162,408 results

1. IDP432

   Plasmid

   #87000

   Purpose

   plasmid for cell culture expression of SYNseq components

   Depositor

   Anthony Zador

   Article

   Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

   Insert

   SNAP-Nlg1AB-1xλN(i)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-DN-hCUL4A-FLAG

   Plasmid

   #15821

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin4A (CUL4A Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N440

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Px461-Cas9n-Trp53-sgRNA-beta

   Plasmid

   #88847

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pPB-EF1A-Puro-hTBK1-EGFP

   Plasmid

   #221553

   Purpose

   Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Piggybac

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1_Lifeact-EGFP

   Plasmid

   #67303

   Purpose

   Fluorescent indicator for F-actin

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

   Insert

   Lifeact-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-scFv-T

   Plasmid

   #67843

   Purpose

   pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

   Depositor

   Joanna Bereta

   Article

   Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

   Insert

   antibody 1D8 light kappa chain
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Mito-CanlonicSF/pCMV-myc-mito

   Plasmid

   #224465

   Purpose

   To explore mitochondrial lactate dynamics

   Depositor

   Alejandro San Martín

   Article

   Rauseo et al bioRxiv 2024.07.19.604361

   Insert

   CanlonicSF
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pFLP-hyg

   Plasmid

   #87831

   Purpose

   Expresses FLP recombinase with hyg selection marker

   Depositor

   Pep Charusanti

   Article

   Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

   Insert

   hyg
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   None

   Promoter

   heat-shock inducible

   Available since

   Aug. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC19/PLIN2 GFP-P2A-BFP

   Plasmid

   #202195

   Purpose

   knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   Cloning vector

   Tags

   GFP-P2A-BFP knock-in

   Expression

   Mutation

   Promoter

   lac

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-HA-DEPTOR-WT

    Plasmid

    #159159

    Purpose

    Expresses DEPTOR in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

    Insert

    DEPTOR (DEPTOR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid

    #188899

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pWN_U6-B2M-miniGag-Cas9

    Plasmid

    #228958

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMRX-INU-FLAG-FAM134B

    Plasmid

    #128260

    Purpose

    Expresses FAM134B in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    FAM134B (RETREG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Cas9 sgRNA vector

    Plasmid

    #68463

    Purpose

    Cas9 sgRNA vector for cloning

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)

    Plasmid

    #128608

    Purpose

    Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    36xMashTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GST-Pin1

    Plasmid

    #19027

    Depositor

    Michael Yaffe

    Article

    Yaffe et al Science. 1997 Dec 12. 278(5345):1957-60.

    Insert

    Pin1 (PIN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2_hMGFP-60A

    Plasmid

    #200393

    Purpose

    A plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

    Depositor

    Xiao Wang

    Article

    Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

    Insert

    monster GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV_S5E2_Chrimson_tdTomato

    Plasmid

    #219432

    Purpose

    AAV construct expressing Chrimson-tdTomato under the control of E2 regulatory element

    Depositor

    Gordon Fishell

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Insert

    Chrimson-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3/BLM

    Plasmid

    #111766

    Purpose

    To express human BLM protein in human cells

    Depositor

    Ian Hickson

    Article

    Gaymes et al Oncogene. 2002 Apr 11;21(16):2525-33. do

    Insert

    BLM (BLM Human)
    

    Use

    Tags

    Flag epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiPGK DEST H2B-iRFP670

    Plasmid

    #90237

    Purpose

    Lentiviral vector to express H2B-iRFP670 under PGK promoter

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    H2B
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid

    #133451

    Purpose

    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    TfR-sfGFP-myc tag-SpyCatcher003
    

    Use

    Tags

    GSSGS and myc tag

    Expression

    Mammalian

    Mutation

    Contains C20 and A23 mutations that improve plasm…

    Promoter

    CMV

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TFORF1983

    Plasmid

    #143562

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TBX5 (TBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAID6

    Plasmid

    #136989

    Purpose

    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Inserts

    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO2, TDH3, TEF1, and TPI1

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-CaMKII-ChrimsonR-tdTomato

    Plasmid

    #99231

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    CaMKII

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV10/3xflag-Clock

    Plasmid

    #47334

    Purpose

    full length clock in pCMV10 with 3x flag at the N-terminus

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    clock
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Px461-Cas9n-Trp53-sgRNA-alpha

    Plasmid

    #88846

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

27. pFLAG-HHARI

    Plasmid

    #17450

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    HHARI (ARIH1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFGAMS-EGFP

    Plasmid

    #99107

    Purpose

    Expresses human PFAS-EGFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    PFAS (PFAS Human)
    

    Use

    Tags

    EGFP (enhanced green fluorescent protein)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHIV-EGFP.Flag-EZH2-mt2

    Plasmid

    #220245

    Purpose

    Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

    Promoter

    EF-1α

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVX-LC3-YFP

    Plasmid

    #99571

    Purpose

    Lentiviral expression of YFP-tagged LC3

    Depositor

    Edward Campbell

    Article

    Flavin et al Acta Neuropathol. 2017 May 19. doi: 10.1

    Insert

    LC3 (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CPSF5

    Plasmid

    #155480

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CPSF5 (NUDT21 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAG-mNaChBac-T2A-tdTomato

    Plasmid

    #60650

    Purpose

    Expresses mNaChBac-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    mNaChBac-T2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-FLEX-SaCas9-U6-sgSlc17a6

    Plasmid

    #124847

    Purpose

    Mutagenesis of Slc17a6 with SauCas9

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a6 gRNA (Slc17a6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLJM1-FLAG-GFP-OSBP

    Plasmid

    #134659

    Purpose

    Flag and GFP-tagged OSBP

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    OSBP (OSBP Human)
    

    Use

    Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCSDest-HA-TMPRSS2

    Plasmid

    #154963

    Purpose

    TMPRSS2 with N-terminal HA

    Depositor

    Utpal Pajvani

    Article

    Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

    Insert

    HA-TMPRSS2 (TMPRSS2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pcDNA-iLACCO1.2

    Plasmid

    #218823

    Purpose

    Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

    Depositor

    Robert Campbell

    Article

    Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

    Insert

    iLACCO1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDEST-FLAG

    Plasmid

    #183511

    Purpose

    Gateway converted, destination vector with N-terminal Flag tag. For mammalian expression.

    Depositor

    Emanuele Panza

    Article

    Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

    Type

    Empty backbone

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAcGHLT-B-DDB1

    Plasmid

    #48638

    Purpose

    Expresses Full Lenght DDB1 in Insect Cells (ampR)

    Depositor

    Ning Zheng

    Article

    Li et al Cell. 2006 Jan 13;124(1):105-17.

    Insert

    DDB1 (DDB1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

39. gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1)

    Plasmid

    #217345

    Purpose

    crRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
    crCLTA-4 gRNA: ggctctgcaacaccgcctagacc (CLTA Human)
    crFOLH1-1 gRNA: gctccagacctggggtccagttt (FOLH1 Human)
    crCD151-3 gRNA: cgggaggccgcacccaccgcctg (CD151 Human)
    crHBG-3 gRNA: ttcttcatccctagccagccgcc (HBG1, HBG2 Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. FusionRed-Fascin-C-10

    Plasmid

    #56117

    Purpose

    Localization: Cytoskeleton, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    Fascin (FSCN1 Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

41. Desmoplakin I tension sensor (F40-based)

    Plasmid

    #119186

    Purpose

    The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.

    Depositor

    Alex Dunn

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
    

    Use

    Transposon

    Tags

    mTFP1-F40-mEYFP

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    TCE

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189925

    Purpose

    AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SauriABE8e
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SauriCas9 D15A

    Promoter

    EFS

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pNC02

    Plasmid

    #233644

    Purpose

    For bacterial expression of sfGFP c-terminally tagged with VitelloTagB.

    Depositor

    Zak Swartz

    Article

    Clarke et al Development. 2024 Oct 15;151(20):dev2028

    Insert

    sfGFP with c-terminal VitelloTagB
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCAG mitoYFP

    Plasmid

    #168508

    Purpose

    Expression of mito-EYFP (mitochondrial matrix targeted EYFP) for visualization of mitochondrial dynamics

    Depositor

    Franck Polleux

    Article

    Lewis TL et al Curr Biol. 2016 Oct 10;26(19):2602-2608.

    Insert

    mitoYFP
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. sfGFP-N1

    Plasmid

    #54737

    Purpose

    Localization: N1 Cloning Vector, Excitation: 485, Emission: 507

    Depositor

    Geoffrey Waldo, Michael Davidson

    Article

    Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

    Type

    Empty backbone

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. TFORF1218

    Plasmid

    #142157

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PRRX1 (PRRX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1

    Plasmid

    #138461

    Purpose

    lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    MCP-VP64-IRES-zsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. NLS-Laconic/pCMV-myc-nuc

    Plasmid

    #46307

    Purpose

    a nuclear-targeted, genetically-encoded sensor for lactate

    Depositor

    Luis Felipe Barros

    Article

    Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

    Insert

    NLS-Laconic
    

    Use

    Lactate biosensor

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. TFORF2908

    Plasmid

    #143663

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFIC (NFIC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. Green Glifon600

    Plasmid

    #126207

    Purpose

    green fluorescent glucose sensor for mammalian cells

    Depositor

    Tetsuya Kitaguchi

    Article

    Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

    Insert

    Green Glifon600
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge