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161,873 results

Showing: 9001 - 9050 of 161873 results

  4. PB CAG-tdTomato

    Plasmid
    #133569
    Purpose
    CAG tdTomato piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133569-plasmid-map-sequence-id-274028

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  5. TFORF2165

    Plasmid
    #141953
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GRHL3 (GRHL3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    141953-plasmid-map-sequence-id-391431

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  6. pSBBi-Pur GFP-TM

    Plasmid
    #139185
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.
    Depositor
    Neal Devaraj
    Insert
    GFP-TM
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    fused to membrane anchor
    Promoter
    EF1α
    Available since
    March 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    139185-plasmid-map-sequence-id-309371

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  9. pKP864

    Plasmid
    #167658
    Purpose
    Express colicinB-His6
    Depositor
    Kathleen Postle
    Insert
    cba-his6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167658-plasmid-map-sequence-id-337304

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  14. pET StrepII TEV LIC cloning vector (1R)

    Plasmid
    #29664
    Depositor
    Scott Gradia
    Type
    Empty backbone
    Use
    Tags
    Strep II and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29664-plasmid-map-sequence-id-13779

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  15. pANAP

    Plasmid
    #48696
    Purpose
    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS
    Depositor
    Peter Schultz
    Article
    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540
    Inserts
    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutant E.coli leucyl synthetase specific for Anap…
    Promoter
    CMV and human H1 promoter
    Available since
    Nov. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    48696-plasmid-map-sequence-id-197837

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  18. pDB-His-MBP-SARM1

    Plasmid
    #195762
    Purpose
    Purification of SARM1 from E.coli
    Depositor
    Hao Wu
    Insert
    SARM1 (SARM1 Human)
    Use
    Tags
    HIS-MBP-TEV
    Expression
    Bacterial
    Mutation
    truncation of first 19 residues
    Promoter
    T7
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195762-plasmid-map-sequence-id-384751

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  19. mCherry-Centrin2-N-10

    Plasmid
    #55018
    Purpose
    Localization: Centrosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Centrin2 (CETN2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55018-plasmid-map-sequence-id-371039

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  20. pRK5-myc-Rac1-Q61L

    Plasmid
    #12983
    Depositor
    Gary Bokoch
    Insert
    Rac1 constitutively active (RAC1 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Q61L Constitutively active
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    12983-plasmid-map-sequence-id-154551

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  24. TFORF2886

    Plasmid
    #143655
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GLI3 (GLI3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    143655-plasmid-map-sequence-id-430148

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  25. pcDNA3-Lyn-dPlcR

    Plasmid
    #181832
    Purpose
    Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).
    Depositor
    Jin Zhang
    Article
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Insert
    Lyn-PlcR
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181832-plasmid-map-sequence-id-364041

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  29. pJE53

    Plasmid
    #194153
    Purpose
    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.
    Depositor
    Edward Geisinger
    Article
    Bai et al J Bacteriol. 2021 May 20;203(12):e005652
    Insert
    sgRNA with mrfp control guide sequence
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    194153-plasmid-map-sequence-id-382435

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  32. kiCAP-AAV-MDV1A

    Plasmid
    #196679
    Purpose
    Rep/Cap plasmid for the production of MDV1A, an AAV capsid with CNS tropism in mice.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RSVGSVY insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    196679-plasmid-map-sequence-id-386954

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  35. 5aa SunTag VP64 CLV3

    Plasmid
    #115485
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    115485-plasmid-map-sequence-id-238830

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  39. pAV hH1-ARRET

    Plasmid
    #111374
    Purpose
    ARRET expression contains approximately 130 anti-sense telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences
    Depositor
    Klaus Holzmann
    Insert
    ARRET w/o polyA
    Use
    Adenoviral
    Tags
    Expression
    Mutation
    Promoter
    hH1
    Available since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    111374-plasmid-map-sequence-id-209217

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  40. pAV10.F3

    Plasmid
    #63199
    Purpose
    BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63199-plasmid-map-sequence-id-108468

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  46. pLV-EF1A-CD19.28H.28TM.BB.3z

    Plasmid
    #200679
    Purpose
    Modular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Article
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD28HD-CD28TM-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-short
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    200679-plasmid-map-sequence-id-392606

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  47. mCherry-Rab4a-7

    Plasmid
    #55125
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Rab4a (RAB4A Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55125-plasmid-map-sequence-id-129652

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  49. 5aa SunTag VP64 AP3

    Plasmid
    #115484
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    115484-plasmid-map-sequence-id-238822

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Showing: 9001 - 9050 of 161873 results