Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

Applied Filters

20+ requests Clear filter

20,802 results

Showing: 4901 - 4950 of 20802 results

  3. pRK5-myc-RhoA-wt

    Plasmid
    #12962
    Depositor
    Gary Bokoch
    Insert
    RhoA (RHOA Human)
    Tags
    Myc
    Expression
    Mammalian
    Available Since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV.hSyn.eGFP.WPRE.bGH

    Plasmid
    #105539
    Purpose
    AAV expression of EGFP from hSyn promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105539-plasmid-map-sequence-id-457689

    Enlarge

  7. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (AAV Retrograde)

    Viral Prep
    #162379-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP8f-WPRE (#162379). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8f-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of the ultrafast calcium sensor, GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  9. pAAV.CAG.GCaMP6f.WPRE.SV40 (AAV1)

    Viral Prep
    #100836-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.GCaMP6f.WPRE.SV40 (#100836). In addition to the viral particles, you will also receive purified pAAV.CAG.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    CAG
    Available Since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. pBUE411-GGB

    Plasmid
    #198233
    Purpose
    Wheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411
    Depositor
    Andrea Gallavotti
    Article
    Chen et al bioRxiv 2022.09.02.506370
    Inserts
    GRF4-GIF1 chimera cassette
    pPLTP::BBM cassette
    Use
    CRISPR; Plant binary vector
    Expression
    Plant
    Promoter
    Ubiquitin and maize PLTP
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    198233-plasmid-map-sequence-id-495715

    Enlarge

  19. pCW57.1-FOXM1c

    Plasmid
    #68810
    Purpose
    Doxycycline inducible lentiviral vector for FOXM1c expression in mammalian cells
    Depositor
    Adam Karpf
    Article
    Barger et al Oncotarget. 2015 Jul 16.
    Insert
    FOXM1c (FOXM1 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    TRE promoter, Tet ON
    Available Since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    68810-plasmid-map-sequence-id-232051

    Enlarge

  31. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Article
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    U1a and U6
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112139-plasmid-map-sequence-id-217823

    Enlarge

  35. mEGFP-pBAD

    Plasmid
    #54622
    Purpose
    Localization: Protein Expression Vector , Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    6xHis and mEGFP
    Expression
    Bacterial
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54622-plasmid-map-sequence-id-257491

    Enlarge

  37. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

    Enlarge

  41. pAAV.GFAP.Cre.WPRE.hGH

    Plasmid
    #105550
    Purpose
    AAV expression of Cre from GFAP promoter
    Depositor
    James M. Wilson
    Has Service
    AAV PHP.eB and AAV5
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    GFAP
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105550-plasmid-map-sequence-id-202448

    Enlarge

  42. FokI-dCas9

    Plasmid
    #52970
    Purpose
    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells
    Depositor
    David Liu
    Article
    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    Fok1 fused to dCas9
    Use
    CRISPR
    Tags
    Fok1 nuclease (homodimeric wildtype) and NLS
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9
    Promoter
    CMV
    Available Since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    52970-plasmid-map-sequence-id-82408

    Enlarge

  43. pAAV-CAG-FLEX-EGFP

    Plasmid
    #59331
    Purpose
    Expresses EGFP
    Depositor
    Ian Wickersham
    Has Service
    AAV1
    Insert
    EGFP
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    59331-plasmid-map-sequence-id-468735

    Enlarge

  45. pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV Retrograde)

    Viral Prep
    #26975-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CamKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII
    Tags
    mCherry
    Available Since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only

  49. pJR101

    Plasmid
    #187241
    Purpose
    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    Use
    Lentiviral
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    187241-plasmid-map-sequence-id-374769

    Enlarge

Showing: 4901 - 4950 of 20802 results