Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 21,502 results for: SPR

Clear keywords
Showing: 4161 - 4200 of 21502 results

  12. pFD115

    Plasmid
    #124768
    Purpose
    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Inserts
    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    Use
    CRISPR
    Promoter
    Ptet
    Available Since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124768-plasmid-map-sequence-id-246185

    Enlarge

  13. pBS SK mCherryROSAegfp

    Plasmid
    #54322
    Purpose
    Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.
    Depositor
    Silvestro Conticello
    Article
    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.
    Insert
    mCherry and EGFP
    Use
    CRISPR, Mouse Targeting, and TALEN
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    54322-plasmid-map-sequence-id-84459

    Enlarge

  14. dCas9-miniTurbo

    Plasmid
    #219822
    Purpose
    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    EF1a
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219822-plasmid-map-sequence-id-482202

    Enlarge

  23. pV1090

    Plasmid
    #111427
    Purpose
    gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)
    Depositor
    Gerald Fink
    Article
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    sgRNA-BsmBI stuffer
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111427-plasmid-map-sequence-id-212460

    Enlarge

  25. p-dCas9-TET1-Hygro

    Plasmid
    #104404
    Purpose
    transient expression of dCas9-TET1 fusion protein
    Depositor
    Stephan Beck
    Insert
    TET1 (TET1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104404-plasmid-map-sequence-id-204454

    Enlarge

  29. pYFP-NEAT1pr_v2-RT

    Plasmid
    #97088
    Purpose
    Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v2 vector, but also works with px330-NEAT1pr_v1.
    Depositor
    Archa Fox
    Article
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Insert
    NEAT1pr_v2-RT (NEAT1 Human)
    Use
    CRISPR
    Tags
    EYFP
    Promoter
    CMV
    Available Since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    97088-plasmid-map-sequence-id-189688

    Enlarge

  32. pCAT100

    Plasmid
    #180512
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180512-plasmid-map-sequence-id-359730

    Enlarge

  34. pUF1-dCas9-Sir2a

    Plasmid
    #122510
    Purpose
    Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.
    Depositor
    Lubin Jiang
    Article
    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116
    Insert
    dCas9-Sir2a
    Use
    CRISPR
    Tags
    3*FLAG
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available Since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122510-plasmid-map-sequence-id-243592

    Enlarge

  39. pYPQ220-Ultra

    Plasmid
    #176889
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d
    Insert
    AsCas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Plant
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    176889-plasmid-map-sequence-id-350058

    Enlarge

  40. pCAS9i

    Plasmid
    #141249
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141249-plasmid-map-sequence-id-308874

    Enlarge

Showing: 4161 - 4200 of 21502 results