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165,688 results

Showing: 1401 - 1450 of 165688 results

  1. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

    Plasmid
    #208959
    Purpose
    A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208959-plasmid-map-sequence-id-415785

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  3. TFORF2399

    Plasmid
    #144334
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZFPM1 (ZFPM1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    144334-plasmid-map-sequence-id-427297

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  11. Flag-mFoxJ1

    Plasmid
    #122965
    Purpose
    Expresses Flag-tagged mouse FoxJ1 in mammalian cells
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Insert
    mouse FoxJ1 (Foxj1 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    122965-plasmid-map-sequence-id-238008

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  14. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29 (JO582)

    Plasmid
    #208954
    Purpose
    A variant CE1 construct with Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Phi29(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208954-plasmid-map-sequence-id-415743

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  22. mCherry-WASH1-N-18

    Plasmid
    #55163
    Purpose
    Localization: Cytoskeleton, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    WASH1
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. Ubi:ccdb-eGFP + RUBY

    Plasmid
    #233310
    Purpose
    GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and RUBY marker
    Depositor
    Thomas Baum
    Insert
    Ubi:ccdb-eGFP + RUBY
    Use
    Gateway destination
    Tags
    eGFP
    Available Since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233310-plasmid-map-sequence-id-464384

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  30. Ubi:ccdb + RUBY

    Plasmid
    #233306
    Purpose
    GATEWAY compatible vector with soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and RUBY marker
    Depositor
    Thomas Baum
    Insert
    Ubi:ccdb + RUBY
    Use
    Gateway destination
    Available Since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233306-plasmid-map-sequence-id-464366

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  34. pN1-COX8-mScarletI

    Plasmid
    #129341
    Purpose
    Mitochondria matrix localization
    Depositor
    Yasushi Okada
    Insert
    mScarlet(i) (Addgene 85044)
    Expression
    Mammalian
    Available Since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    129341-plasmid-map-sequence-id-260104

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  38. pCDNA3.1 Bach1 S990A

    Plasmid
    #73643
    Purpose
    Mammalian expression of Bach1 S990A mutant (BRCA binding mutant).
    Depositor
    Sharon Cantor
    Insert
    Bach1 (BACH1 Human)
    Tags
    His and Myc
    Expression
    Mammalian
    Mutation
    S990A (BRCA binding mutant)
    Promoter
    CMV
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only

  41. TFORF0943

    Plasmid
    #143246
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    LMO2 (LMO2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    143246-plasmid-map-sequence-id-385782

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  43. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

    Plasmid
    #208952
    Purpose
    A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208952-plasmid-map-sequence-id-415741

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  44. RXFP2-Tango

    Plasmid
    #66493
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    RXFP2 (RXFP2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66493-plasmid-map-sequence-id-310431

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  48. RXFP4-Tango

    Plasmid
    #66495
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    RXFP4 (RXFP4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1401 - 1450 of 165688 results