164,909 results
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GFP-polyQ23-NLS
Plasmid#231891PurposeExpression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleusDepositorInsertHTT partial exon 1 Q23-NLS (HTT Human)
UseTagsEGFP and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTomo
Plasmid#26291DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W
Plasmid#67980PurposeCas9 activity reporter with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-15b_LC3B
Plasmid#73949PurposeFor recombinant expression of human LC3B/MAP1LC3B in E. coliDepositorInsertLC3B (MAP1LC3B Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-Cdc42-2G
Plasmid#68814PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)DepositorInsertCdc42 activation reporter
UseTagsHis and MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF1652
Plasmid#143532PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSPI1 (SPI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
IL-2 promoter luciferase
Plasmid#12194DepositorInsertIL-2 promoter (Il2 Mouse)
UseLuciferaseTagsluciferaseExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
PX856
Plasmid#62888PurposeC-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)DepositorInsertSpCas9 (aa536-1368)
UseCRISPRTagsFKBP12, NLS, NLS SV40, and VP64ExpressionMammalianMutationAsparagine 863 to Alanine (N863A)PromoterCBhAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcs2-GFP-IRES-mCherry
Plasmid#226098PurposeProtein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas9-recX
Plasmid#158715PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recXDepositorInsertcas9
UseCRISPRTagsExpressionBacterialMutationPromoterTetOAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA AKT1
Plasmid#78778PurposeTo overexpress AKT1 in Mammalian CellsDepositorInsertAKT1 (AKT1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHIV_pTp66p51
Plasmid#78233PurposeExpresses HIV reverse transcriptaseDepositorInsertsp66
p51
LacI
UseTagsExpressionBacterialMutationPromoterConst. Promoter (From DNA2.0 Vec) and pTac Minima…Available sinceAug. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-6xNLS-E174R/S542R (pRG232)
Plasmid#149723PurposeLentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
IRE1 alpha-pcDNA3.EGFP
Plasmid#13009DepositorInsertInositol Requiring Enzyme-1 (ERN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
TFORF2069
Plasmid#141902PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pU6-PspCas13b-GEMSgRNA2
Plasmid#204768PurposepU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.DepositorInsertguide RNA targeting GEMS m6A sensor mRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pN7-S15
Plasmid#190665PurposeExpresses N7 ribosomal protein S15DepositorInserthuman codon optimized N7 S15
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE25
Plasmid#90367PurposeNotch - NIDC/CBF1 gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterNotchAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBADmod1-linker2-gamS
Plasmid#45833DepositorInsertgamS
UseSynthetic BiologyTagsHistagExpressionBacterialMutationPromoterpBADAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBR BsmAIM Kan + p15A BsaIR Amp
Plasmid#206208PurposeContains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIRDepositorInsertBsamAIM and BsaIR
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
ISceI-GR-RFP
Plasmid#17654DepositorUseTagsmRFPExpressionMammalianMutationC655G mutation in the glucocorticoid receptor (se…PromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDESTmycYBX1
Plasmid#19878DepositorInsertY box binding protein 1 (YBX1 Human)
UseTagsMYCExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCpf1b-sp
Plasmid#122188PurposeBacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr-mKO-MK2
Plasmid#115492PurposeExpression vector for the p38 activity reporter mKO-MK2 in mammalian cellsDepositorInsertmKO-MK2 (MAPKAPK2 Human)
UseTagsmonomeric Kusabira Orange (mKO)ExpressionMammalianMutationPromoterCAGAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
RFP-PASS
Plasmid#193971PurposeMonitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)DepositorInsertPA biosenor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1-PinkyCaMP
Plasmid#232861PurposeA vector expressing the mScarlet based calcium indicator PinkyCaMP under CMV promoterDepositorInsertPinkyCaMP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC
Plasmid#62806PurposeTo express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.DepositorTypeEmpty backboneUseAAV; EucaryoticTagsExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
mApple-TOMM20-N-10
Plasmid#54955PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 568, Emission: 592DepositorInsertTOMM20 (TOMM20 Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-hIFITM1
Plasmid#58399PurposeExpressed human IFITM1 with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM1 (IFITM1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
SP1-SB100X
Plasmid#154887PurposeSleeping Beauty transposase fused to SP1DepositorInsertSP1-Sleeping Beauty
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-dual-CRISPR-U6-H1(pBP43)
Plasmid#218930PurposeThe backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6 and H1Available sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-wtATMSPARK2
Plasmid#195247Purposeexpress wtATMSPARK2DepositorInsertATMSPARK2 sensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-tdMCP-YFP
Plasmid#99151PurposeExpresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vectorDepositorInsertDimeric MS2-coat binding protein
UseLentiviralTagseYFPExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-S33Y Beta-catenin
Plasmid#19286PurposeMammalian expression of human beta-catenin with Flag tag and S33Y mutationDepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsFlagExpressionMammalianMutationS33Y (presumptive GSK3β phosphorylation site)PromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
856 pBabe puroL PTEN C124S
Plasmid#10931DepositorInsertPTEN (PTEN Human)
UseRetroviralTagsExpressionMammalianMutationC124SPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV8)
Viral Prep#104492-AAV8PurposeReady-to-use AAV8 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1a-Bmi1
Plasmid#31783DepositorInsertBmi1 (BMI1 Human)
UseTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)
Plasmid#225754PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC-SP6
Plasmid#102418PurposeAc transposase with SP6 promoter for in vitro transcriptionDepositorInsertAc transposase
UseTagsExpressionBacterialMutationPromoterSP6Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET-4xTR-TUBE
Plasmid#110312PurposeE. coli expression and purification of 4xTR-TUBEDepositorInsert4xTR-TUBE (UBQLN1 Human)
UseTagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-BP
Plasmid#60512PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.HI.eGFP-Cre.WPRE.SV40
Plasmid#105545PurposeAAV expression of EGFP-Cre from CMV promoterDepositorHas ServiceAAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP-Cre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterHIAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/HA-SUMO3 (Sentrin 2)
Plasmid#17361PurposeMammalian expression of human SUMO3 with HA tagDepositorInsertSUMO3 (SUMO3 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS TIR-1 sfGFP
Plasmid#154463PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pToneDest
Plasmid#67691PurposeattR4-attR3 Destination cassette in Tol1 backboneDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
CD59-bio-His
Plasmid#53154PurposeExpresses full-length CD59 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCD59 (CD59 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Beta-arrestin2 GFP WT
Plasmid#35411DepositorInsertβarrestin2 (Arrb2 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only
