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167,150 results

Showing: 9851 - 9900 of 167150 results

  4. pUBC-SV40NLS-Pfv-Sapphire

    Plasmid
    #203653
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203653-plasmid-map-sequence-id-402484

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  6. AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR

    Plasmid
    #191795
    Purpose
    vector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13d
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    A134V, A140V, A141V, A143V
    Promoter
    EFS, hU6
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191795-plasmid-map-sequence-id-375580

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  16. GRM5-DuET

    Plasmid
    #213303
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM5 (GRM5 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213303-plasmid-map-sequence-id-431813

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  17. TFORF1216

    Plasmid
    #144090
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NHLH1 (NHLH1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144090-plasmid-map-sequence-id-391502

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  18. pFastBac1-His10FLAG

    Plasmid
    #157736
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Tags
    His10 + 3xFLAG, enterokinase-cleavable
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    157736-plasmid-map-sequence-id-333926

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  22. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100087-plasmid-map-sequence-id-197556

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  25. pHIV-dTomato

    Plasmid
    #21374
    Purpose
    self-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomato
    Depositor
    Bryan Welm
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 19, 2009
    Availability
    Academic Institutions and Nonprofits only
    21374-plasmid-map-sequence-id-292244

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  27. TFORF2546

    Plasmid
    #144209
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CEBPA (CEBPA Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144209-plasmid-map-sequence-id-404084

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  31. PB CAG-mTagBFP2-T2A-iCreERT2

    Plasmid
    #177308
    Purpose
    CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox; Piggybac
    Expression
    Mammalian
    Available Since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177308-plasmid-map-sequence-id-348855

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  33. pSR47S Cm

    Plasmid
    #41892
    Depositor
    Howard Steinman
    Type
    Empty backbone
    Use
    Bacterial allelic exchange vector with sacb
    Expression
    Bacterial
    Available Since
    May 1, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. pLB

    Plasmid
    #11619
    Purpose
    pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.
    Depositor
    Stephan Kissler
    Article
    Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    mouse U6
    Available Since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11619-plasmid-map-sequence-id-274493

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  42. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

    Plasmid
    #212963
    Purpose
    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_GFP targeting spacer
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212963-plasmid-map-sequence-id-422499

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  48. p_m16S

    Plasmid
    #160549
    Purpose
    Used for mycoplasma detection by qPCR. 16S insert truncated internally from the strain Mycoplasma capricolum subsp. capricolum strain California Kid. ATB: Carbenicilline
    Depositor
    Denis Gerlier
    Article
    Jean et al PLoS One. 2017 Feb 22;12(2):e0172358. do
    Insert
    16S rDNA fragment from M. capricolum subsp. capricolum strain California Kid (gi_83283139)
    Use
    Unspecified
    Available Since
    Dec. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    160549-plasmid-map-sequence-id-319414

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Showing: 9851 - 9900 of 167150 results