161,592 results

1. pHH0103 IQGAP1 WW domain

   Plasmid

   #104279

   Purpose

   Bacterial expression of WW domain from IQGAP1

   Depositor

   Marius Sudol, Sachdev Sidhu

   Insert

   IQGAP1 WW domain (IQGAP1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX Socs3-SH2

   Plasmid

   #46506

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Socs3 (SOCS3 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 22-142

   Promoter

   Tac

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

   Plasmid

   #179430

   Purpose

   Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   TRE-cit-HS4-mch (SH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF alpha, pRSV

   Available since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. cmv-sfGFP-GB1-scAB

   Plasmid

   #185794

   Purpose

   scFv GFP compnent for SunRISER

   Depositor

   Robin Lee

   Article

   Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

   Insert

   sfGFP-GB1-scAB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX Socs1-SH2

   Plasmid

   #46504

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Socs1 (SOCS1 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-166

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEZYHb

   Plasmid

   #18669

   Purpose

   pKW32 converted Gateway Destination vector for expressing hemoglobin tagged fusion proteins in bacteria

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Use

   Tags

   hemoglobin

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. SPOP-V5 (BACK))

   Plasmid

   #169722

   Purpose

   Mammalian Expression of mouse SPOP Y353E

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   SPOP (Spop Mouse)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Y353E

   Promoter

   CMV

   Available since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPOP-V5 (BTB)

   Plasmid

   #169721

   Purpose

   Mammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   SPOP (Spop Mouse)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   L186D, L190D, L193D, I217K

   Promoter

   CMV

   Available since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPTn2P-PTK

   Plasmid

   #85604

   Purpose

   Passport transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

   Depositor

   Karl Clark, Scott Fahrenkrug

   Article

   Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

   Insert

   Passport transposon with a puromycin-thymidine kinase fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEW8

    Plasmid

    #84238

    Purpose

    Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae

    Depositor

    David Weinberg

    Article

    DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1

    Insert

    HA-mRuby-P2A-eGFP
    

    Use

    Tags

    HA-mRuby and eGFP

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Topo_SpCas9.tracr_AsCas12a.DR

    Plasmid

    #155050

    Purpose

    TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBFv-nosP-Cas9

    Plasmid

    #138402

    Purpose

    Expresses Cas9 in the germline of Drosophila

    Depositor

    Shu Kondo

    Article

    Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

    Insert

    Cas9-NLS
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. xGoosecoid

    Plasmid

    #24977

    Depositor

    Edward De Robertis

    Article

    Cho et al Cell. 1991 Dec 20. 67(6):1111-20.

    Insert

    Goosecoid (gsc-a Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

14. XP12 Xbra

    Plasmid

    #16978

    Depositor

    Randall Moon

    Insert

    Xbra (t.S Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pBHM2617

    Plasmid

    #234496

    Purpose

    NATR-CnU6-empty sgRNA

    Depositor

    Hiten Madhani

    Article

    Nalley et al bioRxiv 2025.02.14.638277

    Insert

    NAT-PCnU6-sgRNA_scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSP1D1-Strep-II

    Plasmid

    #224209

    Purpose

    Membrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.

    Depositor

    Anne Robinson

    Article

    Pettersen et al Biophys J. 2025 Jan 21;124(2):440-457. d

    Insert

    MSP1D1-Strep-II
    

    Use

    Tags

    Strep-II Tag

    Expression

    Bacterial

    Mutation

    Substitution of 7xHis tag found in pMSP1D1 for a …

    Promoter

    T7

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #236406

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-FRT/TO-APT2-LaG16-3W

    Plasmid

    #234425

    Purpose

    Express APT2 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

    Depositor

    William Fuller

    Article

    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

    Insert

    APT2 fused to non-binding mutant LaG16 (LYPLA2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-FRT/TO-APT1-LaG16-3W

    Plasmid

    #234424

    Purpose

    Express APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

    Depositor

    William Fuller

    Article

    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

    Insert

    APT1 fused to non-binding mutant LaG16 (LYPLA1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AiP12493 - pAAV-AiE0374h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2493)

    Plasmid

    #230214

    Purpose

    AiE0374h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. T2A 3xNLS GFP

    Plasmid

    #227721

    Purpose

    Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

    Depositor

    Haosheng Sun, Oliver Hobert

    Article

    Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

    Insert

    T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-6P-acatenin

    Plasmid

    #186458

    Purpose

    Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

    Depositor

    Tina Izard

    Article

    Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. DRH015_ AAV-hSyn-WT-THR

    Plasmid

    #225088

    Purpose

    Expression of wild-type thyroid receptor beta (THRB, human) with an HA tag in neurons driven by human synapsin promoter.

    Depositor

    Bernardo Sabatini

    Article

    Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

    Insert

    WT-THRB (THRB Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDRF1 mScarletI3-T2A-mTurquoise2

    Plasmid

    #219845

    Purpose

    equimolar expression of mScarletI3 and mTurquoise2 in budding yeast

    Depositor

    Bas Teusink

    Insert

    mScarletI3-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pHIV-EGFP.Flag-EZH2-mt1

    Plasmid

    #220244

    Purpose

    Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 1 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R27A, R31A, F165A

    Promoter

    EF-1α

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a(+)-SUMO-A'

    Plasmid

    #209115

    Purpose

    Bacterial expression of CC-GEMS ligand SUMO-A'

    Depositor

    Tom de Greef

    Article

    Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

    Insert

    SUMO-A'
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GpACR1-pcDNA3.1-mScarlet

    Plasmid

    #204964

    Purpose

    Expression of GpACR1 in fusion with mScarlet in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    GpACR1
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+ enhancer)

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AiP12214 - pAAV-ProB12-SYFP2-WPRE3-BGHpA (Alias: CN2214)

    Plasmid

    #208161

    Purpose

    AAV vector for SYFP2 expression in brain astrocytes

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-EF1a-DIO-mGFP-MutCREB(S133A)

    Plasmid

    #194642

    Purpose

    Expresses the fusion of monomeric-EGFP-tagged mutant CREB(S133A) in a Cre-dependent manner

    Depositor

    Jun-Hyeong Cho

    Article

    Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

    Insert

    CREB (Creb1 Rat)
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mutation

    S133A

    Promoter

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pETM33_CLIP_CAP_Gly

    Plasmid

    #178470

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    CLIP_CAP_Gly (CLIP1 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-HA-DEPTOR S286/287/291A

    Plasmid

    #159160

    Purpose

    Expresses DEPTOR S286/287/291A mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

    Insert

    DEPTOR (DEPTOR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed serine 286,287 and 291 to alanine

    Promoter

    CMV

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3-HA-DEPTOR S286/287/291D

    Plasmid

    #159161

    Purpose

    Expresses DEPTOR S286/287/291D mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

    Insert

    DEPTOR (DEPTOR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed serine 286,287 and 291 to aspartic acid

    Promoter

    CMV

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p206_LTJ_sgRNACD45.2_R3

    Plasmid

    #82674

    Purpose

    sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting CD45.2 region 3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV2/2

    Plasmid

    #104963

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes for production of serotype 2

    Depositor

    Melina Fan

    Insert

    Rep2/Cap2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p5

    Available since

    Oct. 2, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pAAV2/8

    Plasmid

    #112864

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap8
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Truncated P5

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28-MBP-super TEV protease

    Plasmid

    #171782

    Purpose

    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    MBP-sTEV
    

    Use

    Tags

    Arg6 tag and Internal His6 tag

    Expression

    Bacterial

    Mutation

    No internal TEV cleavage site between the MBP and…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCW57-CMV-ssRFP-GFP-KDEL

    Plasmid

    #128257

    Purpose

    Fluorescence reporter to monitor ER-phagy activity

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    ssRFPGFPKDEL
    

    Use

    Lentiviral

    Tags

    RFPGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLV-hTERT-IRES-hygro

    Plasmid

    #85140

    Purpose

    Lentiviral expression of hTERT, used to create immortalized cell lines

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. EGFP

    Plasmid

    #176015

    Purpose

    Mammalian expression of EGFP. Parton lab clone DFS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. v5 eVLP gag-pro-pol

    Plasmid

    #228466

    Purpose

    Expresses MMLVgag-pro-pol(Q226P) for producing v5 eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag-pro-pol(Q226P)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q226P

    Promoter

    CMV

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge