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173,227 results

Showing: 9001 - 9050 of 173227 results

  3. pDEST51-LRRK2-G2019S

    Plasmid
    #29401
    Depositor
    Mark Cookson
    Insert
    LRRK2 (LRRK2 Human)
    Use
    Gateway Cloning
    Tags
    6XHis and V5
    Expression
    Mammalian
    Mutation
    Gly2019Ser
    Available Since
    April 1, 2011
    Availability
    Academic Institutions and Nonprofits only
    29401-plasmid-map-sequence-id-256667

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  5. mCherry-LaminA-C-18

    Plasmid
    #55068
    Purpose
    Localization: Nuclear Envelope, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Lamin A (LMNA Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55068-plasmid-map-sequence-id-200503

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  19. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Richard Cooley, Ryan Mehl
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available Since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

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  23. pTRC-LIC

    Plasmid
    #62343
    Purpose
    Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    tac promoter from pKK233-2
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62343-plasmid-map-sequence-id-102266

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  25. pgRNA-humanized

    Plasmid
    #44248
    Purpose
    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    guide RNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    mouse U6
    Available Since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44248-plasmid-map-sequence-id-190219

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  35. pCAG-memRFP

    Plasmid
    #224564
    Purpose
    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    mRFP1
    Use
    Unspecified
    Tags
    Membrane localization signal
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224564-plasmid-map-sequence-id-451482

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  36. pcDNA3.3_SARS2_XBB.1.5

    Plasmid
    #196585
    Purpose
    expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XBB.1.5)
    Expression
    Mammalian
    Mutation
    G252V, F486P on the basis of XBB
    Promoter
    CMV
    Available Since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196585-plasmid-map-sequence-id-386779

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  43. mCherry-Kinesin11-N-18

    Plasmid
    #55067
    Purpose
    Localization: Motor Protein/Microtubule Filaments, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Kinesin11 (KIF11 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55067-plasmid-map-sequence-id-244069

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  44. DT12A

    Plasmid
    #80416
    Purpose
    Expresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats
    Depositor
    Thomas Cooper
    Article
    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub
    Insert
    human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
    Expression
    Mammalian
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80416-plasmid-map-sequence-id-457373

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  47. pTSlb-R756K

    Plasmid
    #60731
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756S
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available Since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60731-plasmid-map-sequence-id-99358

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Showing: 9001 - 9050 of 173227 results