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179,539 results

Showing: 10051 - 10100 of 179539 results

  1. FRB-mCherry-FTH1

    Plasmid
    #100749
    Purpose
    Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag
    Depositor
    Stephen Royle
    Publication
    Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ferritin heavy chain 1 (FTH1 Human)
    Tags
    FRB and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    100749-plasmid-map-sequence-id-194021

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  2. pX330a dCas9-KRAB

    Plasmid
    #92361
    Purpose
    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KRAB
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    92361-plasmid-map-sequence-id-178231

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  3. AAV pCAG-FLEX-mScarlet-WPRE

    Plasmid
    #99280
    Purpose
    Can be used to generate AAV virus that will express mScarlet in the presence of Cre
    Depositor
    Rylan Larsen
    Insert
    mScarlet
    Use
    AAV and Cre/Lox
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    99280-plasmid-map-sequence-id-487711

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  4. AICSDP-35:AAVS1-mEGFP

    Plasmid
    #91565
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 7, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    3 Articles
    91565-plasmid-map-sequence-id-456463

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  9. ATP1A1_plasmid_donor_RD

    Plasmid
    #86551
    Purpose
    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 (ATP1A1 Human)
    Use
    CRISPR and TALEN; Co-selection via hdr using ouab…
    Expression
    Mammalian
    Mutation
    Q118R, N129D
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    86551-plasmid-map-sequence-id-171421

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  10. pCMV-SPORT6-hHMGCR1

    Plasmid
    #86085
    Purpose
    expresses the human HMG-CoA reductase isoform 1
    Depositor
    Anne Galy
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human HMG-CoA Reductase isoform 1 (HMGCR Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles

  15. pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9

    Plasmid
    #84240
    Purpose
    Expresses GA-inducible VPR-Sp dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sp dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    84240-plasmid-map-sequence-id-160812

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  17. Grp1-PH pEGFP-C1

    Plasmid
    #71378
    Purpose
    For analysis of PH domain localization in mammalian cells
    Depositor
    Mark Lemmon
    Publication
    Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Grp1-PH (Cyth3 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Pleckstrin homology (PH) domain; aa 247-399
    Promoter
    CMV
    Available since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    71378-plasmid-map-sequence-id-422685

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  18. p5E-gfap

    Plasmid
    #75024
    Purpose
    Can be used to drive expression in zebrafish astrocytes.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    glial fibrillary acidic protein promoter (gfap Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    gfap
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    75024-plasmid-map-sequence-id-450679

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  26. alpha catenin conformation sensor

    Plasmid
    #71709
    Purpose
    This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.
    Depositor
    Deborah Leckband
    Publication
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha-catenin FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71709-plasmid-map-sequence-id-142365

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  29. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Publication
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    pBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  32. pHBV-Luc

    Plasmid
    #71414
    Purpose
    To see the activity of HBV transcription using Luciferase assay
    Depositor
    Wang-Shick Ryu
    Publication
    Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HBV core promoter, enhancer I, enhancer II and luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    HBV
    Available since
    Dec. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    71414-plasmid-map-sequence-id-132686

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  35. p5E-mfap4

    Plasmid
    #70052
    Purpose
    5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.
    Depositor
    David Tobin
    Publication
    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do
    Insert
    mfap4 promoter
    Use
    Gateway 5' element
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70052-plasmid-map-sequence-id-130081

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  37. Str-KDEL_ManII-SBP-EGFP

    Plasmid
    #65252
    Purpose
    synchronize trafficking of ManII from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptavidin-KDEL and truncated Mannosidase II
    Tags
    EGFP and Streptavidin Binding Protein (SBP)
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    65252-plasmid-map-sequence-id-120582

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  43. TGFB1-bio-His

    Plasmid
    #52185
    Purpose
    Expresses full-length Transforming growth factor beta-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TGFB1 (TGFB1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    52185-plasmid-map-sequence-id-80549

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  44. pBUN421

    Plasmid
    #62204
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    TaU3 and Ubi
    Available since
    Feb. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    62204-plasmid-map-sequence-id-101817

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  45. pSBbi-BP

    Plasmid
    #60512
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and puromycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    60512-plasmid-map-sequence-id-97534

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  50. FRB_SCF2_LD6_CTEV

    Plasmid
    #58879
    Purpose
    MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease
    Depositor
    Joshua Leonard
    Publication
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58879-plasmid-map-sequence-id-94240

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Showing: 10051 - 10100 of 179539 results