We narrowed to 10,445 results for: T7
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Plasmid#83918Purposehuman Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag IIDepositorInsertDoublecortin isoform b (DCX NM_178152.2, Human)
UseTags6-His and EGFP+Strep-tag IIExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBK.013
Plasmid#187213PurposeExpresses retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT]), and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT])
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FAM86A
Plasmid#85117Purposebacterial expression of FAM86ADepositorInsertEEF2KMT (FAM86A Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
VGFR1_HUMAN_D0
Plasmid#79743PurposeThis plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVGFR1
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-6
Plasmid#172771PurposeBacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-6
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
p53-(1-393)-R175H-N268D
Plasmid#35086DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains aa 1-393 - R175H-N268DPromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hHBEGF
Plasmid#199234PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProheparin-binding EGF-like growth factor (HBEGF Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
UBQLN2_450-624W
Plasmid#176852PurposeExpresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cellsDepositorInsertUBQLN2_450C_W (UBQLN2 Human)
UseTagsExpressionBacterialMutationdeletion of residues 1-449 and addition of C-term…PromoterT7Available sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-TNKS-2
Plasmid#154149PurposeFor making TNKS-2 RNADepositorInsertTankyrase 2 (TNKS2 Human)
UseIn vitro transcriptionTagsFLAGExpressionMutationPromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-StefinA
Plasmid#182403PurposeBacterial expression of Vesicle Nucleating peptide-stefinA fusionDepositorInsertVNp-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-mCherry-FLARE-Cameleon
Plasmid#123352PurposeRed-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.DepositorInsertmCherry-mCherry-FLARE-Cameleon
UseTags6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET23A-Fucosidase-E1_10125-Ruminococcus_gnavus-His
Plasmid#207665PurposeExpresses fucosidase E1_10125 from Ruminococcus gnavus in BL21 cells, purified using His-tagDepositorInsertFucosidase E1_10125
UseTagsHis-tagExpressionBacterialMutationamino acids 34-584 were usedPromoterT7 promoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBK.014
Plasmid#187215PurposeExpresses retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG]), and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG])
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
HOXB2 (human) HIS-tag pET
Plasmid#8522DepositorInsertHOXB2 (HOXB2 Human)
UseTagsHis and T7ExpressionBacterialMutationStarts at AA #2; makes protein by WesternPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)
Plasmid#153442PurposeE. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.DepositorInsertRc.XDocB
UseTags3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-dSpCas9-HF1-VP64-6xHis
Plasmid#92118PurposeExpression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, Q926APromoterT7Available sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG8Ha-SERBP1(149-400)
Plasmid#176516PurposeExpresses the human C-terminal SERBP1 149-400 construct.DepositorInsertSERBP1 149-400 (SERBP1 Human)
UseTagsHis8 tag, linker, TEV siteExpressionBacterialMutationdeleted amino acids 1-148 and 401-408PromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains1
Plasmid#78461PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2149-2170; Q2513R (please see…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAK7_HUMAN_D0
Plasmid#79706PurposeThis plasmid encodes the kinase domain of PAK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertPAK7 (PAK5 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CEP55_EABR
Plasmid#178469PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCEP55_EABR (CEP55 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI2(8-193)
Plasmid#60356PurposeExpress the RBD of human MSI2 in bacterial cells for protein purificationDepositorInserthuman MSI2 (8-193) (MSI2 Human)
UseTagsHis and TEV protease siteExpressionBacterialMutationcontains amino acids 8-193 of MSI2PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-InPAkt
Plasmid#181915PurposeIndicator of Phosphoinositides using Akt; targeted to the plasma membrane.DepositorInsertpm-InPAkt
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationContains the following mutations with respect to …PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO BROX 1-411;
Plasmid#89602PurposeExpresses full length human Brox in bacteria; internal ID WISP11-297DepositorInsertBROX (BROX Human)
UseTagsHis Tag, V5 tagExpressionBacterialMutationPromoterT7Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s-T78H
Plasmid#100021PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78HDepositorInsertsfMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG5-Myr-FLAG-p110α-DM
Plasmid#55713Purposeeukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227ADepositorInsertp110 alpha (Pik3ca Mouse)
UseTagsmyristoylation tag (Myr-FLAG)ExpressionMammalianMutationT207D, K227APromoterT7Available sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Trx-PP-hNF2
Plasmid#107148Purposebacterial expressionDepositorInsertneurofibromin 2 (NF2 Human)
UseTags(His)6-ThioredoxinExpressionBacterialMutationPromoterT7Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-27
Plasmid#172752PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-27
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132)
Plasmid#73742PurposeExpression of human RPN13 PRU domain in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationPRU domain only (residues 19-132)PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_NoMTS_L6_pET28a+
Plasmid#60012PurposeExpresses TFAM No MTS L6 mutant in bacterial cellsDepositorInsertHuman TFAM No MTS (TFAM Human)
UseTags6xHisExpressionBacterialMutationMissing 1st 42 aas (Mitochondrial targeting seque…PromoterT7Available sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
MK13_HUMAN_D0
Plasmid#79693PurposeThis plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK13
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB527
Plasmid#74869PurposeGateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (72P)
Plasmid#62083PurposeExpression of human p53 (1-73) (72P) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHIsExpressionBacterialMutationcontains TAD residues 1-73; 72PPromoterT7Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)-SPN+IFS
Plasmid#83372PurposeExpresses SPN and IFS complex in E. coli cellDepositorInsertsSPN
IFS
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB525
Plasmid#74867PurposeGateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST15.1-hRPN13 (285-407)
Plasmid#61934PurposeExpression of hRPN13 (285-407) in E.coliDepositorInserthRPN13 (285-407) (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationConstruct starts at residue 285PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM41_KEAP1_KELCH
Plasmid#178476PurposeBacterial expression of human domain with His-tag and MBP tagDepositorInsertKEAP1_KELCH (KEAP1 Synthetic, Human)
UseTagsHis-MBPExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_EPS15_EH
Plasmid#178475PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertEPS15_EH (EPS15 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR4-Dact1(1250-1601)
Plasmid#52046PurposeTo generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.DepositorInsertDact1(1250-1601) (Dact1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEM-PH-citrine
Plasmid#131406PurposePurpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrineDepositorInsertPH
UseIn vitro transcriptionTagscitrine yellow fluorescent proteinExpressionMutationPromoterT7Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
VRK3_HUMAN_D0
Plasmid#79681PurposeThis plasmid encodes the kinase domain of VRK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVRK3 (VRK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only