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Addgene

We narrowed to 6,120 results for: nls

Showing: 901 - 950 of 6120 results
  1. R4pGWB601_UBQ10p-miniTurbo-YFP-NLS

    Plasmid
    #127370
    Purpose
    Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants
    Depositor
    Insert
    miniTurbo (BirA mutant)
    Use
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Plant
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid
    #117699
    Purpose
    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA
    Depositor
    Inserts
    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    Use
    Tags
    HA tag, Myc-NES, and NLS
    Expression
    Mammalian
    Mutation
    codon optimized for murine expression
    Promoter
    None, 2A peptide from porcine teschovirus-1 polyp…
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pCMV-dCas13-inactive M3nls

    Plasmid
    #157854
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    Insert
    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Promoter
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3-FKBP-ICUE3-NLS

    Plasmid
    #138255
    Purpose
    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Insert
    FKBP-ICUE3-NLS
    Use
    Tags
    FKBP, Nuclear localization signal (NLS), cpVenus[…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 31, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3-BG4-NLS-mCer

    Plasmid
    #211448
    Purpose
    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence
    Depositor
    Insert
    Anti-DNA G-quadruplex single chain antibody BG4
    Use
    Tags
    NLS-mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pET-MBP-NLS-Geo_st

    Plasmid
    #87703
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS
    Depositor
    Insert
    GeoCas9
    Use
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. dCas9-NS3-NLS/VPR

    Plasmid
    #112244
    Purpose
    Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.
    Depositor
    Insert
    dCas9-NS3-NLS/VPR
    Use
    Tags
    AU1 (N term on NS3) and HA (C term on NS3)
    Expression
    Mammalian
    Mutation
    NS3 mutant T54A
    Promoter
    CMV
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pUBC-SV40NLS-Pfv-Sapphire

    Plasmid
    #203653
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.
    Depositor
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 901 - 950 of 6120 results