19,270 results
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pAAV-CWB-yellow pre-eGRASP(p30)
Plasmid#111587PurposeAn AAV vector that expresses yellow pre-eGRASP(p30) under the CaMKIIa promoter.DepositorInsertyellow pre-eGRASP(p30)
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro
Plasmid#18750DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-mRuby2
Plasmid#99126PurposeAn AAV genome that expresses the fluorescent protein mRuby2 from the hSyn1 promoterDepositorInsertmRuby2
UseAAVTagsExpressionMammalianMutationPromoterhSyn1Available sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-1:PXN-EGFP
Plasmid#87420PurposeHomology arms and linker-EGFP sequence for C-terminus tagging of human PXNDepositorInsertPXN Homology Arms with linker-EGFP (PXN Human)
UseCRISPR; Donor templateTagslinker-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA
Plasmid#171380PurposeExpression vector for encoding a human codon-optimized RfxCas13d driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorInserthumanized RfxCas13d
UseTagsHAExpressionMammalianMutationPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pST-K
Plasmid#44560Purposefor ectopic constitutive expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterUV15 promoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/v5-DEST hGlut1
Plasmid#18085DepositorInsertGlut1 (SLC2A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
RANLS
Plasmid#50843PurposeExpresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.DepositorInsertddRFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp3-His
Plasmid#11821DepositorInsertCaspase 3 (CASP3 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b
Plasmid#80612PurposepBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNICKclos1.0
Plasmid#73639PurposeGenome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824DepositorInsertsCas9 nickase
sGRNA to pyrE
UseE.coli-clostridium shuttle vectorTagsExpressionMutationD10APromoterj23119 and ptbAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDSG419
Plasmid#115600Purposesurface-displayed Ag2; medium copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 HSPA8
Plasmid#19514DepositorInsertheat shock 70kDa protein 8 (HSPA8 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pC1-HyPer-2
Plasmid#42211PurposeA genetically encoded sensor for H2O2 with expanded dynamic range.DepositorInsertHYPER 2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-OsU6c
Plasmid#66197Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterOsU6cAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV CMV-TERRA
Plasmid#111375PurposeTERRA expression contains approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequencesDepositorInsertTERRA polyA
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Oct3/4-IRES-Neo
Plasmid#13461DepositorInsertOct3/4 (Pou5f1 Mouse)
UseTagsExpressionMammalianMutationP31SPromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mSncg-EGFP
Plasmid#153163PurposeMouse gamma-synuclein (mSncg) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromotermSncgAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 S
Plasmid#149329PurposeGateway-compatible Entry vector, with insert of the spike (S) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS (S SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223-SRC
Plasmid#23934DepositorInsertSRC (SRC Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
punc503GFP
Plasmid#64021Purpose503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expressionDepositorInsertunc-45b: GFP (unc45b Zebrafish)
UseGatewayTagsExpressionMutationPromoter503-bp promoter of zebrafish unc-45bAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Tubulin-C-18
Plasmid#54292PurposeLocalization: Microtubules, Excitation: 487, Emission: 509DepositorInsertTubulin (TUBA1B Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1_pMagFast2(3x)-iRFP
Plasmid#67297PurposeBlue light dependent photoswitching systemDepositorInsertpMagFast2(3x)-iRFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR iSH-YFP-PIF
Plasmid#50841PurposeOptogenetic PI3K activation system; cytoplasmic componentDepositorInsertsp85 iSH2
PIF6 1-100
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAd-RFP
Plasmid#12520DepositorInsertRFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
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MCP-YFP
Plasmid#101160Purposeto express MCP-YFP fusion proteinDepositorInsertMCP-YFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLH-stsgRNA3.1
Plasmid#64118PurposeVector for expression of St1 sgRNA3.1 in mammalian cellsDepositorInsertSt1 sgRNA3.1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-h_bPAC_cmyc
Plasmid#28134Purposeoptogenetic tool for light modulation of cellular cAMPDepositorInserthumanized photoactivated adenylyl cyclase
UseIn vitro transcriptionTagsMycExpressionMutationPromoterAvailable sinceJune 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TRIM28
Plasmid#45568DepositorInsertTRIM28 (TRIM28 Human)
UseTagsEGFPExpressionMammalianMutationG34S mutation in EGFPPromoterCMVAvailable sinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
LacZ in pLX304
Plasmid#42560DepositorInsertLacZ
UseLentiviralTags2xV5ExpressionMutationPromoterCMVAvailable sinceMarch 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
c-myc promoter (TBE 1/2 - wt)
Plasmid#16595DepositorInsertc-myc promoter (TBE 1/2 wt) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKIKOarsBKm
Plasmid#46766PurposeE.coli genomic integration at arsBDepositorTypeEmpty backboneUseE.coli genomic integrationTagsExpressionMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Flag-H2A
Plasmid#63560PurposeExpression of human H2A tagged with FlagDepositorInsertH2A (H2AC13 Human)
UseTagsFlag (MDYKDDDDKVPRIQ)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCE046-SiT-Cas12a-[Repr]
Plasmid#128132PurposeExpress both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcriptDepositorInsertAsCas12a-[Repr]-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-c-Met
Plasmid#31786DepositorInsertMet (MET Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-shRab27a
Plasmid#120930Purposeconditionally knockdown Rab27a in mouse cell linesDepositorInsertshRab27a (Rab27a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-OR51E1
Plasmid#22323DepositorInsertOR51E1 (OR51E1 Human)
UseTagsRhoExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_PsChR2_EYFP
Plasmid#69057PurposeHigh-efficiency blue-shifted cation channelrhodopsinDepositorInsertPsChR2
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
BPK3079
Plasmid#78741PurposeHuman expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)DepositorInsertAsCpf1 crRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
EP-Pol vector
Plasmid#131686PurposeA lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymeraseDepositorInsertAdenovirus DNA polymerase
UseLentiviralTagsHAExpressionMutationF421Y, D827APromoterAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
VN-alpha Synuclein
Plasmid#89470Purposeexpresses human alpha Synuclein fused to the N-terminal part of VenusDepositorInsertSNCA (SNCA Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag p21 T145A
Plasmid#16241DepositorInsertp21 (CDKN1A Human)
UseTagsFlagExpressionMammalianMutationT145APromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-mt-mKeima-P2A-FRB-Fis1
Plasmid#135295Purposestably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cellsDepositorInsertmt-mKeima-P2A-FRB-Fis1 (FIS1 Human)
UseLentiviralTagsExpressionMammalianMutationmt-mKeima is fused with FRB-Fis1 via P2A seqeuncePromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pNCS-CyOFP
Plasmid#74278PurposeConstitutive expression of CyOFP in bacteriaDepositorInsertCyOFP
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCitrine-C1
Plasmid#54587PurposeLocalization: C1 Cloning Vector, Excitation: 516, Emission: 529DepositorTypeEmpty backboneUseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQP-iRIS
Plasmid#102584PurposeRegulation of protein localization (near-infrared-blue-light-inducible shuttle)DepositorInsertBphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mDyn2 pcDNA3
Plasmid#36264DepositorInsertDynamin 2 (Dnm2 Mouse)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only
