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177,145 results

Showing: 1401 - 1450 of 177145 results

  4. pCasSA

    Plasmid
    #98211
    Purpose
    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9
    Depositor
    Quanjiang Ji
    Publication
    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    98211-plasmid-map-sequence-id-485072

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  6. CMV-LUC2CP/intron/ARE

    Plasmid
    #62858
    Purpose
    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Publication
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    insertion of chimeric intron at nueotide poclsiti…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    62858-plasmid-map-sequence-id-243538

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  8. CMV-R-GECO1.2

    Plasmid
    #45494
    Purpose
    Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2
    Depositor
    Robert Campbell
    Publication
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    R-GECO1.2
    Expression
    Mammalian
    Mutation
    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…
    Promoter
    CMV
    Available since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    25 Articles
    45494-plasmid-map-sequence-id-123029

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  11. pcDNA3-CD14

    Plasmid
    #13645
    Depositor
    Doug Golenbock
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD14 (CD14 Human)
    Expression
    Mammalian
    Available since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    13645-plasmid-map-sequence-id-7723

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  15. CMV-LUC2CP/ARE

    Plasmid
    #62857
    Purpose
    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Publication
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    destabilizing sequences (DS) added to the C termi…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13 Articles
    62857-plasmid-map-sequence-id-212900

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  16. pET-MBP-mSA2

    Plasmid
    #52319
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Publication
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Tags
    FLAG, His, MBP, and TEV
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    52319-plasmid-map-sequence-id-237583

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  17. pANAP

    Plasmid
    #48696
    Purpose
    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS
    Depositor
    Peter Schultz
    Publication
    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540
    Inserts
    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    Expression
    Mammalian
    Mutation
    mutant E.coli leucyl synthetase specific for Anap…
    Promoter
    CMV and human H1 promoter
    Available since
    Nov. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    15 Articles
    48696-plasmid-map-sequence-id-197837

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  18. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid
    #46168
    Purpose
    Codon optimized Cas9_SV40 NLS with intron
    Depositor
    John Calarco
    Publication
    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    codon optimized Cas9_SV40 NLS with intron
    Use
    CRISPR
    Mutation
    intron inserted at position 3113-3163, and SV40 N…
    Promoter
    eef-1A.1 (eft-3)
    Available since
    July 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47 Articles
    46168-plasmid-map-sequence-id-65793

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  27. mCherry-CD9-10

    Plasmid
    #55013
    Purpose
    Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD9 (CD9 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    55013-plasmid-map-sequence-id-234553

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  30. pmAmetrine-DEVD-tdTomato

    Plasmid
    #18879
    Purpose
    Caspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomato
    Depositor
    Robert Campbell
    Publication
    Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mAmetrine-DEVD-tdTomato (CASP3 lab constructed, Human)
    Expression
    Mammalian
    Mutation
    mAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…
    Available since
    Aug. 13, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    18879-plasmid-map-sequence-id-148222

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  32. pcDNA3-TLR3-CFP

    Plasmid
    #13641
    Depositor
    Doug Golenbock
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Toll-Like Receptor 3 (TLR3 Human)
    Tags
    CFP
    Expression
    Mammalian
    Available since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    13641-plasmid-map-sequence-id-512615

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  47. pcDNA3.1-Clover-mRuby2

    Plasmid
    #49089
    Purpose
    Clover-mRuby2 tandem fusion for FRET calibration
    Depositor
    Kurt Beam
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Clover-mRuby2 fusion
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    16 Articles
    49089-plasmid-map-sequence-id-520277

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  49. pdCas9::BFP-humanized

    Plasmid
    #44247
    Purpose
    Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
    Depositor
    Stanley Qi
    Publication
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 fused to BFP
    Use
    CRISPR and Retroviral
    Tags
    2xNLS & tagBFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    MSCV LTR promoter
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    44247-plasmid-map-sequence-id-60248

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Showing: 1401 - 1450 of 177145 results