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Mammalian Expression

75,759 results

Showing: 1351 - 1400 of 75759 results

  2. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid
    #154868
    Purpose
    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry
    Depositor
    Ulrik Gether
    Has Service
    AAV Retrograde and AAV8
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV; Flp-frt
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Human Synapsin-1
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154868-plasmid-map-sequence-id-308827

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  4. pB-rtTA

    Plasmid
    #126034
    Purpose
    PiggyBac cargo vector expressing rtTA. Also confers resistance to G418.
    Depositor
    Mauro Calabrese
    Article
    Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do
    Insert
    rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hUbiC
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    126034-plasmid-map-sequence-id-245792

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  8. pRL-SV40P

    Plasmid
    #27163
    Purpose
    Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments
    Depositor
    Ron Prywes
    Article
    Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702
    Insert
    SV40 promoter
    Use
    Luciferase
    Tags
    Renilla Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2011
    Availability
    Academic Institutions and Nonprofits only
    27163-plasmid-map-sequence-id-419582

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  11. TFORF2536

    Plasmid
    #142397
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SOX6 (SOX6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    142397-plasmid-map-sequence-id-454976

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  16. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid
    #26976
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation
    Depositor
    Karl Deisseroth
    Has Service
    AAV5, AAV8, and AAV9
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    hSyn
    Available since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26976-plasmid-map-sequence-id-313556

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  21. GPR120-Tango

    Plasmid
    #66310
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR120 (FFAR4 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66310-plasmid-map-sequence-id-248122

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  23. pBS31-cCARLIN

    Plasmid
    #160928
    Purpose
    Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)
    Depositor
    Fernando Camargo
    Article
    Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.
    Insert
    CARLIN gRNAs and CAG-GFP-CARLIN array
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter for gRNAs; CAG promoter for GFP
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160928-plasmid-map-sequence-id-421629

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  29. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid
    #74707
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence
    Depositor
    Eugene Yeo
    Article
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Insert
    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    74707-plasmid-map-sequence-id-152737

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  31. pAAV-hSyn-fDIO-mCherry-WPREpA

    Plasmid
    #177328
    Purpose
    Control vector for Flp-dependent DREADD constructs
    Depositor
    Ulrik Gether
    Insert
    mCherry
    Use
    AAV; Flp-frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human Synapsin-1
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177328-plasmid-map-sequence-id-349583

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  32. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131462
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A K27M, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131462-plasmid-map-sequence-id-305183

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  35. pAK_DR30_CasRx_mCherry

    Plasmid
    #134844
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    134844-plasmid-map-sequence-id-266704

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  40. TFORF2886

    Plasmid
    #143655
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GLI3 (GLI3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    143655-plasmid-map-sequence-id-430148

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  46. pGF1-4eCOL2A1

    Plasmid
    #97210
    Purpose
    Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter
    Depositor
    Ryan Porter
    Article
    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d
    Insert
    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    COL2A1 regulatory elements
    Available since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    97210-plasmid-map-sequence-id-182599

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  49. eSpCas9(1.1)

    Plasmid
    #138555
    Purpose
    Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    eSpCas9(1.1)
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138555-plasmid-map-sequence-id-274761

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Showing: 1351 - 1400 of 75759 results