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171,998 results

Showing: 10451 - 10500 of 171998 results

  3. OmpA3-PNGaseF-TEV-His6

    Plasmid
    #114274
    Purpose
    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.
    Depositor
    Martin Caffrey
    Insert
    Peptide: N-glycosidase F
    Tags
    OmpA3 leader sequence (for periplasmic localisati…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    114274-plasmid-map-sequence-id-218322

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  9. BDNF mCherry

    Plasmid
    #245052
    Purpose
    Mammalian secretory granule marker using BDNF mCherry marker
    Depositor
    Thomas Martin
    Insert
    BDNF (BDNF Mouse)
    Tags
    mCherry
    Expression
    Mammalian
    Available Since
    Nov. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    245052-plasmid-map-sequence-id-487700

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  10. HiBiT-TP53(Y220C) Fusion Vector

    Plasmid
    #236914
    Purpose
    Express HiBiT-TP53(Y220C) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    TP53 (TP53 Human)
    Tags
    HiBiT
    Expression
    Mammalian
    Mutation
    Y220C
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236914-plasmid-map-sequence-id-473393

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  11. pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233371
    Purpose
    EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038
    Inserts
    NES-Kinprola_PKA-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Promoter
    EF1α
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233371-plasmid-map-sequence-id-466776

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  15. NanoBRET Assay Vector, HaloTag-KRAS(G12V)

    Plasmid
    #238570
    Purpose
    Express HaloTag-KRAS(G12V) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238570-plasmid-map-sequence-id-485890

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  23. Gi3-CASE

    Plasmid
    #168122
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A114/E115 within GNAI1
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168122-plasmid-map-sequence-id-332862

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  25. pDD162 (Peft-3::Cas9 + Empty sgRNA)

    Plasmid
    #47549
    Purpose
    Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    Empty sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available Since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47549-plasmid-map-sequence-id-228099

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  26. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

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  27. CCR2-DuET

    Plasmid
    #213198
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR2 (CCR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    213198-plasmid-map-sequence-id-431752

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  28. pROF148

    Plasmid
    #155330
    Purpose
    Binary vector for expression of the PULSE system under the control of AtUbi10 promoter. It contains a plant selection cassette. 
    Depositor
    Matias Zurbriggen
    Article
    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi
    Insert
    LB_Tnos-nptII-Pnos_PAtUbi10-SRDX-NLS-EL222-Tnos_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    155330-plasmid-map-sequence-id-311083

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  31. MSCV IRES Luciferase

    Plasmid
    #18760
    Depositor
    Scott Lowe
    Type
    Empty backbone
    Use
    Luciferase and Retroviral
    Expression
    Mammalian
    Available Since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only
    18760-plasmid-map-sequence-id-169127

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  33. CXCR5-DuET

    Plasmid
    #213222
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR5 (CXCR5 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213222-plasmid-map-sequence-id-430784

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  39. pcDNA3-mRuby2

    Plasmid
    #40260
    Purpose
    RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Article
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has Service
    Cloning Grade DNA
    Insert
    mRuby2
    Tags
    His6 and Xpress
    Expression
    Mammalian
    Promoter
    CMV IE
    Available Since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    40260-plasmid-map-sequence-id-243381

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  42. pDM028

    Plasmid
    #216809
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216809-plasmid-map-sequence-id-430135

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  43. 3xFLAG-dCas9/p-bacteria

    Plasmid
    #64325
    Purpose
    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do
    Insert
    3xFLAG-dCas9
    Use
    CRISPR; Tetracycline-inducible
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pLtetO-1
    Available Since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64325-plasmid-map-sequence-id-107969

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Showing: 10451 - 10500 of 171998 results