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159,111 results

Showing: 9001 - 9050 of 159111 results

  6. pETMCSI-T4P_Y320A

    Plasmid
    #122522
    Purpose
    Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis
    Depositor
    Gottfried Otting
    Article
    Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do
    Insert
    gp43 - DNA polymerase (43 Escherichia virus T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tyrosine 320 changed to alanine
    Promoter
    T7 promoter
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122522-plasmid-map-sequence-id-238200

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  7. FUGW-d2GFP-ZEB1 3'UTR

    Plasmid
    #79601
    Purpose
    FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.
    Depositor
    Jeffrey Rosen
    Article
    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.
    Insert
    d2GFP-Zeb1 3'UTR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    79601-plasmid-map-sequence-id-398021

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  8. mTagRFP-Membrane-1

    Plasmid
    #57992
    Purpose
    Localization: Plasma Membrane, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    N-terminal 20 Aas MLS from Neuromodulin and mTagR…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    57992-plasmid-map-sequence-id-313444

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  11. pLenti.PGK.LifeAct-Ruby.W

    Plasmid
    #51009
    Purpose
    Lentivector that expresses LifeAct-Ruby from internal human PGK promoter
    Depositor
    Rusty Lansford
    Insert
    LifeAct-mRFPruby
    Use
    Lentiviral
    Tags
    LifeAct
    Expression
    Mammalian
    Mutation
    Promoter
    human PGK
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51009-plasmid-map-sequence-id-339916

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  21. AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR

    Plasmid
    #191795
    Purpose
    vector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13d
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    A134V, A140V, A141V, A143V
    Promoter
    EFS, hU6
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191795-plasmid-map-sequence-id-375580

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  26. ITGAV-bio-His

    Plasmid
    #51919
    Purpose
    Expresses full-length Integrin alpha-V precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ITGAV (ITGAV Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51919-plasmid-map-sequence-id-79896

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  31. IRES-creERT2-FNF

    Plasmid
    #188230
    Purpose
    EMCV IRES-cre-ERT2-FRT-Neo*-FRT cassette
    Depositor
    Mario Capecchi
    Insert
    IRES-creERT2-FNF
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    188230-plasmid-map-sequence-id-371215

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  33. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid
    #185914
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185914-plasmid-map-sequence-id-367363

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  37. pCS2-Opto-zFGFR1a

    Plasmid
    #232639
    Purpose
    Zebrafish FGFR1a receptor kinase domain fused to VfLOV
    Depositor
    Katherine Rogers
    Insert
    zFGFR1a kinase domain + VfLOV domain
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    232639-plasmid-map-sequence-id-461337

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  38. ADRA2B-Tango

    Plasmid
    #66217
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRA2B (ADRA2B Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  47. pcDNA-3-VEGFR3

    Plasmid
    #119230
    Purpose
    Plasmid encodes full-length human VEGFR-3 gene cloned in mammalian expression vector.
    Depositor
    Kari Alitalo
    Insert
    VEGFR3 (FLT4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119230-plasmid-map-sequence-id-231667

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  48. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

    Plasmid
    #216156
    Purpose
    Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216156-plasmid-map-sequence-id-437768

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Showing: 9001 - 9050 of 159111 results