8,962 results

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pC0042-PguCas13b crRNA backbone

Plasmid

#103853

Purpose

For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

U6-PguCas13b DR-BbsI-BbsI-polyT

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21

Plasmid

#105519

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Promoter

T7

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-FLPo

Plasmid

#118027

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-DIO-FLPo

Use

AAV

Promoter

TRE

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Ai6

Plasmid

#22798

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

Insert

CAG-Floxed ZsGreen

Use

Mouse Targeting

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-PHLPP1 full length

Plasmid

#37100

Purpose

Expresses HA tagged, full-length human PHLPP1 in mammalian cells

Depositor

Alexandra Newton

Article

Warfel et al J Biol Chem. 2011 Jun 3;286(22):19777-88

Insert

PHLPP1 (PHLPP1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2-MT GLI2 delta N

Plasmid

#17649

Depositor

Erich Roessler

Article

Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

Insert

GLI2 (GLI2 Human)

Tags

6x Myc

Expression

Mammalian

Mutation

Missing 328 N-terminal amino acids

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCDNA3-STAT3-S727D

Plasmid

#73364

Purpose

Expression of STAT3-S727D

Depositor

Jie Chen

Article

Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

Insert

Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)

Tags

FLAG tagged

Expression

Mammalian

Mutation

E16K S727D

Promoter

CMV

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR AAA mutant

Plasmid

#13508

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR AAA (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Mutation

T24A S256A S319A

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc3-CAND1

Plasmid

#19948

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Tags

myc3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ACE2-GFP

Plasmid

#154962

Purpose

pcDNA3.1-ACE2-GFP

Depositor

Utpal Pajvani

Article

Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

Insert

ACE2-GFP (ACE2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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CoV2-N-R203M

Plasmid

#177952

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (R203M) (N )

Expression

Mammalian

Mutation

R203M

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Synapsin-cyto-mRuby3-iATPSnFR1.0

Plasmid

#102557

Purpose

expresses cytoplasmic ATP sensor in neurons with red reference protein

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

mRuby3-iATPSnFR1.0

Use

AAV

Tags

Ruby3

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pPS

Plasmid

#85801

Purpose

Backbone for pooled cloning of Perturb-seq libraries

Depositor

Aviv Regev

Article

Dixit et al Cell. 2016 Dec 15;167(7):1853-1866.e17.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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CHMP6_GFP

Plasmid

#31806

Depositor

Daniel Gerlich

Article

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

CHMP6 (CHMP6 Human)

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pGFP-EB1

Plasmid

#17234

Depositor

Lynne Cassimeris

Article

Piehl et al Mol Biol Cell. 2003 Mar . 14(3):916-25.

Insert

EB1 (MAPRE1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 11, 2008

Availability

Academic Institutions and Nonprofits only

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EF-hGPC3

Plasmid

#24670

Depositor

Jorge Filmus

Article

Gonzalez et al J Cell Biol. 1998 Jun 15. 141(6):1407-14

Insert

GPC3 (GPC3 Human)

Tags

HA

Expression

Mammalian

Available Since

May 6, 2010

Availability

Academic Institutions and Nonprofits only
pLKO-shPKM2_4

Plasmid

#42516

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

shRNA-PKM2 (PKM Human)

Use

Lentiviral

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA7

Plasmid

#26683

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 7 (KPNA7 Human)

Tags

T7

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223_HcRed

Plasmid

#25892

Purpose

Gateway entry vector containing HcRed.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

hcRed

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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mOrange-C1

Plasmid

#54680

Purpose

Localization: C1 Cloning Vector, Excitation: 548, Emission: 562

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mOrange

Expression

Mammalian

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcBac2.tR4-OMeYRS/GFP*

Plasmid

#50831

Purpose

baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

Depositor

Peter Schultz

Article

Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

Inserts

two-copy Tyr tRNA cassette
EGFP*
OMeYRS
two copy Tyr tRNA cassette

Use

Baculoviral

Tags

His, Myc-6xHis, and WPRE

Expression

Insect

Mutation

Y39TAG and able to charge various unnatural amino…

Promoter

CAG, CMV, and U6 and H1

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-AviTag-SnoopLigase

Plasmid

#105626

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

AviTag-SnoopLigase

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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DsRed-rab7 DN

Plasmid

#12662

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

DsRed and HA

Expression

Mammalian

Mutation

T22N

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pBabe K-Ras 12V

Plasmid

#12544

Depositor

Channing Der

Article

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

K-Ras 12V (KRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

12V constituitively activated

Available Since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pLPCX-NICD

Plasmid

#44471

Depositor

Ernesto Canalis

Article

Zanotti et al J Biol Chem. 2011 Feb 11;286(6):4576-88.

Insert

Notch intracellular domain (Notch1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Contains amino acid 1753 to C-Term

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only
pUC19-HRP-C

Plasmid

#40163

Depositor

Paul Ortiz de Montellano

Article

Hartmann et al Arch Biochem Biophys. 1992 Aug 15;297(1)

Insert

Horseradish peroxidase

Use

Coli cloning vector

Tags

Six histidine

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pSQT1313

Plasmid

#53370

Purpose

multiplex gRNA expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

None

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-myc3-NEDD8

Plasmid

#19943

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)

Tags

myc3

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pEGFPN1-human Dynamin 1aa

Plasmid

#22163

Depositor

Pietro De Camilli

Article

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa (DNM1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

D744N

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

Plasmid

#141383

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCI-MEG3

Plasmid

#44727

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

Maternally Expressed Gene 3 (MEG3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pmyc-GFP-TNRC6A

Plasmid

#41999

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Cdk6-HA

Plasmid

#1868

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Tags

HA

Expression

Mammalian

Mutation

D224Y

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
mADAM17 in pcDNA3.1

Plasmid

#19141

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM17 (Adam17 Mouse)

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCIneo RYR-1

Plasmid

#75113

Purpose

Clone encoding rabbit ryanodine receptor in mammalian expression vector

Depositor

Paul Allen, Robert Dirksen

Article

Nakai et al J Biol Chem. 1998 May 29;273(22):13403-6

Insert

Ryanodine receptor 1

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-mCh-Cry2olig

Plasmid

#101222

Purpose

Cry2olig(E490G)

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

Cry2olig

Use

Lentiviral

Expression

Mammalian

Mutation

E490G

Promoter

SFFV

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-SET-FLAG-HA

Plasmid

#24998

Depositor

Judy Lieberman

Article

Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

Insert

SET (SET Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 shSCR

Plasmid

#17920

Depositor

Sheila Stewart

Article

Saharia et al Curr Biol. 2008 Apr 8. 18(7):496-500.

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pQFlag-USP7 CS puroR

Plasmid

#46752

Purpose

Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

C223S--catalytically inactive

Promoter

CMV

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
R4pGWB601_UBQ10p-Turbo-YFP-NLS

Plasmid

#127368

Purpose

Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Tags

GS linker, NLS, V5, and YFP

Expression

Plant

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rab4B

Plasmid

#61801

Purpose

Human Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

Insert

Rab4B (RAB4B Human)

Tags

GFP

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS003-lenti-CMV-GCaMPer

Plasmid

#65227

Purpose

ER calcium sensor for lentivirus production

Depositor

Adam Cohen, Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

GCaMPer

Use

Lentiviral

Tags

circularly permuted GFP

Expression

Mammalian

Mutation

GCaMP3 (D324G, D360G, D397G, D435G)

Promoter

CMV

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEFLmGFP-Gli2

Plasmid

#37672

Depositor

Philip Beachy

Article

Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

Gli2 (Gli2 Mouse)

Tags

mGFP

Expression

Mammalian

Promoter

EF1a

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pROSA26-PA

Plasmid

#21271

Depositor

Frank Costantini

Article

Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

Insert

ROSA26 (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD104 (Peft-3::Cre)

Plasmid

#47551

Purpose

Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

Depositor

Bob Goldstein

Article

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Insert

Cre Recombinase

Use

Cre/Lox

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Pomc-pGL3

Plasmid

#17553

Purpose

Luciferase expression under control of Pomc promoter (–646 to +65).

Depositor

Domenico Accili

Article

Kitamura et al Nat Med. 2006 May . 12(5):534-40.

Insert

Pomc promoter (Pomc Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

Pomc

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-myc-Cdc42-wt

Plasmid

#12972

Depositor

Gary Bokoch

Insert

Cdc42 (CDC42 Human)

Tags

Myc

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Caspase1-flag

Plasmid

#21142

Depositor

Junying Yuan

Article

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Insert

caspase-1 (Casp1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
β3-integrin-YFP

Plasmid

#26653

Depositor

Jonathan Jones

Article

Tsuruta et al FASEB J. 2002 Jun . 16(8):866-8.

Insert

β3-integrin (ITGB3 Human)

Tags

YFP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
phage ubc nls ha 2xmcp HALO

Plasmid

#64540

Purpose

Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

MCP

Use

Lentiviral

Tags

HA, HALO, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,962 results