20,415 results
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pET28a-uvsX-H6
Plasmid#163913PurposeExpresses uvsX for bacterial expression and affinity purificationDepositorInsertuvsX
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL-MS2-12X
Plasmid#27119DepositorInsertMS2 binding sites (12)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV Puro DEST p38KTRmCerulean3
Plasmid#59155PurposeLentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)DepositorInsertp38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
CAG-GFP
Plasmid#16664DepositorInserteGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
mKeima-Red-N1
Plasmid#54597PurposeLocalization: N1 Cloning Vector, Excitation: 440, Emission: 620DepositorTypeEmpty backboneUseTagsmKeima-RedExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(4)P Probe
Plasmid#211509PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteriaDepositorInsertSidC (P4C)
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion T2A-H2B-mCherry
Plasmid#113089PurposeGeneral donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donorDepositorInsertT2A-H2B-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRDA_479
Plasmid#179099PurposeBE Cas expressionDepositorInsertABE8e (SpG)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
ΔNp63gamma-FLAG
Plasmid#27012DepositorInsertΔNp63gamma (TP63 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pXR004: CasRx pre-gRNA cloning backbone
Plasmid#109054PurposehU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-NEMOc
Plasmid#189933PurposeMammalian expression of calcium sensor with ultra-high dynamics and sensitivityDepositorInsertNEMOc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHes1-GFPd2
Plasmid#14808DepositorInsertHes1 promoter
UseTagsGFPd2ExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAL119-TK
Plasmid#21911DepositorInsertHSV Type 1 thymidine kinase
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTP434
Plasmid#104167PurposeBacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labelingDepositorInsertAnti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
UseTags14x Histidine tag and NEDD8 from Brachypodium dis…ExpressionBacterialMutation3x Cysteines located at bps 493-495, 520-522, and…PromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
hACE2
Plasmid#1786PurposeMammalian expression of human ACE2DepositorUseTagsC9 (GTETSQVAPA)ExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTRIPZ (M)-H2A-HT
Plasmid#82517PurposeLentivirus vector. Expresses H2A-Halotag fusion proteins in mammalian cells.DepositorInsertHistone H2A
UseLentiviralTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-Cas9-T2A-EGFP-ires-puro
Plasmid#78311PurposeExpresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.DepositorInsertWT SpCas9-T2A-EGFP-ires-puromycin resistance
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianMutationPromoterCAGAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCS2 Flag-JunB
Plasmid#29687DepositorInsertjun B oncogene (JUNB Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-F-del Casp9.IRES.GFP
Plasmid#15567DepositorInsertFKBP12(V36)-p30Caspase9 (FKBP1A Human)
UseRetroviralTagsFKBP12 and HA epitopeExpressionMammalianMutationFKBP12 F36 to V Truncated Caspase9 (135-416; lac…PromoterAvailable sinceAug. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
5HRE/GFP
Plasmid#46926Purposehypoxia-responsive enhanced green fluorescent protein (EGFP)-based systemDepositorInsert5X HRE of VEGF (VEGFA Human)
UseTagsd2EGFPExpressionMammalianMutationfive copies of a 35-bp fragment from the hypoxia-…PromoterEF-1α promoterAvailable sinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-H2B-GCaMP6s
Plasmid#59530PurposeExpressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsUseTransposable elementTagsExpressionMutationPromoterAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLEX-MCS-ASC-GFP
Plasmid#73957PurposeExpress human ASC with a C-terminal EGFP tag in mammalian cellsDepositorInsertPYD and CARD domain containing, PYCARD (PYCARD Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
SP6-VEE-GFP
Plasmid#58976Purposeexpression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA repliconDepositorInsertEGFP
UseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoter26S subgenomic promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.HI.eGFP-Cre.WPRE.SV40
Plasmid#105545PurposeAAV expression of EGFP-Cre from CMV promoterDepositorHas ServiceAAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP-Cre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterHIAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV OMM-FKBP-V5-sTurboID (N)
Plasmid#153006PurposeExpresses split-TurboID N-terminal fragment targeted to outer mitochondrial membraneDepositorInsertOMM (Tom20)-sTurboID (N) (L73)
UseLentiviralTagsFKBP and V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAP4K4
Plasmid#23486DepositorInsertMAP4K4 (MAP4K4 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP-BAZ1B
Plasmid#65372PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertBAZ1B (BAZ1B Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cdc42-2G
Plasmid#68813PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)DepositorInsertCdc42 activation reporter
UseLentiviralTagsHis and MycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1
Plasmid#187120Purposemammalian cell expression of FLAG-HUWE1DepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag ERbeta
Plasmid#35562DepositorInsertEstrogen Receptor beta (ESR2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
biGBac Kit
Plasmid Kit#1000000088PurposeGeneration of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.DepositorAvailable sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mito-mEosEM
Plasmid#132706Purposeapplication in Correlative light-electron microscopyDepositorInsertmitochondria targeting sequence
UseTagsmEosEMExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
RAR-TRE-mStrawberry reporter
Plasmid#158683PurposeThis plasmid is a RAR pathway reporter. It has a Retinoic Acid Receptor Response Element promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-TOMM20-N-10
Plasmid#54282PurposeLocalization: Mitochondrial Membrane, Excitation: 487, Emission: 509DepositorInsertTOMM20 (TOMM20 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
dRED
Plasmid#139836PurposeExpresses dRNase H1-EGFP-dCas9 fusion protein in human cellsDepositorInsertdRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
UseTagsExpressionMammalianMutationRNase H1 protein contains D210N mutation. dCas9 …Promotertet-inducible CMVAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXL002-ishRNA-beta-catenin-1
Plasmid#36297DepositorInsertshRNA of beta-catenin-1 (CTNNB1 Human)
UseLentiviral and RNAiTagsIRES-RFP-PuroExpressionMammalianMutationPromoterTetO-H1Available sinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam p53 R248W
Plasmid#16437DepositorInsertp53 R248W (TP53 Human)
UseTagsExpressionMammalianMutationR248WPromoterAvailable sinceAug. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE
Plasmid#85452PurposeLentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.DepositorInsertU6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationPromoterAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC32-HPB
Plasmid#32078DepositorTypeEmpty backboneUseGateway destination binary vectorTagsHA-Prescission-BiotinExpressionMutationPromoter35S promoterAvailable sinceDec. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-MTM1
Plasmid#51614PurposeRecruitable PtdIns3P/PtdIns(3,5)P2 phosphataseDepositorInsertsUseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
HTR2C-Tango
Plasmid#66411PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertHTR2C (HTR2C Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] (AAV8)
Viral Prep#130909-AAV8PurposeReady-to-use AAV8 particles produced from AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] (#130909). In addition to the viral particles, you will also receive purified AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] plasmid DNA. CAG-driven, Flp-dependent expression of ChrimsonR-tdTomato. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Flp-dependent)Available sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-mScarlet-Gephyrin.FingR-IL2RGTC
Plasmid#125695PurposeGlobal labeling of inhibitory synapses (red fluorescence)DepositorInsertmScarlet-Gephyrin.FingR-IL2RGTC
UseAAVTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-EGFP-SHP2WT
Plasmid#12283DepositorInsertSHP-2WT (PTPN11 Human)
UseTagsMyc (not canonical: KLISEEDL)ExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV MaCPNS1)
Viral Prep#37825-MaCPNS1PurposeReady-to-use AAV MaCPNS1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV were produced with the MaCPNS1 serotype, which permits efficient transduction of the PNS in rodents and the CNS and PNS (including ENS) in marmosets and rhesus macaques. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCutamp
Plasmid#140632PurposePlasmid-curing in Escherichia coli by targeting the AmpR promoterDepositorInsertSpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPCX cyto Grx1-roGFP2
Plasmid#64975PurposeMammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)DepositorInsertGlutaredoxin-1 (GLRX Human)
UseRetroviralTagsroGFP2ExpressionMammalianMutationPromoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-hGSDMD
Plasmid#111559PurposeExpress human GSDMD in E.coliDepositorInsertGasdermin D (GSDMD Human)
UseTagsHis, SUMOExpressionBacterialMutationPromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only
