Skip to main content
Addgene
Showing: 6851 - 6900 of 157662 results

  8. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

    Plasmid
    #140005
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140005-plasmid-map-sequence-id-275488

    Enlarge

  16. pLenLox_U6 p53

    Plasmid
    #59360
    Purpose
    Lentiviral expression vector: p53 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.
    Depositor
    Peter Scheiffele
    Article
    Chih et al Science. 2005 Feb 25;307(5713):1324-8. E
    Inserts
    shRNA p53 (mouse) (Trp53 Mouse)
    eGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and mouse U6
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    59360-plasmid-map-sequence-id-93311

    Enlarge

  18. OA984

    Plasmid
    #120363
    Purpose
    Expresses an single chain antibody 1C19 that targets all four DENV serotypes and expresses a red fluorescent protein reporter
    Depositor
    Omar Akbari
    Article
    Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.
    Insert
    1C19 single chain antibody
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    AAEL010782 carboxypeptidase A (CPA) gene Promoter
    Available since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    120363-plasmid-map-sequence-id-234096

    Enlarge

  21. mEmerald-Integrin-Beta1-N-18

    Plasmid
    #54129
    Purpose
    Localization: Integrin/Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Integrin-Beta1 (ITGB1 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54129-plasmid-map-sequence-id-314085

    Enlarge

  23. pET21a-Streptavidin-Alive

    Plasmid
    #20860
    Purpose
    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Alive
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Wt gene
    Promoter
    Available since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20860-plasmid-map-sequence-id-194024

    Enlarge

  26. TFORF1456

    Plasmid
    #143811
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TFEC (TFEC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143811-plasmid-map-sequence-id-396257

    Enlarge

  29. pVP073

    Plasmid
    #222076
    Purpose
    eforRED visually marked spectinomycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al bioRxiv 2024.08.04.606528
    Type
    Empty backbone
    Use
    Chromoprotein-marked pvs1 backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222076-plasmid-map-sequence-id-440974

    Enlarge

  30. pVP074

    Plasmid
    #222077
    Purpose
    eforRED visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al bioRxiv 2024.08.04.606528
    Type
    Empty backbone
    Use
    Chromoprotein-marked pvs1 backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222077-plasmid-map-sequence-id-440969

    Enlarge

  31. pVP075

    Plasmid
    #222078
    Purpose
    AeBlue visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al bioRxiv 2024.08.04.606528
    Type
    Empty backbone
    Use
    Chromoprotein-marked pvs1 backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222078-plasmid-map-sequence-id-440967

    Enlarge

  35. LbCas12a-niCPE

    Plasmid
    #213747
    Purpose
    For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Insert
    MCP-nLbCas12a-M-MLV RT∆RNase H
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    D156R, R1138A in LbCas12a
    Promoter
    CMV
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213747-plasmid-map-sequence-id-422190

    Enlarge

  45. pBOB-VE-cadherin-tdTomato

    Plasmid
    #118733
    Purpose
    To express VE-cadherin tagged with tdTomato on C-terminus
    Depositor
    John Cooper
    Insert
    Cadherin 5 (CD144) (CDH5 Human)
    Use
    Lentiviral
    Tags
    tdTomato
    Expression
    Mutation
    I503T (Common SNP-see comments)
    Promoter
    CMV
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    118733-plasmid-map-sequence-id-229276

    Enlarge

Showing: 6851 - 6900 of 157662 results