistronic retroviral vector
    Depositor
    Peter Pedersen
    Insert
    EGFP
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    A Kozak sequence was incorporated to enhance tran…
    Promoter
    LTR
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99207-plasmid-map-sequence-id-190957

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Showing: 801 - 850 of 161398 results