8,964 results

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pCMVHA E2F2

Plasmid

#24226

Purpose

Mammalian expression of human E2F2 with HA tag

Depositor

Kristian Helin

Article

Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

Insert

E2F2 (E2F2 Human)

Tags

HA

Expression

Mammalian

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pRSET FlipSuc-90u delta1

Plasmid

#14475

Depositor

Wolf Frommer

Article

Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

Insert

FlipThuE W283A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

N192D, W283A, 13 amino acids deleted from linkers

Promoter

T7

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-Arch-GFP

Plasmid

#22222

Purpose

Cre-dependent viral expression of Arch-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Has Service

AAV1 and AAV9

Insert

Arch

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

no

Promoter

CAG

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pHes1-GFPd2

Plasmid

#14808

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Hes1 promoter

Tags

GFPd2

Expression

Mammalian

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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AAVS1-Blasticidin-CAG-Flpe-ERT2

Plasmid

#68461

Purpose

AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

mammalian-codon-optimized Flpe recombinase fused with ERT2

Use

Synthetic Biology

Tags

ERT2

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-neo-RL-ZEB1

Plasmid

#35535

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB1 (ZEB1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR4-V5 (w71-3)

Plasmid

#17425

Purpose

Gateway entry vector for N-term V5 tag, frame A

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-14-3-3 ϵ

Plasmid

#48797

Purpose

expresses human myc-14-3-3ϵ in mammalian cells

Depositor

Huda Zoghbi

Article

Chen et al Cell. 2003 May 16;113(4):457-68.

Insert

YWHAE 14-3-3 ϵ (YWHAE Human)

Tags

haemagglutinin

Expression

Mammalian

Promoter

cmv

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
mNeptune2-C1

Plasmid

#54836

Purpose

Localization: C1 Cloning Vector, Excitation: 600, Emission: 650

Depositor

Michael Davidson

Type

Empty backbone

Tags

mNeptune2

Expression

Mammalian

Available Since

June 11, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5-myc-Cdc42-T17N

Plasmid

#12973

Depositor

Gary Bokoch

Insert

Cdc42 DN (CDC42 Human)

Tags

Myc

Expression

Mammalian

Mutation

T17N Dominant negative

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Rheb1 shRNA #1

Plasmid

#26625

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Rheb1 shRNA (RHEB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
TroponinT-GCaMP5-Zeo

Plasmid

#46027

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GCaMP5-2A-Zeo

Use

Lentiviral

Expression

Mammalian

Promoter

TNNT2

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP3Kv-WPRE

Plasmid

#132332

Purpose

expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3Kv

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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AAV CAMKII PSAM4 GlyR IRES EGFP

Plasmid

#119744

Purpose

Chemogenetic inhibitor expression plasmid

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has Service

AAV5

Insert

PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)

Use

AAV

Expression

Mammalian

Mutation

L131G, Q139L, Y217F

Promoter

CamKII

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pKCcas9dO

Plasmid

#62552

Purpose

High efficiency Streptomyces genome editing by CRISPR/Cas9 system

Depositor

Weihong Jiang

Article

Huang et al Acta Biochim Biophys Sin (Shanghai). 201

Inserts

Scocas9
sgRNA ()
act-orf4 homology arm

Use

CRISPR; Bacterial genome editing

Expression

Bacterial

Promoter

j23119 and ptipA

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40

Plasmid

#105551

Purpose

AAV expression of GFP-Cre from CamKII promoter

Depositor

James M. Wilson

Has Service

AAV Retrograde and AAV9

Insert

EGFP-Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CamKII

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK3 wt

Plasmid

#80873

Purpose

mammalian expression of ALK3 WT

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK3 (BMPR1A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pSKDuet01

Plasmid

#12172

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

NpuDnaE

Tags

His-GB1

Expression

Bacterial

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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miniSOG-N1

Plasmid

#54824

Purpose

Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Tags

miniSOG

Expression

Mammalian

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBabe-puro-IMS-RP

Plasmid

#24535

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

Diablo homolog (DIABLO Human)

Use

Retroviral

Tags

mRFP1

Expression

Mammalian

Mutation

Deleted residues 56-239

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA Flag-Smad4M

Plasmid

#14959

Depositor

Joan Massague

Article

Kang et al Proc Natl Acad Sci U S A. 2005 Sep 27. 1

Insert

Smad4 (SMAD4 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_iSH2-pMag(3x)-iRFP

Plasmid

#67304

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

iSH2-pMag(3x)-iRFP (PIK3R1 Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRetroSuper-shSrc

Plasmid

#26982

Depositor

Joan Massague

Article

Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

Insert

shRNA Src (SRC Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pMKO.1 puro p53 shRNA 2

Plasmid

#10672

Depositor

William Hahn

Article

Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

Insert

p53 shRNA (TP53 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pAd-M3cherry

Plasmid

#61041

Purpose

This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.

Depositor

Qiao Zhou

Article

Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.

Inserts

Ngn3 (Neurog3 Mouse)
pdx1 (Pdx1 Mouse)
Mafa (Mafa Mouse)
monomeric cherry

Use

Adenoviral

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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LeGO-lnc

Plasmid

#80624

Purpose

Lentiviral lncRNA Overexpression

Depositor

Jan-Henning Klusmann

Article

Emmrich et al Mol Cancer. 2014 Jul 15;13:171. doi: 10.

Insert

empty control

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM33_Nsp5_Mpro

Plasmid

#156475

Purpose

Bacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp5_Mpro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+8CmCherry

Plasmid

#34935

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Promoter

SP6

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-H-Ras_V12

Plasmid

#39504

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCVgfp::AID

Plasmid

#15925

Depositor

Nina Papavasiliou

Article

Dickerson et al J Exp Med. 2003 May 19. 197(10):1291-6.

Insert

AID (AICDA Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
paavCAG-JxON-pre-mGRASP-mCerulean

Plasmid

#51903

Purpose

JxOn-pre

Depositor

Jinhyun Kim

Article

Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Promoter

CAG

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMN YFP-LID

Plasmid

#31767

Depositor

Thomas Wandless

Article

Bonger et al Nat Chem Biol. 2011 Jul 3. doi: 10.1038/

Insert

YFP-LID (FKBP1A Human)

Use

Retroviral

Tags

HA and YFP

Mutation

F36V on FKBP12 and additional 19 aa (TRGVEEVAEGVV…

Promoter

LTR

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pFlpStop-attB-UAS-2.1-tdTom

Plasmid

#88910

Purpose

attB FlpStop plasmid, for insertion into MiMIC site

Depositor

Tom Clandinin

Article

Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

Insert

FlpStop

Expression

Insect

Promoter

5XUAS

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPhiC31o

Plasmid

#13794

Purpose

Expression of mouse codon-optimized PhiC31 recombinase

Depositor

Philippe Soriano

Article

Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

Insert

PhiC31o

Expression

Bacterial

Available Since

Jan. 16, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti CMV p16 Neo (w111-1)

Plasmid

#22260

Depositor

Eric Campeau

Insert

cyclin-dependent kinase inhibitor 2A (CDKN2A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-Rac1

Plasmid

#11391

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Tags

YFP

Expression

Mammalian

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-Gsdmd-CT

Plasmid

#80953

Purpose

Expresses Gsdmd-CT in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-CT (Gsdmd Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1alpha-SARS-CoV-2-nsp11-2xStrep-IRES-Puro

Plasmid

#141377

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp11 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMJ826

Plasmid

#39316

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Histidine 840 to Alanine (H840A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-Elongin C-pcDNA3

Plasmid

#19998

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

Elongin C (ELOC Human)

Tags

T7-tag

Expression

Mammalian

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-mLin28A

Plasmid

#26357

Depositor

George Daley

Article

Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

Insert

lin-28 homolog A (Lin28a Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pZLCv2-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106357

Purpose

Empty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitation

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3XFLAG and HA

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe 12S E1A

Plasmid

#18742

Depositor

Scott Lowe

Article

Samuelson et al Proc Natl Acad Sci U S A. 1997 Oct 28. 9

Insert

12S E1A

Use

Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)

Plasmid

#145843

Purpose

Lentiviral vector genome to ectopically express human TMPRSS2 variant 1

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human TMPRSS2 variant 1 (TMPRSS2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G8V

Promoter

SFFV

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-GFP-MMP7

Plasmid

#11989

Depositor

Steven Johnson

Insert

MMP7 (MMP7 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY2(L348F)-CreN

Plasmid

#75368

Purpose

Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Cre recombinase, residues 19-104

Expression

Mammalian

Mutation

L348F

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLM-mCherry-Klf4

Plasmid

#23243

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)

Use

Lentiviral

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD/His-B-iRFP

Plasmid

#31855

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Expression

Bacterial

Mutation

S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…

Promoter

araBAD

Available Since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-HA-MST2

Plasmid

#33098

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

MST2 (STK3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
WT-eNOS-RFP

Plasmid

#22497

Depositor

William Sessa

Article

Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

Insert

WT eNOS

Tags

RFP

Expression

Mammalian

Available Since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

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8,964 results