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Showing: 7451 - 7500 of 157662 results

  3. FLT3-D835Y-MSCV-IRES-GFP

    Plasmid
    #184780
    Purpose
    Expresses FLT3 D835Y activating tyrosine kinase domain mutant in mammalian cells
    Depositor
    Timothy Ley
    Insert
    FLT3 (FLT3 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Activating tyrosine kinase domain mutation (D835Y)
    Promoter
    MSCV
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    184780-plasmid-map-sequence-id-367916

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  8. pCDH-EF1-copGFP-T2A-Puro

    Plasmid
    #72263
    Purpose
    Express copGFP and puromycin resistance gene under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72263-plasmid-map-sequence-id-134557

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  10. TFORF2660

    Plasmid
    #142350
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    STAT1 (STAT1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142350-plasmid-map-sequence-id-397203

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  17. p663-UBC-PM-TurboID-V5_IDG-K

    Plasmid
    #214455
    Purpose
    Gateway destination clone of PM-TurboID (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    PM-TurboID-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214455-plasmid-map-sequence-id-423213

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  19. pDHt/sk-CE

    Plasmid
    #92128
    Purpose
    Expression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reesei
    Depositor
    Zhihua Zhou
    Article
    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1
    Insert
    Cas9-eGFP
    Use
    CRISPR; Trichoderma reesei expression
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Pcbh2
    Available since
    Nov. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92128-plasmid-map-sequence-id-195044

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  21. 4xmts-mNeonGreen

    Plasmid
    #98876
    Purpose
    In vivo visualization of mitochondria (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    4xmts
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    98876-plasmid-map-sequence-id-194881

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  22. pcDNA5 FRT/TO SF-HERC2 (ShB-R)

    Plasmid
    #55613
    Purpose
    Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells
    Depositor
    David Chan
    Article
    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.
    Insert
    3xFLAG tagged HERC2 (WT) (HERC2 Human)
    Use
    Tags
    3xFLAG and STREP
    Expression
    Mammalian
    Mutation
    Silent mutations have been incorporated into the …
    Promoter
    Available since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    55613-plasmid-map-sequence-id-86499

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  24. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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  27. HIV-1-GFP ΔEnv

    Plasmid
    #217437
    Purpose
    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.
    Depositor
    Jarrod Johnson
    Article
    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.
    Insert
    gag/pol
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    217437-plasmid-map-sequence-id-426708

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  29. mCherry-CD81-10

    Plasmid
    #55012
    Purpose
    Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    CD81 (CD81 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55012-plasmid-map-sequence-id-147992

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  37. pBad-EBFP2

    Plasmid
    #14891
    Purpose
    Bacterial expression of EBFP2 blue fluorescent protein
    Depositor
    Robert Campbell
    Article
    Ai et al Biochemistry. 2007 Apr 20. ():.
    Has Service
    Cloning Grade DNA
    Insert
    EBFP2
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    The fluorescent protein was inserted between XhoI…
    Promoter
    Available since
    May 15, 2007
    Availability
    Academic Institutions and Nonprofits only
    14891-plasmid-map-sequence-id-457487

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  39. pAD-LyseR

    Plasmid
    #99244
    Purpose
    For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)
    Depositor
    Jeff Hasty
    Article
    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102
    Insert
    phage lambda gene R (R phage lambda)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    beta-lactamase promoter
    Available since
    Oct. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99244-plasmid-map-sequence-id-191038

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  42. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

    Plasmid
    #26966
    Purpose
    AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition
    Depositor
    Karl Deisseroth
    Article
    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.
    Has Service
    AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    eNpHR 3.0
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    ER export at 3' end of gene. Trafficking sig…
    Promoter
    EF1alpha
    Available since
    Jan. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    26966-plasmid-map-sequence-id-200278

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  47. pGTMV

    Plasmid
    #118755
    Purpose
    Binary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.
    Depositor
    Jose-Antonio Daros
    Article
    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10
    Insert
    Tobacco mosaic virus
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Cauliflower mosaic virus 35S
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    118755-plasmid-map-sequence-id-229554

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  48. pTRKH2

    Plasmid
    #71312
    Purpose
    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.
    Depositor
    Todd Klaenhammer, Rodolphe Barrangou
    Article
    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.
    Type
    Empty backbone
    Use
    E. coli-gram positive bacteria shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    71312-plasmid-map-sequence-id-323567

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Showing: 7451 - 7500 of 157662 results