173,051 results
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PCMV-intron myc Rab6WT
Plasmid#46781Purposeexpression of WT Rab6a in mammalian cellsDepositorAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Neo-CMV-MCL1
Plasmid#202668PurposeMCL1 overexpression vector with G418 resistanceDepositorInsertMCL1 apoptosis regulator, BCL2 family member (MCL1 Human)
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-PKCb1-mEGFP-N1
Plasmid#112265PurposeITRACKbeta/IDOCKSbeta Donor constructDepositorAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
GeNL(Ca2+)_480/pcDNA3
Plasmid#85205Purposeluminescent reporter for calcium signalingDepositorInsertGeNL(Ca2+)_480
ExpressionMammalianPromoterCMVAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag YAP S381A
Plasmid#27377DepositorAvailable SinceMarch 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
PCMV-intron myc Rab6 T27N
Plasmid#46782Purposeexpression of T27N Rab6a in mammalian cellsDepositorInsertRab6 T27N (RAB6A Human)
TagsmycExpressionMammalianMutationT27N dominant negativePromoterCMVAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEJS2697-CMV-Nme2SmuCas9-E932R
Plasmid#228718PurposeExpresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cellsDepositorInsertNme2SmuCas9-E932R nuclease
UseCRISPRExpressionMammalianMutationNme2-Cas9, Smu PID, E932RPromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB
Plasmid#216233PurposeS. aureus USA300 GyrB Codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialPromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
vDip-C: pAAV-Pcp2-mGreenLantern-KASH
Plasmid#207613PurposevDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cellsDepositorInsertmGreenLantern
UseAAVTagsKASHPromotermouse Pcp2 promoter (L7-6)Available SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_UCP2
Plasmid#132286PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertUCP2 (UCP2 Human)
ExpressionMammalianAvailable SinceNov. 13, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX3X-RanBP2dFG
Plasmid#53120PurposeE.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activityDepositorInsertRanBP2 fragment (RANBP2 Human)
TagsGSTExpressionBacterialMutationfragment (amino acids 2553-2838)PromotertacAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKLBac-Bat-FluA-trimer
Plasmid#229858PurposeInfluenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)DepositorInsertsPA_Bat-FluA_AFC35437.1
PB1_Bat-FluA_AFC35436
PB2_Bat-FluA_AFC35435.1
ExpressionInsectAvailable SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:icre-p2a-tomato
Plasmid#135217PurposeCre coexpressed with tdTomato in macrophagesDepositorInsertmfap4 promoter with iCre and Tomato
UseCre/Lox; Zebrafish expressionAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DYRK3-2A-GFP
Plasmid#118286PurposeExpresses mouse DYRK3 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.DepositorInsertdual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (Dyrk3 Mouse)
UseAAVTags2A peptide and GFPExpressionMammalianPromoterCMV with beta global intronAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK33-GW-TurboID-V5
Plasmid#196281PurposeGateway destination vector to express C-terminal TurboID fusion under metallothionein promoter, for making stable cell linesDepositorTypeEmpty backboneUseGateway CloningAvailable SinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE
Plasmid#139086PurposeExpresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.DepositorInsertSpCas9
UseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato
Plasmid#180152PurposetdTomato cre reporter with Rosa26 recombineering homology armsDepositorInserttdTomato
UseCre/Lox and Mouse TargetingExpressionBacterial and MammalianAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-LGALS1
Plasmid#175755PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS1DepositorInsertLGALS1 (LGALS1 Human)
UseLentiviralAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-285
Plasmid#31060DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseExpressionMammalianMutation-285 to -1 of P2 HNF4A promoterAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMV-BC0547-luc2
Plasmid#214665Purposebarcoded assay, Ca2+ sensorDepositorInsert6x clustered NFAT element with barcode 0547
UseLuciferaseExpressionMammalianAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
NMUR1-Tango
Plasmid#66446PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
MLV Gag-CFP
Plasmid#1812DepositorAvailable SinceMay 19, 2005AvailabilityAcademic Institutions and Nonprofits only -
mCherry-7aa-ActA-pEGFP-C1
Plasmid#177406PurposeTo express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PSMD10
Plasmid#31332DepositorInsertPSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)
TagsHis and TEVExpressionBacterialAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
mGLDC
Plasmid#72881PurposeoverexpressionDepositorAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD
Plasmid#196086PurposeInducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.DepositorInsertgRNA targeting lncRNA NORAD
UseCRISPRExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNC Wnt-1HA
Plasmid#18027DepositorAvailable SinceJuly 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CABC1
Plasmid#23609DepositorInsertCABC1 (COQ8A Human)
UseGateway CloningAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-PCNA-19-SV40NLS-4
Plasmid#55386PurposeLocalization: Nucleus, Excitation: 433, Emission: 475DepositorAvailable SinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
psgRNA2.02-MS2-ccdB
Plasmid#99911PurposeBasic cloning vector for the expression of MS2 inserted sgRNADepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
XE49 pt Beta-catenin-myc
Plasmid#16840DepositorInsertpt beta-catenin-myc (ctnnb1.L Frog)
UseXenopus expressionTags6x mycMutationfour point mutations introduced into the GSK-3 ph…Available SinceJune 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMK33-V5-TurboID-GW
Plasmid#196282PurposeGateway destination vector to express N-terminal TurboID fusion under metallothionein promoter, for making stable cell linesDepositorTypeEmpty backboneUseGateway CloningAvailable SinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES_iSeroSnFR_cytosolic
Plasmid#214132PurposeFluorescent biosensor for intracellular serotonin (5-HT)DepositorInsertiSeroSnFR_cytosolic
TagsMycExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-zeoCV
Plasmid#73890Purposefission yeast drug selection markerDepositorInsertzeoCV
ExpressionBacterial, Mammalian, and Y…PromoterCMVAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
PA-TagRFP-Alpha-5-Integrin-12
Plasmid#62791PurposeLocalization: Focal Adhesions, Excitation:N/A, Emission:N/ADepositorAvailable SinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
N-TAP A2A-YFP pcDNA3
Plasmid#67852Purposemammalian expression of A2A receptor with N terminal TAP tag and C terminal YFP fusionDepositorAvailable SinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK CXCR4 1P
Plasmid#11309DepositorInsert1 perfect bind site for CXCR4 siRNA antisense
UseLuciferaseTagsRr-lucAvailable SinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-Sestrin2-S190W
Plasmid#73670PurposeoverexpressionDepositorInsertSesn2 (SESN2 Human)
TagsFLAGExpressionMammalianMutationS190W changed serine 190 to tryptophanPromoterCMVAvailable SinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-35S_Long-6
Plasmid#197698PurposePhytobrick (MoClo) Level 0 PartDepositorInsert35S promoter (1.3kb)
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only
