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166,787 results

Showing: 1851 - 1900 of 166787 results

  2. pGP-AAV-syn-jGCaMP8s-WPRE (AAV1)

    Viral Prep
    #162374-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Tags
    None
    Available Since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  9. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV9)

    Viral Prep
    #162379-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Tags
    None
    Available Since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  10. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid
    #85452
    Purpose
    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.
    Depositor
    Aviv Regev
    Article
    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do
    Insert
    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    Use
    CRISPR and Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    85452-plasmid-map-sequence-id-173969

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  15. Lenti-multi-Guide

    Plasmid
    #85401
    Purpose
    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    85401-plasmid-map-sequence-id-322305

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  21. BPK1520

    Plasmid
    #65777
    Purpose
    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SpCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65777-plasmid-map-sequence-id-316938

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  23. pCR2.1-InC

    Plasmid
    #83959
    Purpose
    Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.
    Depositor
    Kai-Shun Chen
    Insert
    Internal Control sequence
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83959-plasmid-map-sequence-id-160468

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  26. CIDAR MoClo Parts Kit

    Plasmid Kit
    #1000000059
    Purpose
    CIDAR Modular Cloning (MoClo) kit uses modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in E. coli
    Depositor
    Douglas Densmore
    Available Since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  27. pAAV-hSyn-hChR2(H134R)-EYFP (AAV Retrograde)

    Viral Prep
    #26973-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    EYFP
    Available Since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only

  30. AAV.TBG.PI.Cre.rBG

    Plasmid
    #107787
    Purpose
    AAV vector expressing Cre from TBG promoter
    Depositor
    James M. Wilson
    Has Service
    AAV8
    Insert
    Cre
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TBG
    Available Since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    107787-plasmid-map-sequence-id-259375

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  31. p6XHis-T7(P266L)

    Plasmid
    #174866
    Purpose
    Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.
    Depositor
    Anna Pyle
    Article
    Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.
    Insert
    T7 RNA polymerase
    Use
    Tags
    6XHis-tag
    Expression
    Bacterial
    Mutation
    P266L
    Promoter
    lac promoter
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174866-plasmid-map-sequence-id-419578

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  35. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV8)

    Viral Prep
    #105553-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    None
    Available Since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only

  41. pMitoTimer

    Plasmid
    #52659
    Purpose
    Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo
    Depositor
    Zhen Yan
    Article
    Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1
    Insert
    DsRed1-E5
    Use
    Dsred
    Tags
    human cytochrome c oxidase subunit VIII mitochond…
    Expression
    Mutation
    Promoter
    CMV
    Available Since
    April 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    52659-plasmid-map-sequence-id-430007

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  46. pGP-AAV-syn-jGCaMP8s-WPRE (AAV9)

    Viral Prep
    #162374-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Tags
    None
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only

  50. pAAV-Ef1a-mCherry

    Plasmid
    #114470
    Purpose
    mCherry Control
    Depositor
    Karl Deisseroth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    mCherry
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Ef1a
    Available Since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114470-plasmid-map-sequence-id-220933

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Showing: 1851 - 1900 of 166787 results