76,413 results
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TFORF3311
Plasmid#144787PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCREB3 (CREB3 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLCKO
Plasmid#73311PurposeLentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10871
Plasmid#183962PurposeU6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPRDepositorInsertsdCas12a(wildtype)
poly-crRNA array targeting Sox2-Klf4-Oct4
UseCRISPRTagsHAExpressionMammalianMutationD832APromoterCAG and U6Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVdel-dimericTomato-WASp(CRIB)
Plasmid#191450PurposeVisualizing endogenous & active Cdc42DepositorInsertWASp
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV.PARP1#2
Plasmid#14545DepositorInsertPARP1 shRNA (PARP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HshnRNPR_AA
Plasmid#148235PurposeMammalian Expression of HshnRNPRDepositorInsertHshnRNPR (HNRNPR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
NanoBRET Assay Vector, HaloTag-APC
Plasmid#237145PurposeExpress HaloTag(R)-APC Fusion Protein in Mammalian Cells under a CMV promoterDepositorUseTagsHaloTag (R)ExpressionMammalianMutationNonePromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9
Plasmid#223321PurposeIt contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.DepositorInsertsUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCBhAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Pyl-TAG
Plasmid#228784PurposePyl aaRS/tRNA system for mammalian TAG suppressionDepositorInsertsPylRS
PytR
UseBaculoviralTagsExpressionMammalianMutationTAG suppressorPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-3JB1F+6
Plasmid#190835PurposeFor mammalian expression of 3JB1F+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert3JB1F_T1+6bp (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.3_CoV2_501V2
Plasmid#170449PurposeExpress SARS-CoV-2 501V2 (South Africa strain / beta strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 501V2 pseudotyped virus production.DepositorInsertSARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, 501V2 (B.1.351))
UseTagsNAExpressionMammalianMutationL18F, D80A, D215G, R246I, K417N, E484K, N501Y, D6…PromoterCMVAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-RIPK3
Plasmid#78804PurposeTo overexpress RIPK3 in Mammalian CellsDepositorInsertRIPK3 (RIPK3 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/20]
Plasmid#206744PurposeMammalian Expression of GFP scFV. Derived from hybridoma N86/20 scFv.DepositorInsertGFP (Aequorea victoria) recombinant scFV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN5-ires-blast
Plasmid#167822PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN5
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_G3BP1
Plasmid#127106DepositorInsertG3BP1 (G3BP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Siah2 Ring Mutant
Plasmid#122042Purposeexpress Siah2 RMDepositorInsertSiah2 RM (Siah2 Mouse)
UseTagsFlagExpressionMammalianMutationRing mutant deletion H98, L99A, V100G, C101APromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMarsL274G
Plasmid#63177PurposeThis plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.DepositorInsertMarsL274G (Mars1 Mouse)
UseTagsExpressionMammalianMutationL274GPromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP33
Plasmid#22601DepositorInsertUSP33 (USP33 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-NET-myc
Plasmid#107458PurposeMammalian expression plasmid for codon optimized hNET with internal MYC tag inserted in ECL2DepositorInsertNorepinephrine Transporter (NET) (SLC6A2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
SNAP-Sec61B
Plasmid#141152PurposeFluorescent labeling of ER membrane in mammalian cellsDepositorInsertSec61 beta (SEC61B Human)
UseTagsSNAP tagExpressionMammalianMutationL79M on Sec61BPromoterCMVAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-SEP-NRI
Plasmid#23999DepositorInsertNR1 (Grin1 Rat)
UseTagsNR1 signal sequence (aa1-20) and SEP (supereclipt…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
lenti-NLS-mTagBFP2
Plasmid#216128PurposeLentiviral vector for expressing mTagBFP2 localizing to the nucleusDepositorInsertEF1a-NuclearLocalizationSignal-mTagBFP2-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK6 wt
Plasmid#80882Purposemammalian expression of ALK6 WTDepositorInsertALK6 (Bmpr1b Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAdTrack-PPARdelta
Plasmid#16529DepositorInsertPPAR delta (PPARD Human)
UseAdenoviralTags2x HA (YDVPDYAS)ExpressionMammalianMutationPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-XBP1u-FLuc
Plasmid#31239DepositorInsertFLAG-XBP1u-FLuc (XBP1 Human)
UseTagsFlag and FlucExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
HA-AR-LBD
Plasmid#171239PurposeMammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)DepositorInsert1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
plk1 T210D
Plasmid#132964PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorInsertpolo-like kinase 1 (PLK1 Human)
UseTagsExpressionMammalianMutationT210DPromoterAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag16-MUC4
Plasmid#205198PurposeThis vector encodes of the synCAM (MUC4) and GFP nanobody LAG16DepositorInsertsynCAM MUC4 and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-iCre-P2A-tdTomato-T2A-Puro
Plasmid#72255PurposeExpress iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoterDepositorInsertiCre-P2A-tdTomato-T2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterCB and chicken beta-actin + CMV enhancerAvailable sinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PIP5K1B
Plasmid#202722Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertEGFP:PIP5K1B (PIP5K1B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR
Plasmid#81253PurposeLentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selectionDepositorInsertTetR and LacI
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
AIO-mCherry
Plasmid#74120PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE
Plasmid#20945Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsExpressionMammalianMutationH134R mutation in ChR2PromoterhSynAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pHCMV_OROV_GP
Plasmid#207297PurposeMammalian expression of Oropouche virus GP proteinDepositorInsertOROV_GP (M Oropouche virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Pur-mEGFP
Plasmid#186329PurposeFluorescent reporter systemDepositorInsertmEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOBCol2.3-GFPemd
Plasmid#110210PurposeLabels cells in the late osteoblast lineage.DepositorInserteGFPemerald
UseTagsNoneExpressionMammalianMutationPromoter2.3 kb rat type 1 collagen + first intronAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-SpRY-P2A-GFP
Plasmid#159981PurposePrime editing in mammalian cellsDepositorInsertPE2-SpRY-P2A-GFP
UseTagsBpSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
Plasmid#42337PurposeDual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.DepositorArticleInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pcsII-LMP7-flag
Plasmid#86773PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertimmunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)
UseLentiviralTagsflagExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-BHMT-I/G
Plasmid#104442PurposeAutophagy cargo assayDepositorInsertBHMT (BHMT Human)
UseTagsGFP-myc GFP is from Aequorea victoria and GSTExpressionMammalianMutationN43A in GST (in a potential KFERQ-like sequence).…PromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-AP(-3)-Ub
Plasmid#232583PurposeExpress Ubiquitin tagged with a modified Acceptor Peptide (AP)DepositorInsertUbiquitin
UseTagsAPExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTomatoSlicer
Plasmid#221085PurposeDual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_XBB.1.5
Plasmid#196585Purposeexpressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus productionDepositorInsertSpike (XBB.1.5)
UseTagsExpressionMammalianMutationG252V, F486P on the basis of XBBPromoterCMVAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_NCOA4_RET
Plasmid#205865PurposeExpress mEGFP-tagged fusion protein, NCOA4_RET from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-PCAF
Plasmid#63705Purposeretroviral expression of human PCAF in mammalian cellsDepositorInsertPCAF (KAT2B Human)
UseRetroviralTagsNoneExpressionMammalianMutationE452GPromoterAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRuby-Golgi-7
Plasmid#55865PurposeLocalization: Golgi Complex, Excitation: 558, Emission: 605DepositorInsertGolgi (B4GALT1 Human)
UseTagsmRubyExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL410_INS421
Plasmid#49057PurposeExpresses luciferase under control of human insulin gene promoterDepositorInserthuman INS promoter (INS Human)
UseTagsExpressionMammalianMutationPromoterINS promoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only
