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162,160 results

Showing: 9401 - 9450 of 162160 results

  6. pDS-Red-N1 GABAA beta3

    Plasmid
    #109143
    Purpose
    The expression vector carries the red fluorescent protein tagged at the C-terminus of beta3 subunit of the GABAa receptor.
    Depositor
    Stefano Vicini
    Insert
    GABAa receptor
    Use
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    beta3 subunit of the GABAa receptor
    Promoter
    CMV
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    109143-plasmid-map-sequence-id-210126

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  12. pBABEpuro-CRAF

    Plasmid
    #51124
    Purpose
    mammalian expression of wt CRAF
    Depositor
    Matthew Meyerson
    Insert
    CRAF (RAF1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51124-plasmid-map-sequence-id-261155

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  15. ADORA2B-DuET

    Plasmid
    #213175
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADORA2B (ADORA2B Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213175-plasmid-map-sequence-id-430743

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  16. AAVS1 CAG rtTA3 TauWT 2N4R-EGFP

    Plasmid
    #132389
    Purpose
    Exchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Article
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauWT 2N4R-EGFP (MAPT Human, Synthetic)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    TREtight
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132389-plasmid-map-sequence-id-270333

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  18. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

    Plasmid
    #218508
    Purpose
    integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mNeongreen
    Use
    Tags
    modified ssrA degradation tag
    Expression
    Bacterial
    Mutation
    deletion of MCS containing Plac_lacZa, introducti…
    Promoter
    P23
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218508-plasmid-map-sequence-id-438746

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  22. TFORF1868

    Plasmid
    #141878
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MMP3 (MMP3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141878-plasmid-map-sequence-id-397231

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  26. pBJ-mPar3

    Plasmid
    #53230
    Purpose
    mammalian expression of mouse Par3
    Depositor
    Shaun Coughlin
    Insert
    Par3 (F2rl2 Mouse)
    Use
    Tags
    FLAG and bovine prolactin signal sequence (MDSKGS…
    Expression
    Mammalian
    Mutation
    Deletion of aa1-15 (native signal peptide)
    Promoter
    Available since
    Aug. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. pANAP

    Plasmid
    #48696
    Purpose
    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS
    Depositor
    Peter Schultz
    Article
    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540
    Inserts
    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutant E.coli leucyl synthetase specific for Anap…
    Promoter
    CMV and human H1 promoter
    Available since
    Nov. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    48696-plasmid-map-sequence-id-197837

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  30. pDule-pCNF

    Plasmid
    #85494
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85494-plasmid-map-sequence-id-169861

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  31. OptoEphB2-mCherry

    Plasmid
    #92042
    Purpose
    Expresses OptoEphB2 labeled with mCherry
    Depositor
    Ji Yu
    Insert
    EPHB2 (EPHB2, CRY2 Human, Mustard Weed)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    92042-plasmid-map-sequence-id-182371

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  33. p416-GPD-DDP1

    Plasmid
    #183950
    Purpose
    Expresses yeast DDP1 from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    DDP1 (DDP1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183950-plasmid-map-sequence-id-363371

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  35. mNeonGreen-Giantin

    Plasmid
    #98880
    Purpose
    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Giantin
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    98880-plasmid-map-sequence-id-191166

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  36. pOpen-SP6rpol

    Plasmid
    #165516
    Purpose
    DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    SP6 RNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165516-plasmid-map-sequence-id-338647

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  48. EF1a-mito-dsRED2

    Plasmid
    #174541
    Purpose
    mitochondria targeted dsRED expressed off of EF1a promoter
    Depositor
    Thomas Schwarz
    Insert
    Mito
    Use
    Lentiviral
    Tags
    dsRED
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Oct. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174541-plasmid-map-sequence-id-345057

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  50. AIMTOR A757

    Plasmid
    #140829
    Purpose
    AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757
    Depositor
    Anne Bonnieu
    Article
    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1
    Insert
    AIMTOR A757 (ULK1 Human)
    Use
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Promoter
    Available since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140829-plasmid-map-sequence-id-305143

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Showing: 9401 - 9450 of 162160 results