165,343 results

1. plentiCMV-sp-HPV-TM(ICAM)

   Plasmid

   #196510

   Purpose

   Express HPV antigen epitope with ICAM transmembrane domain

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   a HPV epitope with CD8a stalk linker and ICAM TM domain
   

   Use

   Lentiviral

   Tags

   3xflag tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-Luciferase-IRES-NrasG12D

   Plasmid

   #60834

   Purpose

   Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

   Depositor

   Scott Lowe

   Article

   Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

   Insert

   NrasG12D (Nras Mouse)
   

   Use

   Retroviral

   Available Since

   Nov. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pP2A-mCherry-N1

   Plasmid

   #84329

   Purpose

   Encodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

   Insert

   P2A-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMG36E

   Plasmid

   #133853

   Purpose

   Expression vector

   Depositor

   Jan Kok, Gerard Venema

   Article

   van de Guchte et al Appl Environ Microbiol. 1989 Jan;55(1):2

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   P32

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 763 pME mApple

   Plasmid

   #195960

   Purpose

   Gateway pME fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mApple
   

   Use

   Other

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcGP67A-mMD2-proA

   Plasmid

   #187878

   Purpose

   baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

   Depositor

   Bruce Beutler

   Article

   Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

   Insert

   MD2 (Ly96 Mouse)
   

   Expression

   Insect

   Mutation

   includes residues 19–160 of mouse MD-2

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-TREtight-mTagBFP2-B19G

   Plasmid

   #100799

   Purpose

   helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

   Depositor

   Ian Wickersham

   Article

   Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

   Has Service

   AAV1

   Insert

   mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TREtight

   Available Since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. RMCE K>R DNA-PKcs

   Plasmid

   #233917

   Purpose

   Low copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.

   Depositor

   Katheryn Meek

   Article

   Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

   Insert

   human DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)
   

   Expression

   Mammalian

   Mutation

   K3753R

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-ERBB2

   Plasmid

   #23888

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pLV[Exp]-EF1A>Tet3G:IRES:Neo

    Plasmid

    #198756

    Purpose

    Vector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418

    Depositor

    Nael Nadif Kasri

    Article

    Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

    Insert

    Tet3G, IRES, Neo
    

    Use

    Lentiviral

    Promoter

    EF1A

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-Camk2-NES-Twitch-NR

    Plasmid

    #173043

    Purpose

    TnC-based calcium sensor with green-red FRET pair ratiometric readout

    Depositor

    Yiyang Gong

    Article

    Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

    Insert

    NES-Twitch-NR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CamK2

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. HR180-LGR5-GFP

    Plasmid

    #129092

    Purpose

    KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

    Depositor

    Toshiro Sato

    Article

    Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

    Insert

    EGFP and LGR5 homology arms (LGR5 Human)
    

    Use

    Homologous recombination donor plasmid

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-mScarlet-I

    Plasmid

    #159172

    Purpose

    To express a bright monomeric red FP for labelling and detection of mammalian cells

    Depositor

    Pantelis Tsoulfas

    Insert

    mScarlet-I
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Mutation

    Codons were optimized to Human codon usage

    Promoter

    CMV

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICSL60004

    Plasmid

    #117519

    Purpose

    rbcS-E9 terminator Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GCTT:E9t:CGCT:BsaI
    

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. DNA-PKcs PQR>ala

    Plasmid

    #83319

    Purpose

    human DNA-PKcs PQR>ala

    Depositor

    Katheryn Meek

    Article

    Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Expression

    Mammalian

    Mutation

    5 phosphorylation sites in the PQR cluster substi…

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

    Plasmid

    #168290

    Purpose

    CRISPR-activator Suntag-P65-HSF1 (SPH)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    dCas9, Suntag-P65-HSF1
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-puro Ras V12

    Plasmid

    #1768

    Depositor

    Bob Weinberg

    Insert

    ras V12 (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G12V, constitutively active

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMC-9-PinII-11

    Plasmid

    #197769

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    PinII terminator
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQE80l ACA

    Plasmid

    #196471

    Purpose

    Protein expression of SpyTag-ELP-CarHC-ELP-SpyTag

    Depositor

    Fei Sun

    Article

    Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

    Insert

    ACA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVUHshp53

    Plasmid

    #11653

    Purpose

    3rd generation lentiviral transfer vector expressing shp53 under an hUbiquitin promoter.

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    GFP, shRNA against TP53 (TP53 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLenti-hSyn1-mScarlet/CAAX

    Plasmid

    #197592

    Purpose

    Lentiviral transfer vector for the neuronal expression of membrane-bound mScarlet (good visualization of neuronal processes)

    Depositor

    Patrik Verstreken

    Insert

    mScarlet-CAAX
    

    Use

    Lentiviral

    Tags

    CAAX

    Promoter

    hSynI

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. DNA-PKcs ABCDE>A

    Plasmid

    #83318

    Purpose

    human DNA-PKcs/ABCDE>ala

    Depositor

    Katheryn Meek

    Article

    Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Expression

    Mammalian

    Mutation

    6 phosphorylation sites in the ABCDE cluster subs…

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TFORF2660

    Plasmid

    #142350

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBECKP-km

    Plasmid

    #117235

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid

    #220612

    Purpose

    Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-KORD-P2A-ArgiNLS-AausFP1
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTRIP-SFFV

    Plasmid

    #235547

    Purpose

    To express GFP

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEP PolI

    Plasmid

    #11722

    Depositor

    Lawrence Loeb

    Article

    Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

    Insert

    DNA polymerase I EP (polA E. coli)
    

    Expression

    Bacterial

    Mutation

    D424A, I709N, A759R. Error-prone version of PolI.

    Available Since

    Aug. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pENTR221-JUN

    Plasmid

    #79512

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human JUN CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    JUN (JUN Human)
    

    Use

    Multisite gateway entry clone

    Tags

    HA Tag

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Orai1-GCaMP6f

    Plasmid

    #73564

    Purpose

    Green fluorescent reporter of Orai1-associated calcium influx

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

    Insert

    Orai1-GCaMP6f (ORAI1 Human, Synthetic)
    

    Tags

    GCaMP6f

    Expression

    Mammalian

    Promoter

    CMV Immediate Early

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. RabV CVS-N2c(deltaG)-tdTomato

    Plasmid

    #73462

    Purpose

    Expresses tdTomato for monosynaptic circuit mapping

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    tdTomato
    

    Use

    Neurotropic virus

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA Set9 wt

    Plasmid

    #24084

    Depositor

    Danny Reinberg

    Article

    Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

    Insert

    Set9 (SETD7 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDNA3-hTLR4-VenusFlag

    Plasmid

    #197640

    Purpose

    Human TOLL protein expression in mammalian cells

    Depositor

    Haian Fu

    Insert

    TLR4 (TLR4 Human)
    

    Tags

    Venus-Flag

    Expression

    Mammalian

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Twitch-2B pcDNA3

    Plasmid

    #49531

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-2B
    

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pXPR_118

    Plasmid

    #113667

    Purpose

    Lentiviral vector expressing dCas9

    Depositor

    John Doench

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Mutation

    catalytically inactive

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CL20_mEGFP_NUP98_HOXD13

    Plasmid

    #205871

    Purpose

    Express mEGFP-tagged fusion protein, NUP98_HOXD13 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    NUP98_HOXD13 (NUP98, HOXD13 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-EF1a-mCherry-P2A-NLSFlpN-nMagHigh

    Plasmid

    #180585

    Purpose

    PA-Flp

    Depositor

    Won Do Heo

    Article

    Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

    Insert

    Split FlpO
    

    Use

    AAV

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. R57C10-Flp2

    Plasmid

    #63172

    Purpose

    R57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusion

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    yeast wild type containing a Glycine at amino acid 5 of the protein sequence
    

    Expression

    Insect

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pAAV-EF1a-pMagHigh-FlpC

    Plasmid

    #180586

    Purpose

    PA-Flp

    Depositor

    Won Do Heo

    Article

    Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

    Insert

    Split FlpO
    

    Use

    AAV

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUltra-5HTP

    Plasmid

    #178042

    Purpose

    Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon

    Depositor

    Han Xiao

    Article

    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

    Inserts

    Modified ScTrpRS for 5HTP incoporation
    suppressor tRNA for 5HTP incorporation
    

    Expression

    Bacterial

    Mutation

    T107C, P254T, C255A

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDZ2FE_Champ1_1-812

    Plasmid

    #105675

    Purpose

    Expresses Flag-tagged full-length human Champ1 protein

    Depositor

    Thanos Halazonetis

    Insert

    Champ1 (CHAMP1 Human)
    

    Tags

    2X Flag tag

    Expression

    Mammalian

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge