We narrowed to 15,942 results for: LEA

1. IK349

   Plasmid

   #233655

   Purpose

   Dox-inducible red light-inducible Flp-C; Flp-397C-PhyA

   Depositor

   Wilson Wong

   Article

   Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

   Insert

   Flp-397C-PhyA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   TRE3G

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSP1673

   Plasmid

   #65769

   Purpose

   Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-GFP11-pDEST-N

   Plasmid

   #118366

   Purpose

   Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   GFP11-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. ND4.2-Left TALE-G1397-N-dead-mCherry

   Plasmid

   #179683

   Purpose

   Expression of base editor in mammalian cells and for cell sorting

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1347A

   Promoter

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AD59_pEP.DonorCLYBL.TS

   Plasmid

   #199227

   Purpose

   Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   Expression unit for PuroR.T2A.EGFP selectable and reporter markers
   

   Use

   Gene targeting donor plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1 gene promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcKv10.1

   Plasmid

   #85703

   Purpose

   mammalian expression Kv10.1

   Depositor

   Luis Pardo

   Article

   Chen et al PLoS One. 2011 May 3;6(5):e19257. doi: 1

   Insert

   KCNH1 (KCNH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRevTRE MKL1

   Plasmid

   #19846

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

8. AAV sgRNA Expression Plasmid

   Plasmid

   #174540

   Purpose

   Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

   Depositor

   Ormond MacDougald

   Article

   Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

   Type

   Empty backbone

   Use

   AAV

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ220-Ultra

   Plasmid

   #176889

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

   Insert

   AsCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1 puro_shTBK1_3185

    Plasmid

    #167294

    Purpose

    Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    shRNA TBK1 (TBK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19/NLS-mChe

    Plasmid

    #183162

    Purpose

    Construct for transient expression of cell nucleus-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    NLS-mCherry
    

    Use

    Tags

    16 aa nuclear localization signal and 16 aa nucl…

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACS131

    Plasmid

    #204596

    Purpose

    Histone H3.1K27M-V5

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    histone H3.1 K27M (H3C1 Human)
    

    Use

    Lentiviral

    Tags

    human histone H3.1-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

    Plasmid

    #87694

    Purpose

    Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    ERT2-PhoCl-Cre-PhoCl-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. dTomato_LD 0_CS M_tTA-BFP

    Plasmid

    #58875

    Purpose

    MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-nls-sfGFP

    Plasmid

    #132973

    Purpose

    full-length of nuclear localized sfGFP with stop codon in pmE Gateway vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    nuclear localized super folder GFP
    

    Use

    Gateway vector

    Tags

    6x myc and SV40 NLS

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. gRNA-trp-HYB

    Plasmid

    #64331

    Purpose

    trp1 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of trp1 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pH-mono-MAD7

    Plasmid

    #170134

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al J Genet Genomics. 2021 May 3. pii: S1673

    Insert

    MAD7(ErCas12a)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    maize Ubiquitin-1, switchgrass Ubiquitin-1

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-CD9-(TEV)-tTA

    Plasmid

    #162596

    Purpose

    human CD9-TEV cleavage site-tTA

    Depositor

    Masaharu Somiya

    Article

    Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

    Insert

    human CD9 (CD9 Human)
    

    Use

    Tags

    TEV protease cleavage site and tTA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAcCas12nHS_ABE_D3_empty

    Plasmid

    #203809

    Purpose

    Plasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    ABE_D3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV4-NIK-HA

    Plasmid

    #27554

    Depositor

    Shao-Cong Sun

    Article

    Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

    Insert

    NIK (MAP3K14 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    NIK starts at amino acid 3; C52R and R139H mutati…

    Promoter

    CMV

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

21. eGFP-MYO5B

    Plasmid

    #239265

    Purpose

    Expression of N terminal GFP tagged Myo5B

    Depositor

    David Pellman

    Article

    Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

    Insert

    MYO5B (MYO5B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET41a iGluu

    Plasmid

    #119834

    Purpose

    Production of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluu
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    S72T

    Promoter

    T7

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry

    Plasmid

    #182390

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-mNeongreen-His
    OmpA-mCherry
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFP-N1.ARRDC1

    Plasmid

    #38320

    Depositor

    Quan Lu

    Article

    Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    ARRDC1 (ARRDC1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p2T-CMV-eA3A-BE5-NG-BlastR

    Plasmid

    #152998

    Purpose

    High purity C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-C1-KSENB-Nup133

    Plasmid

    #87340

    Purpose

    To express human Nup133 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of Nup133 AA1-7

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEJS613_pTetR-P2A-BFPnls/sgTelo

    Plasmid

    #108649

    Purpose

    Telomere-targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Telomere-targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLaNGo11-7

    Plasmid

    #126055

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAIP Nup62Fv2GFP

    Plasmid

    #139684

    Purpose

    Nup62 dimerization construct fused to 2 copies of GFP under an SFFV promoter

    Depositor

    Edward Campbell

    Article

    Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

    Insert

    Nup62 (NUP62 Human)
    

    Use

    Lentiviral

    Tags

    2 copies of GFP and Dimerization domain FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. UBXD1-GFP

    Plasmid

    #86464

    Purpose

    Expression of human UBXD1 with C-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    UBXD1 (UBXN6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-nuc-Nb37

    Plasmid

    #205515

    Purpose

    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb37
    

    Use

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pNOC-CRISPR-GFP

    Plasmid

    #100009

    Purpose

    Expresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneome

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-GFP

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a_Pro-IL-1α

    Plasmid

    #214341

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL1A (IL1A Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFYF1548 EMX1

    Plasmid

    #47508

    Purpose

    human gRNA expression vector targeting EMX1

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EMX1 (EMX1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. hJAM-A pcDNA3.1

    Plasmid

    #70073

    Purpose

    Expresses human junctional adhesion molecule-A in mammalian cells

    Depositor

    Terence Dermody

    Article

    Danthi et al J Virol. 2006 Feb;80(3):1261-70.

    Insert

    junctional adhesion molecule-A (F11R Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. His-AgdNK

    Plasmid

    #203226

    Purpose

    For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
    

    Use

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

    Plasmid

    #60815

    Purpose

    dCas9 with single cysteine residue (M1C) for site-specific labelling

    Depositor

    Jennifer Doudna

    Article

    O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

    Insert

    dCas9 M1C D10A C80S H840A C574S
    

    Use

    Tags

    His10 and MBP

    Expression

    Bacterial

    Mutation

    M1C D10A C80S H840A C574S

    Promoter

    Available Since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mCer-TG2-YFP

    Plasmid

    #85471

    Purpose

    TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

    Depositor

    Jeffrey Keillor, Ray Truant

    Article

    Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

    Insert

    hTG2 (TGM2 Human)
    

    Use

    Tags

    EYFP and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pPBAD-yhjH

    Plasmid

    #111613

    Purpose

    Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

    Depositor

    Tim Tolker-Nielsen

    Article

    Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

    Insert

    yhjH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A52V

    Promoter

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA-flag-mKdm4d-polyA

    Plasmid

    #61553

    Purpose

    For mRNA synthesis or expression in mammalian cells

    Depositor

    Yi Zhang

    Article

    Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

    Insert

    Kdm4d (Kdm4d Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. SQT1665

    Plasmid

    #78744

    Purpose

    Human expression plasmid for LbCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEGFP-AID

    Plasmid

    #61926

    Purpose

    Expression of human AID tagged with EGFP

    Depositor

    Jon Houseley

    Article

    Lambert et al PLoS One. 2013 Dec 4;8(12):e82110. doi:

    Insert

    AICDA (AICDA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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44. pNOC-ARS-CRISPR-v2

    Plasmid

    #99863

    Purpose

    Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Tags

    Expression

    Mutation

    Promoter

    Ribi

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pFloat.cspA_v1

    Plasmid

    #133071

    Purpose

    Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

    Depositor

    David Sabatini, Kacper Rogala

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    cspA

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. SQT1659

    Plasmid

    #78743

    Purpose

    Human expression plasmid for AsCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. pHJ423

    Plasmid

    #20681

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Thr 567 to Asp

    Promoter

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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48. R26-EGFP HMEJ donor vector

    Plasmid

    #137927

    Purpose

    ROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-EGFP-bpA with homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pcDNA6A-EGFR ECD (1-644)

    Plasmid

    #42666

    Depositor

    Mien-Chie Hung

    Article

    Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

    Insert

    EGFR ECD (1-644) (EGFR Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

    Plasmid

    #132962

    Purpose

    Firefly-luciferase single-strand annealing assay cloning backbone

    Depositor

    Gang Bao

    Article

    Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

    Insert

    Firefly Luciferase
    

    Use

    CRISPR and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Stop sequence between luciferase gene

    Promoter

    CMV

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge