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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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181,361 results

Showing: 1851 - 1900 of 181361 results

  4. pSRG100 (germline optimized 3xGCN4 for C. elegans)

    Plasmid
    #246457
    Purpose
    Encodes germline optimized 3xGCN4 for C. elegans. Useful to make CRISPR/Cas9 repair templates for 3xGCN4 integration in a gene of interest. For SunTag live-imaging of translation or protein labelling.
    Depositor
    Suzan Ruijtenberg
    Insert
    3xGCN4 (GLO)
    Expression
    Worm
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    246457-plasmid-map-sequence-id-496369

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  5. pSRG101 (KasI::24xGCN4::T2A::AvrII)

    Plasmid
    #246458
    Purpose
    24xGCN4::T2A with flanking restriction sites (KasI and AvrII). Useful for cloning SunTag reporter plasmids for expression in C. elegans.
    Depositor
    Suzan Ruijtenberg
    Insert
    24xGCN4::T2A
    Expression
    Bacterial
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    246458-plasmid-map-sequence-id-496351

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  7. VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-srIκBα

    Plasmid
    #242411
    Purpose
    Self-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (E3, NSs, L*) and inflammatory signaling inhibitor, super repressor inhibitor of κBα (srIκBα).
    Depositor
    Ewan St. John Smith
    Article
    Lim et al bioRxiv 2024.09.24.614636
    Inserts
    mScarlet3
    IRES-E3-NSs-L*-EGFP
    IRES-srIκBα
    Use
    Mouse Targeting; Self-amplifying rna
    Expression
    Mammalian
    Mutation
    serines 32 and 36 to alanines
    Available Since
    Sept. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    242411-plasmid-map-sequence-id-487192

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  8. CMV/CRAF-SmBiT/BlastR Vector

    Plasmid
    #238811
    Purpose
    Express CRAF-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    CRAF (RAF1 Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238811-plasmid-map-sequence-id-479920

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  9. CMV/BRAF-SmBiT/BlastR Vector

    Plasmid
    #238813
    Purpose
    Express BRAF-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    BRAF (BRAF Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238813-plasmid-map-sequence-id-479921

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  10. CMV/BRAF-LgBiT/HygR Vector

    Plasmid
    #238814
    Purpose
    Express BRAF-LgBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    BRAF (BRAF Human)
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238814-plasmid-map-sequence-id-479837

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  11. SAGA005

    Plasmid
    #240754
    Purpose
    Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.
    Depositor
    Morten Norholm
    Article
    Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:
    Insert
    SAGA p15A J23100 BCD_medium - gfp - tetAp15A- CmTrunc
    Use
    Synthetic Biology
    Mutation
    Mutation on translation initiation region of tetA
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    240754-plasmid-map-sequence-id-483915

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  24. pET3d-SF-DCTN1-TS

    Plasmid
    #169375
    Purpose
    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Bernd Bukau
    Article
    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi
    Insert
    dynactin subunit 1 isoform 1 (DCTN1 Human)
    Tags
    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    Point mutations: A139T, I304T, Q322R, T516A, E577…
    Available Since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169375-plasmid-map-sequence-id-333771

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  36. pAAV.Syn.GCaMP6f.WPRE.SV40 (AAV1)

    Viral Prep
    #100837-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.GCaMP6f.WPRE.SV40 (#100837). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Available Since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only

  42. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV9)

    Viral Prep
    #162379-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  44. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid
    #198468
    Purpose
    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.
    Depositor
    Viviana Risca
    Article
    Soroczynski et al bioRxiv 2024.07.11.602973
    Insert
    10His-pG-Tn5 (E54K, L372P)
    Tags
    10His-pG
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    Consensus φ10 T7 Promoter
    Available Since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198468-plasmid-map-sequence-id-390256

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  46. 3xFlag-Rab34

    Plasmid
    #232458
    Purpose
    Expresses 3xFlag-tagged human Rab34 in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    Rab34 (RAB34 Human)
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232458-plasmid-map-sequence-id-463631

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  50. pAAV-EF1a-Cre (AAV Retrograde)

    Viral Prep
    #55636-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-Cre (#55636). In addition to the viral particles, you will also receive purified pAAV-EF1a-Cre plasmid DNA. EF1a-driven expression of Cre recombinase. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Available Since
    Nov. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1851 - 1900 of 181361 results