165,688 results

1. pHR-TetOn-mCherry-OAZ1_FS-YFP

   Plasmid

   #232356

   Purpose

   Mammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)

   Depositor

   Ankur Jain

   Article

   Sharma et al bioRxiv [Preprint]. 2024 Nov 17:2024.08.

   Insert

   OAZ1 derived polyamine sensing module (OAZ1 Human)
   

   Use

   Lentiviral

   Tags

   eYFP and mCherry

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. VR122 pETv2 10His-pG-Tn5 (E54K, L372P)

   Plasmid

   #198467

   Purpose

   E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.

   Depositor

   Viviana Risca

   Article

   Soroczynski et al bioRxiv 2024.07.11.602973

   Insert

   10His-pG-Tn5 (E54K, L372P)
   

   Tags

   10His-pG

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   Consensus φ10 T7 Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Myosin-IIA-GFP

   Plasmid

   #38297

   Depositor

   Matthew Krummel

   Article

   Jacobelli et al J Immunol. 2009 Feb 15;182(4):2041-50.

   Insert

   Myosin II A (Myh9 Mouse)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1-EGFP

   Plasmid

   #19319

   Purpose

   3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ223 (dAsCpf1-SRDX)

   Plasmid

   #86209

   Purpose

   Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Insert

   dAsCpf1
   

   Use

   Gateway compatible cpf1 vectors

   Tags

   NLS and SRDX repressor and NLS

   Expression

   Plant

   Mutation

   D908A

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-NLS-HA-TurboID

   Plasmid

   #215075

   Purpose

   Expresses NLS-HA-TurboID in mammalian cells from a lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   TurboID (birA )
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. M50 Super 8x TOPFlash

   Plasmid

   #12456

   Purpose

   Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   TCF/LEF binding sites (Ctnnb1 )
   

   Use

   Luciferase

   Available Since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPLICS Mt-ER Short P2A

   Plasmid

   #164108

   Purpose

   Detect the short Mitochondria-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP Mt-ER Short P2A
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-GenEPi

   Plasmid

   #140236

   Purpose

   Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

   Depositor

   Periklis Pantazis

   Article

   Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

   Insert

   GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
   

   Tags

   GCaMP 6s RS1 EF4

   Expression

   Mammalian

   Promoter

   simian CMV IE94

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SPLICS Mt-ER Long P2A

    Plasmid

    #164107

    Purpose

    Detect the long Mitochondria-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP Mt-ER Long P2A
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW-Cas9-2A-EGFP

    Plasmid

    #167928

    Purpose

    Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Insert

    T2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    tTRE, hPGK

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209722

    Purpose

    Expresses mitochondrially targeted low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PE7-P2A-GFP

    Plasmid

    #222996

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-EGFP
    

    Tags

    P2A-EGFP, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCasPA

    Plasmid

    #113347

    Purpose

    Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7II-2N4Rtau

    Plasmid

    #177653

    Purpose

    Recombinant protein synthesis of human Tau

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    Tau (2N4R) (MAPT Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

    Plasmid

    #124429

    Purpose

    Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(TEV cleavage seq) T2A mCherry
    

    Expression

    Mammalian

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL1142 (pSPIN, pBBR1 backbone)

    Plasmid

    #160730

    Purpose

    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. scramble shRNA

    Plasmid

    #1864

    Purpose

    3rd gen lentiviral negative control vector containing scrambled shRNA

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    scramble
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-CreEGFP

    Plasmid

    #165140

    Purpose

    Expression of Cre recombinase fused with EGFP in C-terminal

    Depositor

    Daekee Lee, David Threadgill

    Insert

    Cre recombinase
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACRISPR

    Plasmid

    #113348

    Purpose

    A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pU6-pegRNA-GG-acceptor

    Plasmid

    #132777

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Exchangeable cassette
    

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. Rtn3L-mNeonGreen

    Plasmid

    #169034

    Purpose

    Express human Rtn3L in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rtn3L (RTN3 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLV-EF1a-IRES-Puro

    Plasmid

    #85132

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pYJA5

    Plasmid

    #217778

    Purpose

    The empty vector for quadruple sgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Puromycin resistance gene, T2A, and TagBFP

    Expression

    Mammalian

    Promoter

    PGK, CMV, U6

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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25. pInducer20

    Plasmid

    #44012

    Purpose

    Tet-inducible lentiviral vector for ORF expression

    Depositor

    Stephen Elledge

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEVS104

    Plasmid

    #207393

    Purpose

    Conjugal helper plasmid

    Depositor

    Jason Peters, Edward Ruby, Eric Stabb

    Article

    Hall et al MicroPubl Biol. 2023 Jul 13;2023:10.1791

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFlp-Km

    Plasmid

    #222334

    Purpose

    Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    

    Promoter

    cl, flp, and sacB

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. lentiCRISPRv2 puro

    Plasmid

    #98290

    Purpose

    Variant of lentiCRISPRv2 that confers puromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF-1a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

    Plasmid

    #221235

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
    

    Use

    Lentiviral

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pTXB1-nbMmIgG1-Tn5

    Plasmid

    #184287

    Purpose

    Anti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

    Depositor

    New York Genome Center, Ivan Raimondi

    Article

    Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

    Insert

    nbMmIgG1-Tn5
    

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-hSyn-GRAB-rDA3h

    Plasmid

    #208703

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3h
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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32. pcDNA-dCas9-p300 Core

    Plasmid

    #61357

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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33. LentiGuide Blast

    Plasmid

    #199622

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCW-Cas9

    Plasmid

    #50661

    Purpose

    Doxycycline-inducible lentiviral expression of SpCas9

    Depositor

    Eric Lander, David Sabatini

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    Tet ON

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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35. pH6-MBP-TEV-BirA

    Plasmid

    #179694

    Purpose

    Expresses His6 tagged MBP-BirA fusion protein

    Depositor

    Mark Arbing

    Insert

    BirA
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSIV-D3psi/delta env/delta Vif/delta Vpr

    Plasmid

    #132928

    Purpose

    Production of SIV-VLPs containing Vpx protein

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    Gag, pol, and vpx
    

    Use

    Lentiviral

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAKTaq

    Plasmid

    #25712

    Purpose

    Bacterial expression of Taq polymerase

    Depositor

    David Engelke

    Article

    Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

    Insert

    Taq polymerase
    

    Expression

    Bacterial

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 8xGTIIC-luciferase

    Plasmid

    #34615

    Purpose

    YAP/TAZ-responsive synthetic promoter driving luciferase expression

    Depositor

    Stefano Piccolo

    Article

    Dupont et al Nature. 2011 Jun 8;474(7350):179-83. doi

    Insert

    synthetic TEAD luciferase reporter
    

    Use

    Luciferase

    Available Since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. hNTo1-qgRNA-pYJA5

    Plasmid

    #217781

    Purpose

    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6, mouse U6, human H1, human 7SK

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. hNTo2-qgRNA-pYJA5

    Plasmid

    #217782

    Purpose

    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6, mouse U6, human H1, human 7SK

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge