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9,049 results

Showing: 7251 - 7300 of 9049 results

  8. pdCas9-DNMT3A-PuroR_v2

    Plasmid
    #74407
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74407-plasmid-map-sequence-id-140317

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  26. Clover2-N1

    Plasmid
    #54537
    Purpose
    Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Clover2
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54537-plasmid-map-sequence-id-127234

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  28. pdCas9::BFP-humanized

    Plasmid
    #44247
    Purpose
    Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 fused to BFP
    Use
    CRISPR and Retroviral
    Tags
    2xNLS & tagBFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    MSCV LTR promoter
    Available Since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44247-plasmid-map-sequence-id-60248

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  49. p52 cFlag pcDNA3

    Plasmid
    #20019
    Depositor
    Stephen Smale
    Insert
    Nfkb2 (Nfkb2 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Truncated at the p100 ligation site. It codes for…
    Available Since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
Showing: 7251 - 7300 of 9049 results