20,460 results
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pCdrA-gfpC
Plasmid#111614PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(Mut3)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-FLT3
Plasmid#23895DepositorInsertFLT3 (FLT3 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS Exon Vector
Plasmid#69901PurposeExpression plasmid for expressing circular RNAs of a desired sequence in mammalian cellsDepositorInsertZKSCAN1 (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACgp67B-Her2
Plasmid#10794DepositorInsertErbB2 ScFv (ERBB2 Human)
UseTags6HisExpressionInsectMutationPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pBAD33-mf-lon
Plasmid#21867DepositorInsertLon (Mfl404 Mesoplasma florum)
UseTagsExpressionBacterialMutationall TGA codons changed to TGG codonsPromoterAvailable sinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1-HA
Plasmid#24399DepositorInsertBeclin1 (BECN1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA-mDyn3 pcDNA3
Plasmid#36265DepositorInsertDynamin 3 (Dnm3 Mouse)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT wild type
Plasmid#13639DepositorUseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
HA-Beclin 1
Plasmid#86748PurposeN-terminal HA-tagged protein expression in mammalian cellsDepositorInserthuman Beclin 1 (BECN1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.3 Thy1.1
Plasmid#14749DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NE1h
Plasmid#123309PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE sensor GRAB_NE1h
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFR3.v857.PDGFR
Plasmid#178329PurposeFluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857DepositorHas ServiceAAV1InsertpAAV hSyn iGluSnFR3 v857.PDGFR
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and PDGFR TM DomainExpressionMammalianMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEJS614_pTetR-P2A-BFPnls/sgNS
Plasmid#108650PurposeNon-specific Spy sgRNA under U6 promoterDepositorInsertsNon-specific sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterU6 and hPGKAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl_AXP
Plasmid#84608PurposeIntroduces DSB at AXP locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXPG
Plasmid#71248Purposeimproved luciferase reporter gene plasmidDepositorTypeEmpty backboneUseLuciferaseTagsluciferaseExpressionMammalianMutationPromoternoneAvailable sinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET_RTX-6xHis
Plasmid#145029PurposepET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tagDepositorArticleInsertRTX
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1651-sgRNA(F+E)-sgGal4
Plasmid#100549PurposesgGal4-expressing vector modified from Addgene plasmid #51024DepositorInsertsgGal4
UseCRISPRTagssgRNAExpressionMammalianMutationPromoterU6Available sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Snail_pGL2
Plasmid#31694DepositorInsertSnail (SNAI1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141A
Plasmid#69290PurposeGateway entry vector; single gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2
Plasmid#87694PurposeExpress ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cellsDepositorInsertERT2-PhoCl-Cre-PhoCl-ERT2
UseTagsExpressionMammalianMutationPromoterChicken beta actinAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-EYFP-WPRE
Plasmid#104111PurposeAAV-mediated expression of EYFP under the TRE promoter.DepositorInsertEYFP
UseAAVTagsExpressionMutationPromoterTREAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB405
Plasmid#74799PurposeGateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
POLD1_pLX307
Plasmid#98358PurposeLentiviral expression of POLD1DepositorInsertPOLD1 (POLD1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-dL5
Plasmid#73214PurposeBacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)DepositorInsertEGFR (EGFR Human, Schistosoma japonicum)
UseTags10XHis-GST-HRVExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZJOM65
Plasmid#133654PurposeIntegration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.DepositorInsertp1k-VPH1-mCherry (VPH1 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH (AAV9)
Viral Prep#100050-AAV9PurposeReady-to-use AAV9 particles produced from pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH (#100050). In addition to the viral particles, you will also receive purified pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH plasmid DNA. CaMKIIa-driven, ChETA(E123T/H134R)-eYFP expression for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsEYFPAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMJ117
Plasmid#85997PurposeOne-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3DepositorInsertsEGFP-NT2_cr3
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermodified human U6Available sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-Rac1
Plasmid#83950PurposeRac1 FLIM donor for dynamic imaging of GTPase activityDepositorInsertras-related C3 botulinum toxin substrate 1 (RAC1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV BAF170-Flag
Plasmid#19142DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (SMARCC2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry
Plasmid#85430Purpose[Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with constitutive mCherry expressionDepositorInserthuman IL-10 T2A PD-L1
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-nlsCRE
Plasmid#12265DepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SENP8
Plasmid#18066DepositorInsertSENP8 (SENP8 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGCS-N3(3xFLAG)
Plasmid#85720PurposeGateway destination vectorDepositorTypeEmpty backboneUseMammalian/avian/xenopus/zebrafishTags3xFLAGExpressionMutationPromoterAvailable sinceFeb. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-D1ER
Plasmid#36325DepositorInsertD1ER second generation cameleon (calcium sensor) targeted to ER
UseTagsKDELExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pBUN421
Plasmid#62204PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterTaU3 and UbiAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-PA-Rac1-T17N
Plasmid#22029PurposeExpression of photoactivatable Rac1 (dominant-negative mutant).DepositorInsertPA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
UseTags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-hNOS2
Plasmid#110800Purposetet-inducible NOS2 expressionDepositorInsertNOS2 (NOS2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLSSmKate1-N1
Plasmid#31866DepositorInsertLSS-mKate1
UseTagsExpressionMammalianMutationK69Y/P131T/S148G/M167E/T183S/M196V compared to mK…PromoterCMVAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAnc80L65
Plasmid#68837PurposeAdeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for detailsDepositorInsertAncestral AAV Capsid Anc80L65
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterRep2Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET his-eGFP
Plasmid#113551PurposeFor bacterial expression and purification of eGFPDepositorInserthis-eGFP
UseTagsExpressionBacterialMutationA206KPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp Phy-mCherry-CAAX
Plasmid#50839PurposeOptogenetic PI3K activation system; membrane componentDepositorInsertPhyB 1-908
UseLentiviral and Synthetic BiologyTagsmCherryExpressionMutationPromoterSFFVAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
ADRA2A-Tango
Plasmid#66216PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertADRA2A (ADRA2A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSET-dCas9
Plasmid#110183PurposeExpression of the dCas9 gene in StreptomycesDepositorInsertdCas9
UseCRISPR; ITagsExpressionBacterialMutationD10A, H840APromoterermE*pAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET28-SBP-TEV
Plasmid#36943PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTags6xHistag-SBP-TEVExpressionBacterialMutationPromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pSK+KanaRpsL
Plasmid#20871DepositorInsertKanamycinResistance-RpsL
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only
