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173,766 results

Showing: 7551 - 7600 of 173766 results

  2. pNX24-RFP-MS2SL

    Plasmid
    #227819
    Purpose
    Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene
    Depositor
    Jeffrey Gerst
    Article
    Haimovich et al bioRxiv 2024.11.06.622258
    Inserts
    3xHA-RFP-24xMS2SL
    Neo resistance
    Expression
    Mammalian
    Promoter
    CMV and SV40
    Available Since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    227819-plasmid-map-sequence-id-469648

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  3. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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  7. MCP-eN3x9

    Plasmid
    #210706
    Purpose
    This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210706-plasmid-map-sequence-id-416266

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  8. pGL4.22-VEGF-HRE::dLUC

    Plasmid
    #128096
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Promoter
    inserted VEGF hypoxia response elements (HRE) (x5)
    Available Since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128096-plasmid-map-sequence-id-249669

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  15. qTAG-N-Solo-mStayGold-LMNB1

    Plasmid
    #227328
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a mStayGold Tag (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227328-plasmid-map-sequence-id-451726

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  17. qTAG-N-Solo-mScarlet-ACTB

    Plasmid
    #207750
    Purpose
    Donor template for mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a mScarlet Tag (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207750-plasmid-map-sequence-id-414814

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  18. pCUU

    Plasmid
    #159747
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159747-plasmid-map-sequence-id-313815

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  23. pSL21-VEX

    Plasmid
    #158230
    Purpose
    Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)
    Depositor
    Junwei Shi
    Article
    Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Expression
    Mammalian
    Promoter
    U6 promoter for crRNA expression and EFS promoter…
    Available Since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158230-plasmid-map-sequence-id-317503

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  32. mycBioID2-13X Linker-MCS

    Plasmid
    #92308
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    Humanized A. aeolicus BioID2 with R40G and Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    92308-plasmid-map-sequence-id-216421

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  33. pAAV.GFAP.Cre.WPRE.hGH

    Plasmid
    #105550
    Purpose
    AAV expression of Cre from GFAP promoter
    Depositor
    James M. Wilson
    Has Service
    AAV PHP.eB and AAV5
    Insert
    Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    GFAP
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105550-plasmid-map-sequence-id-202448

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  37. pEGFP GR

    Plasmid
    #47504
    Purpose
    Expresses glucocorticoid receptor in mammalian cells
    Depositor
    Alice Wong
    Insert
    Glucocorticoid receptor (NR3C1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    47504-plasmid-map-sequence-id-223484

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  45. pJL-TRBO-G

    Plasmid
    #80083
    Purpose
    An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence and expresses GFP.
    Depositor
    John Lindbo
    Article
    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.
    Insert
    GFP
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Nov. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    80083-plasmid-map-sequence-id-372997

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  46. pUltra-Plus

    Plasmid
    #161775
    Purpose
    3rd generation lentiviral multicistronic vector for mammalian expression of three genes of interest
    Depositor
    Jorge Luis Arias-Arias
    Type
    Empty backbone
    Use
    Lentiviral; Multicistronic
    Expression
    Mammalian
    Promoter
    UbC
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161775-plasmid-map-sequence-id-316822

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  48. pcDNA3-TLR2-YFP

    Plasmid
    #13016
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 2 (TLR2 Human)
    Tags
    YFP
    Expression
    Mammalian
    Available Since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13016-plasmid-map-sequence-id-472886

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Showing: 7551 - 7600 of 173766 results