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161,202 results

Showing: 721 - 760 of 161202 results

  7. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215010
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215010-plasmid-map-sequence-id-429275

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  8. pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

    Plasmid
    #224497
    Purpose
    Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    224497-plasmid-map-sequence-id-447355

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  9. pIVTR(T7G)-NS1-TX91

    Plasmid
    #172289
    Purpose
    In vitro transcription of NS1 Influenza A virus protein (strain Texas91) for enhanced in vitro transcribed mRNA translation
    Depositor
    Patrik Verstreken
    Insert
    NS1-TX91
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter G-initiating
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172289-plasmid-map-sequence-id-340308

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  10. MSP1673

    Plasmid
    #65769
    Purpose
    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    Use
    CRISPR
    Tags
    NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65769-plasmid-map-sequence-id-112659

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  11. TFORF3148

    Plasmid
    #144624
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFE2 (NFE2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144624-plasmid-map-sequence-id-347054

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  12. pD4sE_SC12

    Plasmid
    #219721
    Purpose
    DV4 stable E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV4 sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A259W, S262R, F279W, A280P, G29K, T33V, A2…
    Promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219721-plasmid-map-sequence-id-433923

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  13. pD1sE_SC12

    Plasmid
    #219718
    Purpose
    DV1 E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV1 sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A259W, T262R, F279W, A280P, S29K, T33V, A2…
    Promoter
    Available since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    219718-plasmid-map-sequence-id-433425

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  14. pZVsE_SC12

    Plasmid
    #219722
    Purpose
    Zika virus stable E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    ZKV sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A264W, T267R, S286W, S287P, G29K, A25M
    Promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219722-plasmid-map-sequence-id-433912

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  15. pD2sE_SC12

    Plasmid
    #219719
    Purpose
    DV2 stable E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV2 sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A259W, T262R, F279W, T280P, S29K, T33V, A2…
    Promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219719-plasmid-map-sequence-id-433926

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  21. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

    Plasmid
    #215014
    Purpose
    Express an autophagy (LC3) reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    MAP1LC3B (Map1lc3b Rat)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215014-plasmid-map-sequence-id-429267

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  22. TFORF2049

    Plasmid
    #143866
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PURA (PURA Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    143866-plasmid-map-sequence-id-392320

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  23. NUDT3A

    Plasmid
    #42348
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT3A (NUDT3 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. SPDK3860

    Plasmid
    #199245
    Purpose
    Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene
    Depositor
    Savithramma Dinesh-Kumar
    Article
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
    Use
    T-dna vector
    Tags
    Expression
    Mutation
    Promoter
    pPEBV
    Available since
    June 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    199245-plasmid-map-sequence-id-396551

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  31. pKT2-ACVR1-G328V-IRES-Katushka

    Plasmid
    #135002
    Purpose
    Expresses ACVR1 with a G328V mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Article
    Mendez et al Clin Cancer Res. 2020 Aug 1;26(15):4080-
    Insert
    Activin A receptor, type I (ACVR1) G328V (ACVR1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    c.983G>T, p.G328V
    Promoter
    Available since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    135002-plasmid-map-sequence-id-271117

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  35. pKS1576

    Plasmid
    #239524
    Purpose
    Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    ScVba2p-mTurquoise2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239524-plasmid-map-sequence-id-475895

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  40. pHR_SFFV_H2B_miRFP703

    Plasmid
    #231951
    Purpose
    Lentiviral vector for constitutive expression of the far-red fluorescent protein miRFP703 fused with the H2B nuclear localization tag (SFFV promoter)
    Depositor
    Leonardo Morsut
    Insert
    H2B_miRFP703
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    231951-plasmid-map-sequence-id-470260

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Showing: 721 - 760 of 161202 results