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177,170 results

Showing: 9351 - 9400 of 177170 results

  1. alpha catenin conformation sensor

    Plasmid
    #71709
    Purpose
    This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.
    Depositor
    Deborah Leckband
    Publication
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha-catenin FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71709-plasmid-map-sequence-id-142365

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  4. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Publication
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    pBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

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  7. pHBV-Luc

    Plasmid
    #71414
    Purpose
    To see the activity of HBV transcription using Luciferase assay
    Depositor
    Wang-Shick Ryu
    Publication
    Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HBV core promoter, enhancer I, enhancer II and luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    HBV
    Available since
    Dec. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    71414-plasmid-map-sequence-id-132686

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  10. p5E-mfap4

    Plasmid
    #70052
    Purpose
    5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.
    Depositor
    David Tobin
    Publication
    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do
    Insert
    mfap4 promoter
    Use
    Gateway 5' element
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70052-plasmid-map-sequence-id-130081

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  12. Str-KDEL_ManII-SBP-EGFP

    Plasmid
    #65252
    Purpose
    synchronize trafficking of ManII from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptavidin-KDEL and truncated Mannosidase II
    Tags
    EGFP and Streptavidin Binding Protein (SBP)
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    65252-plasmid-map-sequence-id-120582

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  18. TGFB1-bio-His

    Plasmid
    #52185
    Purpose
    Expresses full-length Transforming growth factor beta-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TGFB1 (TGFB1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    52185-plasmid-map-sequence-id-80549

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  19. pBUN421

    Plasmid
    #62204
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    TaU3 and Ubi
    Available since
    Feb. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    62204-plasmid-map-sequence-id-521112

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  20. pSBbi-BP

    Plasmid
    #60512
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and puromycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    60512-plasmid-map-sequence-id-97534

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  25. FRB_SCF2_LD6_CTEV

    Plasmid
    #58879
    Purpose
    MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease
    Depositor
    Joshua Leonard
    Publication
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58879-plasmid-map-sequence-id-94240

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  27. mouse deltaPE Oct4-GFP transgenic reporter

    Plasmid
    #52382
    Purpose
    BAC recombineering generated construct, used as a reporter for mouse Oct4 expression
    Depositor
    Jacob Hanna
    Publication
    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GFP pA
    Oct4 (Pou5f1 Mouse)
    PGK-gb2-Neo
    Expression
    Mammalian
    Mutation
    deleted -300 - -1300bp fragment upstream from Oct…
    Promoter
    Oct4 and PGK+gb2
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    52382-plasmid-map-sequence-id-82025

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  32. mEmerald-Lifeact-7

    Plasmid
    #54148
    Purpose
    Localization: Actin, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Expression
    Mammalian
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    19 Articles

  35. pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid
    #50472
    Purpose
    Gs-coupled rM3D-IRES-mCitrine under the control of GFAP promoter
    Depositor
    Bryan Roth
    Has service
    AAV5
    Insert
    rM3D-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    50472-plasmid-map-sequence-id-75495

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  37. pGFP FAK Y397F

    Plasmid
    #50516
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    protein tyrosine kinase, mutated
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Y397F
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles

  39. pUltra-Smurf

    Plasmid
    #48974
    Purpose
    3rd generation Lentiviral vector with bi-cistronic expression of the blue fluorescent protein AmCyan and the gene of interest.
    Depositor
    Malcolm Moore
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    AmCyan
    Expression
    Mammalian
    Available since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    48974-plasmid-map-sequence-id-255027

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Showing: 9351 - 9400 of 177170 results